Guild

HEAD START

FULL DAY HEAD START PROGRAM

PROGRAMMING SUPPORTS A COMPREHENSIVE NETWORK OF COOPERATIVE STUDY UNITS TO FACILITATE COOPERATIVE RESEARCH, TECHNICAL ASSISTANCE, EDUCATION, AND TRAINING TO INFORM SCIENCE-BASED MANAGEMENT OF THE NATIONAL PARK SYSTEM. THE STUDY UNITS FACILITATE MULTI-DISCIPLINARY SCIENCE AND INTEGRATED INFORMATION PRODUCTS ACROSS MULTIPLE GEOGRAPHIC REGIONS OF THE NATIONAL PARK SYSTEM. AS A RESULT OF THIS INFLATION REDUCTION ACT INVASIVE SPECIES-FUNDED PROJECT, FOREST RESTORATION WILL OCCUR ON UP TO 7,000 ACRES ACROSS UP TO 19 NATIONAL PARKS IN THE NORTHEAST REGION, WITH BENEFITS TO LOCAL ECONOMIES AND WATERSHED HEALTH EXTENDING BEYOND PARK BOUNDARIES. THIS PROJECT WILL HELP RESTORE AND MAINTAIN FORESTED ECOSYSTEMS THAT SUPPORT NATIVE PLANTS AND WILDLIFE, AS WELL AS PROVIDE CONTEXT FOR THE IMPORTANT HISTORICAL AND CULTURAL EVENTS WHICH THE NPS INTERPRETS FOR THE BENEFIT OF THE PUBLIC. OTHER BENEFICIARIES INCLUDE STATE AND LOCAL GOVERNMENTS, PUBLIC PRIVATE NONPROFIT ORGANIZATIONS, NONPROFIT INSTITUTIONS OF HIGHER EDUCATION, AND THE PUBLIC.

AMERICAN RESCUE PLAN ACT FUNDING FOR LOOK-ALIKES

HEAD START

RAPID MULTIPLEX METHOD FOR DIRECT PHENOTYPIC ID/AST OF BACTERIAL PATHOGENS

COMMUNITY PROJECT FUNDING/CONGRESSIONALLY DIRECTED SPENDING - CONSTRUCTION

INCREASE ACCESS OF UNDERSERVED AND SMALL ACREAGE LANDOWNERS TO EMERGING PRIVATE MARKETS FOR CLIMATE MITIGATION OR FOREST RESILIENCE THROUGH TRAINING

CONGRESSIONALLY DIRECTED SPENDING FOR CONSTRUCTION PROJECTS - APPLICANT: COUNTY OF SAN JOAQUIN (SAN JOAQUIN HEALTH CENTERS) ADDRESS: 10100 TRINITY PARKWAY, SUITE 100, STOCKTON, CA 95219 PROJECT DIRECTOR: JHOANNA AMIGABLE CONTACT PHONE NUMBER: (209) 953-3712 EMAIL ADDRESS: JAMIGABLE@SJHEALTH.ORG WEBSITE: WWW.SJHEALTH.ORG GRANT PROGRAM FUNDS REQUESTED: $1,810,929 SAN JOAQUIN HEALTH CENTERS (SJ HEALTH) IS A FQHC LOOK-ALIKE PROVIDING COMPREHENSIVE PRIMARY CARE TO THE RESIDENTS OF CALIFORNIA’S SAN JOAQUIN COUNTY. IT CURRENTLY SERVES APPROXIMATELY 31,000 UNIQUE PATIENTS FROM THROUGHOUT SAN JOAQUIN COUNTY ANNUALLY THROUGH 120,000 VISITS AT SEVEN FIXED SITE HEALTH CENTERS IN THE COUNTY (THREE IN FRENCH CAMP, THREE IN STOCKTON, AND ONE IN MANTECA) AND VIA ONE MOBILE CLINIC TARGETING COMMUNITIES AND POPULATIONS (HOMELESS AND MIGRANT AGRICULTURAL WORKERS) WITH BARRIERS TO ACCESSING HEALTH CARE. SJ HEALTH IS REQUESTING FUNDS TO BUILD OUT AND FULLY EQUIP A HEALTH CENTER IN LODI, CALIFORNIA. LODI IS IN THE NORTHERN END OF SAN JOAQUIN COUNTY. IT IS APPROXIMATELY 15 MILES FROM SJ HEALTH’S STOCKTON SITE, 20 MILES FROM ITS FRENCH CAMP SITE, AND 26 MILES FROM ITS MANTECA SITE. IN SPITE OF THE DISTANCE, APPROXIMATELY 970 UNIQUE PATIENTS FROM LODI AND SURROUNDING AREA ZIP CODES HAVE SOUGHT CARE AT SJ HEALTH HEALTH CENTERS THROUGH APPROXIMATELY 4,400 APPOINTMENTS SINCE JANUARY 1, 2022. THE TWO MEDI-CAL MANAGED CARE PLANS IN SAN JOAQUIN COUNT CURRENTLY HAVE ASSIGNED SJ HEALTH APPROXIMATELY 1,300 PATIENTS FROM THE LODI AREA. IT IS ANTICIPATED THAT A SIGNIFICANT PROPORTION OF SJ HEALTH’S PATIENTS FROM THE LODI AREA WILL CHOOSE TO RECEIVE THEIR CARE AT THE LODI HEALTH CENTER. THE COMMUNITY HEALTH NEEDS ASSESSMENT THAT SJ HEALTH COMMISSIONED AS A COMPONENT OF ITS 2022-2024 STRATEGIC PLANNING IDENTIFIED LODI AS ONE OF THE AREAS OF SAN JOAQUIN COUNTY WITH THE HIGHEST CONCENTRATION OF UNSERVED LOW-INCOME PERSONS. USING HRSA AND OTHER LOCAL COVERAGE DATA SOURCES, IT WAS ESTIMATED THAT THERE ARE AT LEAST 3,500 LOW-INCOME PEOP LE IN LODI WHO COULD REASONABLY BE EXPECTED TO SEEK CARE FROM A NEW HEALTH CENTER. THESE NUMBERS DON’T INCLUDE THOSE ALREADY BEING SEEN BY AND ASSIGNED TO SJ HEALTH. SJ HEALTH WILL LEASE AND CONVERT EXISTING OFFICE OR OTHER COMMERCIAL SPACE OF APPROXIMATELY 6,000 SQUARE FEET IN LODI INTO A COMMUNITY HEALTH CENTER, SPECIFICALLY TARGETING THE RESIDENTS OF LODI AND SURROUNDINGS. THE STILL TO BE IDENTIFIED SPACE WILL BE DESIGNED AND CONFIGURED TO PROVIDE A FULLY ACCESSIBLE COMMUNITY HEALTH CENTER OFFERING PRIMARY CARE SERVICES AS WELL AS OPTOMETRY SERVICES FOR THE FIRST TIME IN SJ HEALTH’S HISTORY. WHEN BUILT OUT, THE FULLY EQUIPPED AND SUPPLIED HEALTH CENTER WILL INCLUDE A WAITING AREA, 14 EXAM ROOMS (TWO FOR OPTOMETRY), ADMINISTRATIVE OFFICES, TWO COUNSELING ROOMS, A MEETING/TRAINING ROOM, A STAFF ROOM, AND RESTROOMS. COMMUNITY PROJECT FUNDING WILL COVER THE COSTS OF CONSTRUCTION/RENOVATION AND PURCHASING AND INSTALLING THE NEEDED MEDICAL/OFFICE EQUIPMENT, FURNISHINGS, IT/TELECOMMUNICATIONS EQUIPMENT, AND OPTOMETRY EQUIPMENT. WITHOUT A SPECIFIC SITE UNDER LEASE YET, SJ HEALTH CURRENTLY ESTIMATES THE LODI HEALTH CENTER PROJECT COST AT $1,999,430.05 BASED ON GUIDANCE FROM ITS ARCHITECT. THIS APPLICATION REQUESTS $1,810,929 IN SUPPORT OF THE TOTAL CONSTRUCTION AND ALLOWABLE MOVEABLE EQUIPMENT COSTS. THE BALANCE OF $188,501.05 WILL BE PAID DIRECTLY BY SJ HEALTH OUT OF FUNDS IN HAND. SJ HEALTH WILL COVER THE OTHER COSTS OF OPENING AND ALL COSTS OF OPERATING THE HEALTH CENTER. WITH TWO FAMILY MEDICINE PHYSICIANS, TWO NURSE PRACTITIONERS, AND AN OPTOMETRIST, THE LODI SITE WILL EVENTUALLY PROVIDE PRIMARY MEDICAL CARE AND OPTOMETRY SERVICES TO APPROXIMATELY 3,200 PATIENTS OF ALL AGES (THROUGH 11,000 VISITS) ANNUALLY. SJ HEALTH PROJECTS THAT THE CLINIC WILL REACH THESE NUMBERS AND BECOME SELF-SUSTAINING BASED ON OPERATING REVENUES WITHIN TWO TO THREE YEARS.

DETECTION OF BIODEFENSE PATHOGENS USING PHAGE DIAGNOSTICS

PURPOSE: ECONOMIC DEVELOPMENT INITIATIVE, COMMUNITY PROJECT FUNDING/CONGRESSIONAL DIRECTED SPENDING AWARDS ARE AUTHORIZED UNDER THE CONSOLIDATED APPROPRIATIONS ACT, 2022 PUBLIC LAW 117-328 AND THE EXPLANATORY STATEMENT FOR DIVISION L OF THAT ACT. PROJECTS SELECTED FOR COMMUNITY PROJECT FUNDING/CONGRESSIONAL DIRECTED SPENDING ARE LISTED IN THE JOINT EXPLANATORY STATEMENT (JES) THAT ACCOMPANIES A SPECIFIC FISCAL YEAR’S APPROPRIATIONS ACT OR CONGRESSIONAL RECORD. THE JES LISTS PROJECT, RECIPIENT, STATE, AMOUNT AND CONGRESSIONAL SPONSOR.; ACTIVITIES TO BE PERFORMED: ECONOMIC DEVELOPMENT INITIATIVE, COMMUNITY PROJECT FUNDING/CONGRESSIONALLY DIRECTED SPENDING AWARD PROJECTS INCLUDE A WIDE VARIETY OF ACTIVITIES THAT RESULT IN ECONOMIC DEVELOPMENT OR COMMUNITY DEVELOPMENT OUTCOMES. HUD WILL NOT KNOW THE FULL SCOPE OF THE PROJECT UNTIL THE RECIPIENT SUBMITS THE REQUIRED PROJECT NARRATIVE AND CONFIRMS ALIGNMENT WITH THE LANGUAGE AS PROVIDED IN THE CONGRESSIONAL RECORD. TO FIND THE DETAILS OF THE GRANT AWARD AS WRITTEN WITHIN THE CONGRESSIONAL RECORD USE THE FOLLOWING LINK AND PATH SELECTIONS TO GET TO THE DESCRIPTION OF THE ECONOMIC DEVELOPMENT INITIATIVE, COMMUNITY PROJECT FUNDING GRANTS HTTPS://WWW.HUD.GOV/PROGRAM_OFFICES/COMM_PLANNING/EDI-GRANTS, SELECT THE FISCAL YEAR OF INTEREST, SCROLL DOWN TO PROGRAM LAWS AND REGULATIONS, UNDER FISCAL YEAR 20XX CONSOLIDATED APPROPRIATIONS ACT, 20XX: CONGRESSIONAL RECORD (JOINT EXPLANATORY STATEMENT).; EXPECTED OUTCOMES: COMPLETION OF THE PROJECT AS DESCRIBED IN THE JOINT EXPLANATORY STATEMENT (JES) PROJECT DESCRIPTION AND SUBSEQUENT APPROVED PROJECT NARRATIVE.; INTENDED BENEFICIARIES: THE PROJECT BENEFICIARIES ARE THE INDIVIDUALS AND/OR ORGANIZATIONS THAT ARE AWARDED GRANT FUNDS OR SERVED BY THE ENTITIES THAT ARE AWARDED GRANT FUNDS AS IDENTIFIED IN THE JES RECIPIENT OR PROJECT DESCRIPTION SECTIONS.; SUBRECIPIENT ACTIVITIES: THE SUBRECIPIENT ACTIVITIES ARE UNKNOWN AT THE TIME OF AWARD.

BIOLUMINESCENT REPORTER PHAGE FOR THE DIAGNOSTIC DETECTION OF SHIGELLOSIS

DEVELOPMENT AND DISSEMINATION GRANT PROGRAM

THE USFWS IS RESPONSIBLE FOR MONITORING AND TRACKING CHANGES IN FOREST VEGETATION HABITAT AND HEALTH ACROSS NATIONAL WILDLIFE REFUGES. REFUGES IN THE NORTHERN FOREST ARE LOOKING TO INSTALL LONGTERM MONITORING PLOTS ACROSS REFUGES BUT LACK THE CAPACITY TO PERFORM THIS CRITICAL STEP. ADDITIONALLY NORTHERN FOREST REFUGES NEED HELP WITH PLANNING ASSESSING PREPARING AND ADMINISTERING MANAGEMENT ACTIONS THAT WORK TOWARDS FOREST HABITAT HEALTH AND RESILIENCY GOALS. THE GUILD IS WELL SUITED TO MEET THE USFWS MONITORING AND FOREST MANAGEMENT NEEDS FOR THREE REASONS. FIRST THE GUILD HAS A BROAD NETWORK OF FOREST PRACTITIONERS FOCUSED ON THE TYPES OF MANAGEMENT USFWS REQUIRES. GUILD MEMBERS ARE PARTICULARLY ATTUNED TO THE REQUIREMENTS OF CONVERSATION LANDOWNERS SUCH AS USFWS AND ABLE TO ACCOMMODATE WILDLIFE AND RESILIENCE CONCERNS. SECOND THE GUILD HAS THE ADMINISTRATIVE SYSTEMS IN PLACE TO HANDLE COMPLEX MULTIYEAR FEDERAL CONTRACTS. THE GUILD HAS DECADES OF SUCCESSFUL PARTNERSHIP WITH THE US FOREST SERVICE PROVIDING MONITORING PLANNING AND FOREST MANAGEMENT. IN RECENT YEARS THE GUILD HAS EXPANDED ITS WORK WITH THE NATIONAL PARK SERVICE AND IS WORKING TO REMOVE INVASIVE PLANTS IN CLOSE COOPERATION WITH NPS STAFF. THIRD THE GUILD IS ACTIVELY ADDRESSING FORESTRY WORKFORCE CHALLENGES THROUGH TRAINING AND EARLY CAREER SUPPORT. THIS WORKFORCE DEVELOPMENT IS CRUCIAL BECAUSE IT WILL HELP THE USFWS FILL STEWARDSHIP POSITIONS IN THE LONGTERM.

RURAL HEALTH CARE COORDINATION NETWORK PROGRAM

PROGRAMMING SUPPORTS A COMPREHENSIVE NETWORK OF COOPERATIVE STUDY UNITS TO FACILITATE COOPERATIVE RESEARCH, TECHNICAL ASSISTANCE, EDUCATION, AND TRAINING TO INFORM SCIENCE-BASED MANAGEMENT OF THE NATIONAL PARK SYSTEM. THE STUDY UNITS FACILITATE MULTI-DISCIPLINARY SCIENCE AND INTEGRATED INFORMATION PRODUCTS ACROSS MULTIPLE GEOGRAPHIC REGIONS OF THE NATIONAL PARK SYSTEM. AS A RESULT OF THIS BIPARTISAN INFRASTRUCTURE LAW-ECOSYSTEM RESTORATION PROJECT, FOREST RESTORATION WILL OCCUR ON MORE THAN 2,000 ACRES ACROSS EIGHT NATIONAL PARKS IN APPALACHIA, WITH BENEFITS TO LOCAL ECONOMIES AND WATERSHED HEALTH EXTENDING BEYOND 30,000 ACRES. THIS PROJECT WILL HELP RESTORE AND MAINTAIN FORESTED ECOSYSTEMS THAT SUPPORT NATIVE PLANTS AND WILDLIFE, AS WELL AS PROVIDE CONTEXT FOR THE IMPORTANT HISTORICAL AND CULTURAL EVENTS WHICH THE NPS INTERPRETS FOR THE BENEFIT OF THE PUBLIC. OTHER BENEFICIARIES INCLUDE STATE AND LOCAL GOVERNMENTS, PUBLIC PRIVATE NONPROFIT ORGANIZATIONS, NONPROFIT INSTITUTIONS OF HIGHER EDUCATION, AND THE PUBLIC.

IN VIVO PROFILING FOR ORAL CANDIDIASIS DRUG TARGET DISCOVERY

THE FOREST STEWARDS GUILD (GUILD) WILL PARTNER WITH THE NATIONAL PARK SERVICE (NPS) TO INCREASE RESILIENCY AT SLEEPING BEAR DUNES NATIONAL LAKESHORE. THE GUILD WILL COORDINATE AND SUPPORT THE IMPLEMENTATION OF PLANNING AND RESTORATION TREATMENTS AT SEVERAL SITES ON THE NPS UNIT OVER A FIVE YEAR PERIOD. THE COMMON GOAL ACROSS ALL SITES IS TO IMPROVE FOREST RESILIENCE AND REVERSE TRAJECTORIES LEADING TO REGENERATION FAILURES OF NATIVE TREE AND SHRUB SPECIES DUE TO SOME COMBINATION OF OVERSTOCKING NON NATIVE PEST OUTBREAKS INVASIVE VEGETATION HERBIVORY AND NATURAL DISTURBANCE. SITE VISITS TACTICAL PLAN DEVELOPMENT AND ON THE GROUND TREATMENTS WILL OCCUR AS PART OF THIS AGREEMENT TO ADDRESS THESE ISSUES.THE GUILD WILL ACCOMPLISH THIS WORK THROUGH INTERNAL STAFF AND BY PARTNERING WITH LOCAL BUSINESSES. THE RESTORATION EFFORTS AT MULTIPLE SITES WILL ENHANCE FOREST RESILIENCE AT IMPORTANT NATURAL AND CULTURAL SITES HELP TRAIN NEW PRACTITIONERS AND GROW THE REGIONAL WORKFORCE TO ADDRESS THIS CRITICAL WORK.

NEIGHBORHOOD INITIAT

PHAGE-MEDIATED BIOLUMINESCENT DETECTION OF YERSINIA PESTIS

**AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** CHALLENGES IN THE PRODUCTION OF HYDROLYSATE AND OTHER MILK-DERIVED PRODUCTS CENTER ON THE EFFICIENT GENERATION OF A HIGH-YIELD PRODUCT. ENZYMES ENABLE REACTION PROCESSES TO TAKE PLACE UNDER LESS HARSH CONDITIONS THAN CHEMICAL-ONLY METHODS, INCREASE PRODUCT SPECIFICITY, AND REDUCE THE DIFFICULTY OF REACTION PROCESSING AND WASTE CLEANUP. THE USE OF ENZYMES IS NOT WITHOUT ITS OWN CHALLENGES. THE PRIMARY USE LIMITATIONS COME FROM THE COST OF THE ENZYME, AND THAT ENZYMES IN THEIR PURIFIED FORM ARE LIMITED TO A SINGLE BATCH REACTION. RECOVERY OF ENZYME FROM THE REACTION MIXTURE FOR POTENTIAL REUSE IN DAIRY PROCESSING IS DIFFICULT SINCE THE SUBSTRATES, PRODUCTS, AND THE ENZYMES ALL POSSESS SIMILAR PHYSICAL PROPERTIES. OTHER COST-DRIVERS ARE TIED TO THE NEED TO INACTIVATE THE ENZYME TO STOP A REACTION, AND REMOVAL OF THE ENZYME FROM THE FINAL PRODUCT IF THE ENZYME CAN CAUSE AN ALLERGIC OR TOXIC RESPONSE.TO ENABLE THE REUSE OF ENZYMES, STUDIES HAVE INVESTIGATED ULTRAFILTRATION MEMBRANES SCALEDTO RETAIN THE LARGER-SIZE ENZYMES AND SIMULTANEOUSLY FUNCTION AS THE FIRST PURIFICATION STEP, ORBINDING ENZYMES TO THE MEMBRANES TO COMBINE THE REACTION AND SEPARATION PROCESSES. THESE MEMBRANE-BASED SOLUTIONS DEMONSTRATED EFFICIENCY GAINS BUT SUFFERED FROM INCREASED ENZYME DEACTIVATION THROUGH SHEAR AND THERMAL STRESS, ENZYME LEAKAGE INTO THE PRODUCT, AND MEMBRANE FOULING. AS AN ALTERNATIVE, ENZYMES WERE IMMOBILIZED ON SOLID SUPPORTS SUCH AS GLASS, AGAROSE, AND SILICA. IMMOBILIZING ENZYMES TO THESE SUPPORTS ENABLED IMPROVED REGULATION OF THE REACTIONS, INCREASED ENZYME STABILITY, REDUCED PRESSURE DROP ISSUES IN THE REACTORS, IMPROVED SEPARATION OF PRODUCT FROM ENZYME, AND REDUCED CONTAMINATION. HOWEVER, THE VALUE GAINED BY THESE IMPROVEMENTS WAS OFFSET BY THE HIGH PRICE OF THE ENZYME SUPPORT MATERIAL.THE TECHNOLOGY DEMONSTRATED IN PHASE I AND TO BE ADVANCED IN THE PROPOSED PHASE II SBIR UTILIZES IMMOBIZYME, GUILD BIOSCIENCES PATENTED IMMOBILIZATION PLATFORM WHERE ACTIVE COMPONENTS, SUCH AS ENZYMES, ARE CHEMICALLY BOUND TO INACTIVE BIODEGRADABLE SUPPORT MATERIALSCONSISTING PRIMARILY OFLOW-COST OR RECYCLED BIO-WASTE. THESE RAW MATERIALS OFFER SIGNIFICANT COST SAVINGS FROM TRADITIONAL IMMOBILIZATION SUPPORTS, ENABLE HIGHER-QUALITY ENZYME PREPARATIONS, AND SIMPLIFY POST-REACTION RECYCLING/REUSE PROCESSES, WHILE RETAINING THE ADVANTAGES OF MORE EXPENSIVE SUPPORT MATERIALS.THE OVERALL AIM OF THE PHASE II PROJECT IS TO DEVELOP IMMOBIZYME INTO A MARKET-READY PRODUCT FOR WHEY AND MILK HYDROLYSIS. PHASE I EFFORTS DEMONSTRATED THAT THESE PELLETS RETAINED MORE THAN 90% OF ENZYME ACTIVITY THROUGH 350 HOURS OF CONTINUOUS REACTION WITH A PER BATCH MATERIAL LOSS OF LESS THAN 5%. PRODUCING COST-EFFECTIVE IMMOBIZYME PELLETS AT-SCALE IS ESSENTIAL TO THE SUCCESS OF THE PROJECT. IE MUST REMAIN ACTIVE AND WHOLE THROUGH MULTIPLE CONSECUTIVE BATCH REACTIONS, HAVE A SIMPLE AND EFFICIENT RECOVERY PROCESS, NOT CARRY MICROBIAL CONTAMINATION TO SUBSEQUENT REACTIONS, AND MEET REGULATORY REQUIRE,MENTS FOR CONTACT WITH MATERIALS FOR HUMAN CONSUMPTION. OUR PLAN IS TO START OPTIMIZATION BASED ON PERFORMANCE REQUIREMENTS RECOMMENDED THROUGH OUR RELATIONSHIPS WITH 4 U.S. PRODUCERS. TRANSITIONING THIS PROOF OF CONCEPT MATERIAL TO A PRODUCT WILL BE ACHIEVED BY: 1) IMPROVING THE DURABILITY OF THE PELLETS SO THE PER BATCH MATERIAL LOSS IS LESS THAN 1%, 2) DEFINING TEMPERATURE-RELATED PERFORMANCE LIMITATIONS AND OPTIMUMS, 3) DEVELOPMENT OF AN EFFECTIVE PELLET CLEANING PROCESS THAT MAXIMIZES PELLET REUSE WHILE MINIMIZING BATCH-TO-BATCH CONTAMINATION, 4) DEVELOPING A COST-EFFICIENT, UNIFORM, QUALITY, PILOT-SCALE PRODUCTION PROCESS CAPABLE OF KILOGRAM SCALE BATCHES, 5) DEMONSTRATE THE PERFORMANCE OF THE NEW MATERIAL INTERNALLY AND WITH THE SUPPORT OF EXTERNAL FACILITIES, AND 6) PROCEEDING TOWARDS REGULATORY APPROVAL FOR THE IMMOBIZYME PLATFORM. RESULTS FROM PHASE II WILL SUPPORT THE TECHNICAL AND ECONOMIC ADVANTAGES OF USING IMMOBIZYME PELLETS AS A DROP-IN REPLACEMENT FOR CURRENT WHEY ORMILK HYDROLYSIS ENZYMES.

**AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** CURRENT METHODS FOR DETECTION OF MICROBIAL CONTAMINANTS ON FOOD INDUSTRY SURFACES USE SIMPLE SWABBING/WIPING TO EXTRACT MICROBES FOR ANALYSIS. THIS REMOVES EASILY TRANSFERABLE MICROBES BUT FAILS TO CAPTUREMICROBES LIVING IN BIOFILMS, WHICH REDUCES THE DETECTION SENSITIVITY OF CURRENT FOOD SAFETY TESTSAND MAY MASK THE TRUE DEGREE OF SURFACE CONTAMINATION. THIS INCREASES THE RISK OF FOOD-BORNE OUTBREAKS THREATENING HUMAN HEALTH AND CAN RESULT IN COSTLY RECALLS FOR FOOD PROVIDERS AND LOSSES IN FOOD CUSTOMER CONFIDENCE. THE PROPOSED PROJECT WILL DEVELOP, BIOXPOSE, AN INNOVATIVE FUTURE PRODUCT THAT WILL ACT AS A DETECTION ASSAY ENHANCER AND WILL CONTAINAN OPTIMIZED SET OF ANTIBIOFILM ENZYMES THAT CAN BREAK DOWN THE PHYSICAL STRUCTURE OF BIOFILMS. BY DISSOLVING THE HOUSING PROTECTING MICROBES HIDING IN BIOFILMS THEY NO LONGER CAN RESIST COLLECTION AND DETECTION. THIS GENTLE ENZYME-CENTERED APPROACH DOES NOT KILL BIOFILMMICROBES (AS ENZYME-CONTAININGCLEANING PRODUCTS WOULD)SO THE COLLECTED MICROBES CAN BE USED IN DETECTIONASSAYS THAT REQUIRE LIVE ORGANISMS. THE USE OF BIOXPOSEWILL INCREASE THE COLLECTIONOF VIABLE MICROBES FROM BIOFILM COVEREDFOOD PROCESSING SURFACES ALLOWINGINCREASEDDETECTION OF SURFACE CONTAMINATION WITHALL CURRENT TYPES OF FOOD SAFETY DETECTION ASSAYS. THIS UNIVERSAL APPLICATION WILL ALLOW FOOD SAFETY MANAGERS TO CONTINUE USING THEIR PREFERRED DETECTION SYSTEMS BUT WITH BETTERPERFORMANCE, AND ONLY A MINOR INCREASEIN COST.THE PHASE I RESULTS PROVIDED SEVERAL CANDIDATE ENZYME COCKTAILS (ECS) WITH HIGH ACTIVITY AGAINST A MULTI-SPECIES BIOFILM MODEL. THE PHASE II PROJECT WILL FURTHER DEVELOP THE TOP PHI ECS TO REACH A MARKET-READY PRODUCT, BIOXPOSE, AND WILL CONSIST OF THREE OBJECTIVES. IN TECHNICAL OBJECTIVE 1, REFINEMENTS TO THE BIOFILM ASSAY AND BACTERIAL RELEASE ASSAY WILL BE INSTITUTED AND TOP ECS WILL BE MORE COMPREHENSIVELY TESTED. FOR TECHNICAL OBJECTIVE 2, LEAD ECS WILL BE CONVERTED INTO PRODUCT READY FORMULATIONS THAT ACHIEVES THE MICROBE RELEASE PERFORMANCE DESIRED IN THE FINAL PRODUCT. IN TECHNICAL OBJECTIVE 3, THE TOP EC WILL BE TESTED AGAINST A MORE ADVANCED MULTI-SPECIES BIOFILM USING 2 DOWNSTREAM DETECTION ASSAYS TO COMPARE BIOXPOSE-TREATED VS CONTROLS BY AN INDEPENDENT CONTRACT LAB.WITH THE SUCCESSFUL DEVELOPMENT OF BIOXPOSE, FOOD INDUSTRY SURFACE CONTAMINATION MONITORING WILL ACHIEVE IMPROVED SENSITIVITY, ACCURACY, AND RELIABILITY THROUGH THE USE OF BIOXPOSE, A DETECTION ASSAY ENHANCER, WHICH WILL PROVIDE BETTER PROTECTION FROM FOOD-BORNE ILLNESSES, OUTBREAKS,AND WILL REDUCE PRODUCT RECALLS, AND DROPS IN CONSUMER CONFIDENCE.

FY 2020 HEALTH CENTER PROGRAM LOOK-ALIKES: EXPANDING CAPACITY FOR CORONAVIRUS TESTING

PROGRAMMING SUPPORTS A COMPREHENSIVE NETWORK OF COOPERATIVE STUDY UNITS TO FACILITATE COOPERATIVE RESEARCH, TECHNICAL ASSISTANCE, EDUCATION, AND TRAINING TO INFORM SCIENCE-BASED MANAGEMENT OF THE NATIONAL PARK SYSTEM. THE STUDY UNITS FACILITATE MULTI-DISCIPLINARY SCIENCE AND INTEGRATED INFORMATION PRODUCTS ACROSS MULTIPLE GEOGRAPHIC REGIONS OF THE NATIONAL PARK SYSTEM. AS A RESULT OF THIS BIPARTISAN INFRASTRUCTURE LAW-ECOSYSTEM RESTORATION PROJECT, FOREST RESTORATION WILL OCCUR ON UP TO 1,000 ACRES OF HEMLOCK RAVINE FORESTS WITHIN THE DELAWARE WATER GAP NATIONAL RECREATION AREA. HEMLOCK RAVINES ARE POPULAR RECREATION AREAS IN THE PARK, IDENTIFIED IN THEIR FOUNDATION DOCUMENT AS FUNDAMENTAL RESOURCES THAT PROVIDE STUNNING SCENIC RESOURCES AND DISTINCT AESTHETIC VALUES. BENEFICIARIES INCLUDE STATE AND LOCAL GOVERNMENTS, FEDERALLY RECOGNIZED INDIAN TRIBAL GOVERNMENTS, PUBLIC PRIVATE NONPROFIT ORGANIZATIONS, NONPROFIT INSTITUTIONS OF HIGHER EDUCATION, AND THE GENERAL PUBLIC.

AFCEC SEASONAL WILDLAND FIRE SUPPORT

HEALTH CARE AND OTHER FACILITIES

CONTINUUM OF CARE PROGRAM

IMMOBILIZED ENZYME-BASED BURN WOUND DEBRIDEMENT FOR APPLICATION AT THE POINT OF INJURY AND PROLONGED FIELD CARE

BIOLUMINESCENT REPORTER PHAGE FOR THE DIAGNOSTIC DETECTION OF SHIGELLOSIS

EXPAND MEDIA FREEDOM IN KENYA BY PROVIDING TRAINING FOR JOURNALISTS, PROMOTING QUALITY AND ETHICAL JOURNALISM

OPTIMIZED METHODS FOR ISOLATION AND CHARACTERIZATION OF BACTERIOPHAGE BY VT-FACS - ABSTRACT ANTIBIOTIC-RESISTANT (AMR) PATHOGENS ARE EMERGING AT ALARMING RATES AND CURRENT TREATMENT OPTIONS ARE BECOMING INCREASINGLY LIMITED, EXPENSIVE, AND IN SOME CASES, NONEXISTENT. TWO MILLION ANTIBIOTIC RESISTANT INFECTIONS OCCUR ANNUALLY IN THE U.S AND, ALTHOUGH THERE IS AN URGENT AND IMMEDIATE NEED FOR AGENTS WITH ACTIVITY AGAINST THESE EMERGING MULTIDRUG-RESISTANT PATHOGENS, ONLY 2 NEW ANTIBIOTICS HAVE BEEN APPROVED SINCE 2009. THEREFORE, THE DEVELOPMENT OF ALTERNATIVE ANTIBACTERIAL AGENTS IS CRUCIAL. A PROMISING ALTERNATIVE TO ANTIBIOTICS IS BACTERIOPHAGE (PHAGE) WHICH HAVE A NATURAL ABILITY TO SELECTIVELY INFECT AND KILL TARGET BACTERIA. PHAGE IS RECEIVING RENEWED INTEREST AS A SAFE AND EFFECTIVE THERAPY, BUT TECHNIQUES FOR THE ISOLATION, CHARACTERIZATION, PRODUCTION AND TESTING OF PHAGES TO IDENTIFY ‘SUITABLE’ PHAGE COCKTAIL CANDIDATES ARE TIME CONSUMING, EXPENSIVE AND LOW THROUGHPUT. HERE WE PROPOSE INNOVATIVE HIGH-THROUGHPUT METHODOLOGIES BASED ON A PROVEN VT-FACS PLATFORM WHICH MITIGATE OR CIRCUMVENT THESE CONSTRAINTS PROVIDING TIME- AND COST-SAVINGS IN DEVELOPMENT AND PRODUCTION OF THERAPEUTIC PHAGE COCKTAILS. THE GOAL OF THIS PROJECT IS FURTHER DEVELOPMENT OF VT-FACS, A HIGH-THROUGHPUT PHAGE ISOLATION PIPELINE, TO ADVANCE, CHARACTERIZE AND SUPPORT THE USE AND RAPID SELECTION OF PHAGE AS AN ALTERNATIVE TO CLASSICAL ANTIBIOTICS. AIM 1 WILL DEFINE AND VALIDATE METHODS FOR SCREENING AND PREDICTING LIKELIHOOD OF INSOLATING PATHOGEN- SPECIFIC PHAGE IN PREPARED AND ARCHIVED LYSATES FOR INFORMED ON-DEMAND SELECTION. IN AIM 2 WE WILL USE VT- FACS AND ADAPT CURRENT WORKFLOW TO IDENTIFY THE OPTIMAL BACTERIAL HOST FOR PRODUCTION OF THE MOST INCLUSIVE AND HIGHEST TITER PHAGE TO MINIMIZE FINAL COCKTAIL COMPLEXITY AND PRODUCTION COST. FINALLY, AIM 3 WILL USE VT-FACS TO DEVELOP AND VALIDATE HIGH-THROUGHPUT METHODS THAT ISOLATE PHAGE WITH ANTIBIOFILM ACTIVITY. THROUGH THESE 3 INDEPENDENT AIMS WE WILL DEVELOP ESSENTIAL METHODS WHICH EACH ACCELERATE DEVELOPMENT AND PRODUCTION OF BACTERIOPHAGE AS AN ANTIBACTERIAL THERAPY. FOR HIGHEST THROUGHPUT AND EFFICIENCY IN A FUTURE WORKING ENVIRONMENT, THE PROPOSED METHODS CAN BE APPLIED IN TANDEM, ARE INHERENTLY SCALABLE AND HAVE POTENTIAL TO BE AUTOMATED. SUCCESSFUL COMPLETION OF THIS WORK WILL PROVIDE EXPEDITED AND COST-EFFECTIVE MEANS TO SELECT AND PRODUCE PATHOGEN SPECIFIC PHAGE WITH THE REQUIRED HOST-RANGE AND SECONDARY ACTIVITIES NECESSARY FOR PHAGE THERAPY. THE ULTIMATE OUTCOME OF THE RESEARCH SUPPORTS IMPROVED AND PERSONALIZED PHAGE THERAPY OPTIONS TO COMBAT TODAY’S URGENT ANTIBIOTIC RESISTANCE THREAT.

CFRP-RIO TRAMPAS WATERSHED MULTI-JURISDICTIONAL FOREST RESTORATION PLANNING

CFRP-CAPACITY BUILDING, RESTORATION AND WOOD UTILIZATION IN THE BLUEWATER WATERSHED

RIO DE LAS TRAMPAS WATERSHED RESTORATION PHASE I: STATE TRUST LANDS

IMPLEMENTATION: CERRO DEL AIRE

SUPPORTING THE RIO DE LAS TRAMPAS FOREST COUNCIL’S LEÑERO PROGRAM

PHAGE MEDIATED DETECTION OF BACILLUS ANTHRACIS ON DELIBERATELY CONTAMINATED FRESH FOODS

FY 2023 EXPANDING COVID-19 VACCINATION

PERAGAMANA GUILD

SVPP

**AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** BUILDING ON MOMENTUM FROM USDA BFRDP #2016-03316, THIS FARMERS-TO-FARMLAND PROJECT USES A MULTI-PRONGED APPROACH TO FACILITATE ENTRY INTO FARMING FOR BEGINNING FARMERS AND RANCHERS (BFRS), WITH A FOCUS ON WOMEN AND THE LATINX COMMUNITY, IN THE REMOTE SAN JUAN ISLANDS, WASHINGTON. THE SAN JUANS ARE A TOP TOURIST DESTINATION, WHICH HAS RESULTED IN HIGH LAND PRICES BUT ALSO ROBUST MARKETS FOR LOCAL FOOD. OUR COLLABORATIVE APPROACH WILL MEET BFRS WHERE THEY ARE IN DEVELOPING FARMING GOALS, SKILLS, AND ACCESS TO LAND. WITH SUPPORT FROM AGENCIES AND PEERS, WE WILL OFFER BFRS TECHNICAL ASSISTANCE, EDUCATION, AND MENTORING, AS WELL AS HELP NAVIGATING MARKETS AND CONNECTING TO FARMLAND. OUR FIRST BFRDP PROJECT SHOWED LACK OF BFR EXPERIENCE AS A BARRIER TO LAND ACCESS. HERE, WE WILL DEVELOP A MODEL FOR LAND SHARING AND COLLABORATIVE LAND MANAGEMENT TO JUMP-START BFRS THROUGH A SUPPORT SYSTEM THAT DRAWS UPON THE EXPERIENCE AND RESOURCES OF PROJECT COLLABORATORS AND SYNCS WITH OTHER BFR SERVICES.THE AVERAGE AGE OF SAN JUAN COUNTY (SJC) FARMERS IS 60.3, COMPARED TO THE 57.5 NATIONAL AVERAGE (USDA AG CENSUS 2017). FOR FARMING TO CONTINUE IN THE ISLANDS, WE MUST ACTIVELY CULTIVATE OUR NEXT GENERATION OF FARMERS. WITH THIS PROJECT, WE WILL SERVE 218 UNIQUE BFRS, OF WHICH 35% WILL BE WOMEN AND 5% LATINX.

PHAGE-ACCELERATED TEST SYSTEM FOR ID/AST OF URINARY TRACT INFECTIONS

BUILDING A FOREST STEWARDSHIP WORKFORCE IN VIRGINIAS CENTRAL APPALACHIANS

G&A CFRP 14-18 - BUILDING CAPACITY FOR RX BURNS IN THE ZUNI MTNS

CONTINUUM OF CARE PROGRAM

CONTINUUM OF CARE PROGRAM

SUPPORTIVE HOUSING NEW

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WOMEN OWNING WOODLANDS

PROVIDING OPPORTUNITIES FOR WORK, EDUCATION AND RECREATION (POWER-UP) FOR DISCONNECTED AND AT-RISK YOUTH

WE PROPOSE A SCIENCE MANAGEMENT PARTNERSHIP THAT WILL IDENTIFY NEW ADAPTIVE MANAGEMENT STRATEGIES FOR THE 232 PARTNERSHIP A LANDSCAPE SCALE COLLABORATIVE GROUP THAT WORKS TOGETHER TO PRIORITIZE PLAN IMPLEMENT AND MONITOR WORK IN A NEARLY 5 MILLION ACRE GEOGRAPHY IN NORTHERN NEW MEXICO AND SOUTHERN COLORADO. WORKING CLOSELY WITH MANAGERS RESEARCHERS PRACTITIONERS AND OTHERS IN THE PARTNERSHIP E.G. THE 232 EXECUTIVE COMMITTEE USFS LINE OFFICERS BOARD OF FOREST SUPERVISORS WE WILL IDENTIFY ECOSYSTEM VULNERABILITIES REVIEW EXISTING TOOLS TO MODEL FUTURE CLIMATE AND DEVELOP STRATEGIES USING THOSE TOOLS TO RESPOND TO FUTURE THREATS.

LANDSCAPE SCALE RESTORATION SUSTAINING ASH OF THE NORTHEAST THROUGH TRAINING TREATMENT AND OUTREACH

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SAN JUAN ISLANDS FOOD HUB: COORDINATING SALES AND DISTRIBUTIN OF FARM PRODUCTS IN THE SAN JUAN ISLANDS

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NOVEL MULTICOMPONENT PROTEIN COMPLEX CONTAINING BOTH HYDROLYTIC AND OXIDATIVE ENZYMES CAPABLE OF DECONSTRUCTING/DEGRADING LIGNOCELLULOSE

THIS AGREEMENT IS TO REDUCE THE RISK AND IMPACT OF FIRES IN NEW MEXICO. THIS INCLUDES EDUCATION AND OUTREACH PROGRAMS. THE PUBLIC WILL BENEFIT THROUGH A COORDINATED EFFORT TO ADDRESS WILD FIRES.

MULTIFAMILY HOUSING SERVICE COORDINATORS

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AMERICAN RESCUE PLAN

AFFINITY REAGENTS FOR THE DETECTION OF MODIFIED RNAS AND TRANSCRIPTOME-WIDE MAPPING OF RNA MODIFICATIONS

CONTINUUM OF CARE PROGRAM

JFSP 11-1-3-10

RURAL HEALTH CLINIC VACCINE CONFIDENCE PROGRAM

CONTINUUM OF CARE PROGRAM

PUBLIC CHARTER SCHOOLS

CF CONGRESSIONALLY DIRECTED GRANTS

INCREASING RESILIENCY IN SOUTHERN NEW ENGLAND OAK FORESTS

CONTINUUM OF CARE PROGRAM

THE NASA INNOVATIVE ADVANCED CONCEPTS (NIAC) PROGRAM FOCUSES ON EARLY-STAGE FEASIBILITY STUDIES OF VISIONARY CONCEPTS THAT ADDRESS NATIONAL GOVERNMENT AND COMMERCIAL AEROSPACE GOALS. CONCEPTS ARE SOLICITED FROM ANY FIELD OF STUDY THAT OFFERS A RADICALLY DIFFERENT APPROACH OR DISRUPTIVE INNOVATION THAT MAY SIGNIFICANTLY ENHANCE OR ENABLE NEW HUMAN OR ROBOTIC SCIENCE AND EXPLORATION MISSIONS. THE NIAC PROGRAM SEEKS EXCITING UNEXPLORED CREDIBLE AEROSPACE CONCEPTS WITH AT LEAST ONE MISSION APPLICATION THAT ADDRESSES NASA GOALS OR OFFERS WIDER BENEFITS WITH SPACE OR AERONAUTICS APPLICATIONS. NIAC FOCUSES ON EARLY STUDIES OF VISIONARY AEROSPACE CONCEPTS. THESE AEROSPACE ARCHITECTURES PROPOSED IN THE CONTEXT OF A REPRESENTATIVE MISSION WILL GENERALLY ADVANCE FROM TECHNOLOGY READINESS LEVEL (TRL) 1-2 IN MATURITY AND ARE AIMED TEN OR MORE YEARS IN THE FUTURE.

BIOGAS PRODUCTION FROM WASTE UTILIZES A BROAD RANGE OF FEEDSTOCKS, FROM AGRICULTURAL WASTE TO METROPOLITAN SEWAGE. EFFECTIVE UTILIZATION OF THE AVAILABLE FEEDSTOCK IS NECESSARY TO MAINTAIN THE ECONOMIC VIABILITY AND OVERALL SUSTAINABILITY OF THE DIGESTION. THE ADDITION OF ENZYMES HAS BEEN INVESTIGATED TO IMPROVE DIGESTION OF DIFFICULT MATERIALS SUCH AS FATS AND PROTEINS WHICHINCREASESBIOGAS PRODUCTION, REDUCESTOTAL SOLIDS AT THE CONCLUSION OF DIGESTION, AND ACTIVELY MANAGES ISSUES WITH FOAMING. RECENT STUDIES HAVE DETERMINED THAT ENZYMATIC TREATMENT OF ANAEROBIC DIGESTER FEED MATERIALS CAN INCREASE THE QUALITY AND QUANTITY OF BIOGAS GENERATION WITHOUT THE NEED FOR ADDITIONAL ENERGY INPUTS. HOWEVER, THE UTILITY OF ENZYME SUPPLEMENTS IS LIMITED AS THE ENZYMES ARE RAPIDLY DEGRADED IN THE MICROBIALLY ACTIVE ENVIRONMENT OF THE DIGESTER. GUILD BIOSCIENCES' PATENTED ENZYME IMMOBILIZATION TECHNOLOGY, IMMOBIZYME™, CAN PROVIDE AN ECONOMIC AND PRACTICAL SOLUTION TO IMPROVING THE SURVIVABILITY OF ENZYME INDIGESTER APPLICATIONS. IMMOBILIZED ENZYMES HAVE DEMONSTRATED SIGNIFICANTLY IMPROVED STABILITY AND REACTIVITY IN AGGRESSIVE, DIGESTIVE, OR DENATURING ENVIRONMENTS. THIS COMBINED WITHTHE LOW MANUFACTURING COST OF THE IMMOBIZYME PROCESS SIGNIFIES THAT AN ENZYMATIC SOLUTION TO IMPROVING DIGESTER PERFORMANCEIS POSSIBLE.THE AIM OF THIS PROJECT IS TO EVALUATE THE CAPABILITY OF MULTIPLE ENZYMES, FOLLOWING IMMOBILIZATION THROUGH OUR PATENTED TECHNOLOGY, TO INCREASE THE PRODUCTION OF BIOGAS OF AN ANEROBIC DIGESTER CONSISTENTLY AND SIGNIFICANTLY OVER THE COURSE OF A 28-DAY BENCH-SCALE TEST. RESULTS FROM PHASE I WILL IDENTIFY THE BEST ENZYME CANDIDATES AND IMMOBILIZATION FORMULATIONS FOR A PILOT-SCALE STUDY BASED ON: 1) OVERALL BIOGAS GENERATION, 2) ACTIVE LIFETIME OF THE IMMOBILIZED ENZYME IN THE REACTOR, AND 3) QUANTITY OF ENZYME REQUIRED.

HEALTH CARE AND OTHER FACILITIES

MOLECULARLY SELF-ASSEMBLED MULTI-FUNCTIONAL DNA ORIGAMI NANOSTRUCTURE FOR SAFE AND EFFECTIVE SIRNA DELIVERY

A NOVEL ANTISENSE THERAPEUTIC FOR TREATMENT OF ASPERGILLUS FUMIGATUS INFECTIONS

THE MULTI-USE FOOD CENTER PLANNING PROJECT AIMS TO INCREASE ECONOMIC OPPORTUNITIES FOR FARMERS AND VALUE-ADDED PRODUCERS IN SAN JUAN COUNTY, WASHINGTON BY EXPANDING DIRECT PRODUCER-TO-CONSUMER MARKETS, ORGANIZATIONAL AND INFRASTRUCTURE CAPACITY, AND ACCESS TO HEALTHY LOCAL FOOD. ISLAND FARMERS AND PRODUCERS FACE BARRIERS IN THE GEOGRAPHICALLY ISOLATED COUNTY (SERVED BY THE WASHINGTON STATE FERRY SYSTEM), INCLUDING LIMITED ACCESS TO MARKETS AND A LACK OF ADEQUATE PROCESSING AND STORAGE FACILITIES. HOWEVER, THERE IS SIGNIFICANT DEMAND FOR LOCAL PRODUCTS, DRIVEN BY BOTH COMMUNITY, INSTITUTIONAL, AND TOURIST CONSUMERS, AS WELL AS ADDITIONAL POTENTIAL FOR AGRITOURISM. THIS COUNTY-WIDE ENDEAVOR IS LED BY NONPROFITS, THE LOPEZ ISLAND FAMILY RESOURCE CENTER AND TAPROOT COMMUNITY KITCHEN, WORKING IN COLLABORATION WITH THE SAN JUAN ISLANDS FOOD HUB, A MULTI-STAKEHOLDER COOPERATIVE. THE LFPP GRANT WOULD ENABLE THIS PUBLIC-PRIVATE PARTNERSHIP (PPP) TO 1) DEVELOP A BUSINESS AND MARKETING PLAN TO DETERMINE AN OWNERSHIP MODEL, A FINANCIAL STABILITY STRATEGY, AND AN OPERATIONS PLAN; 2) IDENTIFY KEY ELEMENTS FOR THE DESIGN OF THE FACILITY; AND, 3) INCREASE KNOWLEDGE OF WHOLESALE AND RETAIL FOOD SAFETY REGULATION AND BEST PRACTICES AMONG FACILITY USERS. THE MULTI-USE FOOD CENTER WOULD SERVE AS A REPLICABLE PILOT PROJECT TO DEVELOP MID-TIER VALUE CHAINS AMONG THE COUNTY'S ISLANDS AND WITH NEIGHBORING COUNTIES ON THE MAINLAND BY PROVIDING ACCESS TO PROCESSING AND VALUE-ADDED PRODUCTION, DRY/COLD/FROZEN STORAGE, AND EDUCATIONAL/INCUBATOR SPACE FOR USE BY BOTH NON-PROFITS AND ENTREPRENEURS WHO BUY DIRECTLY FROM LOCAL FARMERS.

WOMEN OWNING WOODLANDS

ASSISTANCE TO FIREFIGHTERS GRANT

MAINE-MASSABESIC REGION COMMUNITY WILDFIRE PROTECTION PLAN AND WILDFIRE RISK REDUCTION

PURPOSE: TO SUPPORT PERSONNEL EXPENSES IN RESPONSE TO AND RECOVERY FROM THE COVID-19 PANDEMIC.

SBIR PHASE I: CATALYST FOR THE OXIDATION OF METHANE IN COAL MINE VENTILATION AIR

SBIR PHASE I: BIOLUMINESCENT REPORTER PHAGE FOR THE RAPID AND SPECIFIC DETECTION OF THE CAUSATIVE AGENT OF BACTERIAL BLIGHT

EDI SPECIAL PROJECTS

CHALLENGES IN THE PRODUCTION OF HYDROLYSATE AND OTHER MILK-DERIVED PRODUCTS CENTER ON THE EFFICIENT GENERATION OF A HIGH-YIELD PRODUCT. ENZYMES ENABLE REACTION PROCESSES TO TAKE PLACE UNDER LESS HARSH CONDITIONS THAN CHEMICAL-ONLY METHODS, INCREASE PRODUCT SPECIFICITY, AND REDUCE THE DIFFICULTY OF REACTION PROCESSING AND WASTE CLEANUP. THE USE OF ENZYMES IS NOT WITHOUT ITS OWN CHALLENGES. THE PRIMARY USE LIMITATION IS THE COST OF THE ENZYME, AS ENZYMES ARE REFINED AND PURIFIED PROTEIN PRODUCTS. LOWER-COST ENZYME OPTIONS ARE AVAILABLE, BUT TYPICALLY SACRIFICE PURITY AND HAVE BROADER BATCH-TO-BATCH VARIABILITY AS A TRADEOFF TO COST. THE OTHER SIGNIFICANT LIMITATION IS THAT ENZYMES IN THEIR PURIFIED FORM ARE LIMITED TO A SINGLE BATCH REACTION. RECOVERY OF ENZYME FROM THE REACTION MIXTURE FOR POTENTIAL REUSE IS DIFFICULT SINCE THE SUBSTRATES, PRODUCTS, AND THE ENZYMES ALL POSSESS SIMILAR PHYSICAL PROPERTIES. OTHER COST-DRIVERS ARE TIED TO THE NEED TO INACTIVATE THE ENZYME TO STOP A REACTION, AND REMOVAL OF THE ENZYME FROM THE PRODUCT IF IT MAY CAUSE AN ALLERGIC OR TOXIC RESPONSE.TO ENABLE THE REUSE OF SOLUBLE ENZYMES, STUDIES HAVE INVESTIGATED ULTRAFILTRATION MEMBRANES SCALED TO RETAIN THE LARGER-SIZE ENZYMES AND SIMULTANEOUSLY ACT AS THE FIRST PURIFICATION STEP AND BINDING ENZYMES TO AMEMBRANE TO COMBINE THE REACTION AND SEPARATION PROCESSES. THESE MEMBRANE-BASED SOLUTIONS DEMONSTRATED EFFICIENCY GAINS, BUT SUFFERED FROM INCREASED ENZYME DEACTIVATION THROUGH SHEAR AND THERMAL STRESS, ENZYME LEAKAGE INTO THE PRODUCT, AND MEMBRANE FOULING. AS AN ALTERNATIVE, ENZYMES WERE IMMOBILIZED ON SOLID SUPPORTS SUCH AS GLASS, AGAROSE, AND SILICA. IMMOBILIZING ENZYMES TO THESE SUPPORTS PERMITTED IMPROVED REGULATION OF THE REACTIONS, INCREASED ENZYME STABILITY, REDUCED PRESSURE DROP ISSUES IN THE REACTORS, IMPROVED SEPARATION OF PRODUCT FROM ENZYME, AND REDUCED CONTAMINATION. HOWEVER, THE VALUE GAINED BY THESE IMPROVEMENTS WAS OFFSET BY THE HIGH COST OF THE SUPPORT MATERIAL.THE TECHNOLOGY DEVELOPED ON THIS SBIR SIMILARLY SEEKS TO REDUCE ENZYME-RELATED OPERATIONAL COSTS THROUGH IMMOBILIZATION OF ENZYMES. THIS EFFORT WILL FOCUS ON BEAD-LIKE PELLETS MADE FROM LOW-COST AND RECYCLED BIO-WASTE MATERIALS AS THE ENZYME SUPPORT. USING THESE ROBUST AND LOW-COST SUPPORT MATERIALS CAN ENABLE THE USE OF HIGHER-QUALITY ENZYME PREPARATIONS AND LEADS TO MORE UNIFORM PRODUCTS AND SIMPLIFIED POST-REACTION PROCESSES. SIMILARLY, THE PELLETS RETAIN THE ENZYME SO STOPPING THE REACTION OR SEPARATING THEM FROM THE REACTOR INVOLVES SIMPLY FILTERING THEM FROM THE REACTION SOLUTION. EXISTING PLANTS CAN BE ADAPTED THROUGH USING ABOLT-ON COLLECTION SYSTEM. THE PHASE I GOAL IS TOVALIDATE THE CAPABILITY OF OUR PATENTED IMMOBIZYME™ TECHNOLOGY AS A SOLUTION FOR THE TWO PRIMARY ENZYME-BASED REACTIONS USED BY WHEY PROCESSORS: PROTEIN HYDROLYSIS AND LACTOSE REMOVAL. DATA FROM THIS EFFORT WILL DEMONSTRATE THAT THIS TECHNOLOGY IS EASILY APPLIED TO WHEY PROCESSING AND WILL BENEFICIALLY SUPPORT THE NEEDED ENZYME REACTIONS.

CONTINUUM OF CARE PROGRAM

ECOSYSTEM PROCESS RESTORATION THROUGH PRESCRIBED FIRE BUILDING IN BLACK LAKE

PHAGE-MEDIATED BIOLUMINESCENT DETECTION OF YERSINIA PESTIS.

READINESS AND EMERGENCY MANAGEMENT FOR SCHOOLS

THE RECIPIENT WILL USE THESE FUNDS FOR THE PROJECT "CAPACITY BUILDING OF MARGINALIZED WOMEN"

IMMOBIZYME PLATFORM FOR ENZYME RECYCLING IN BIOETHANOL PRODUCTION

TO SUPPORT THE IMPLEMENTATION OF THE NATIONAL BLUEPRINT FOR CREATIVE YOUTH DEVELOPMENT A COLLECTIVE IMPACT PROJECT.

TO SUPPORT THE IMPLEMENTATION OF THE NATIONAL BLUEPRINT FOR CREATIVE YOUTH DEVELOPMENT A COLLECTIVE IMPACT PROJECT.

TO SUPPORT A NATIONWIDE COLLECTIVE IMPACT INITIATIVE AND THE CREATION OF A CREATIVE YOUTH DEVELOPMENT BLUEPRINT.

TO SUPPORT THE OPERA-BASED TEACHING AND LEARNING EDUCATION PROGRAM IN PUBLIC ELEMENTARY SCHOOLS IN NEW YORK CITY AND NEW JERSEY.

DEVELOPMENT OF AN ANTISENSE-BASED ANTIMICROBIAL AGENT FOR TREATMENT OF PHYTOPHTHORA CAPSICI

RURAL BUSINESS ENTERPRISE GRANTS

FOOD BORNE INFECTIONSFROM PATHOGENS ORIGINATING FROMFOOD PROCESSING CONTINUE TO BE A PROBLEM FOR THE FOOD INDUSTRY RESULTING IN SICKNESS, DEATH, AND SUBSTANTIAL FINANCIAL LOSSES. CURRENT CONTAMINATION MONITORING TESTS USED ON FOOD INDUSTRY SURFACES UTILIZESWABBING/WIPING METHODS TO COLLECTMATERIAL TO TEST FOR THE PRESENCE OF PATHOGENIC MICROORGANISMS ON THESE SURFACES. THIS TYPE OF COLLECTION CAPTURESEASILY TRANSFERABLE PATHOGENS,BUT FAILS TO COLLECTMICROBESENTRENCHED IN HARDENED BIOLOGICAL SURFACE COMMUNITIES CALLED BIOFILMS THAT ARE PROTECTED FROMCLEANING AND DISINFECTION PROCESSES BUT CAN STILL CONTAMINATE FOOD. THE FAILURETO COLLECT THESE TIGHTLY BOUND SURFACE BACTERIA REDUCES THE SENSITIVITY OF CONTAMINATION MONITORING TESTSAND CANMASK THE TRUE DEGREE OF CONTAMINATION (AS MOST BIOFILM OCCUPANTS REMAIN ON THE SURFACE NOT ON THE SWAB/WIPE.AND NEVER REACH THE TEST TO BE DETECTED).THE PROPOSED PROJECT WILL DEVELOP, BIOXPOSE, AN INNOVATIVE ENZYME-BASED PRODUCT CONCEPT THAT WILL BE A LIQUID CONTAINING SEVERAL DIFFERENT ENZYMES THAT COLLECTIVELY DISINTEGRATETHE WEB-LIKE SUPPORT STRUCTURE OF BIOFILMS RELEASINGLIVEBACTERIA FROM THE SAMPLEDSURFACESSO THEY CAN BE EFFICIENTLY COLLECTED AND DETECTED BY CURRENTAND FUTURE CONTAMINATION MONITORING TESTS. USING BIOXPOSEWILL MAKETHESETESTS MORE SENSITIVE AND ACCURATE WHICH WILL PROVIDE SAFER FOOD INDUSTRY FACILITIES AND WILL REDUCE FOOD BORNE ILLNESSES.TO ACHIEVE THIS, THE PROJECT WILL DEVELOP A TEST SYSTEM ( ANTIBIOFILM ASSAY) SPECIFICALLY ULITIZING PATHOGENIC BACTERIA AND NON-PATHOGENIC RESIDENTBACTERIA COMMONLY COLLECTED FROMFOOD INDUSTRY SURFACES (AS OPPOSED TO USING GENERIC PATHOGENIC BACTERIA). ONCE AN ANTIBIOFILM ASSAY THAT WORKS WITHALL 8 BACTERIA INTHE PROJECTIS DEVELOPED,ASET OF 13 ENZYMES WELL RECOGNIZED FOR THEIR ABILITY TO BREAK DOWN BIOFILMS WILL BE COMBINED IN PAIRSAND TESTED IN ANTIBIOFILM ASSAYS WITH BIOFILMS MADE BYEACH OF THE BACTERIA AND THE TOP 6 SELECTED FOR FURTHER WORK. SINGLE SPECIES ANTIBIOFILM ASSAYS ARE THE NORM IN BIOFILM RESEARCH, BUT ARE SIMPLE TEST SYSTEMS THAT DON'T CLOSELY REPRESENT NATURAL BIOFILMS, WHICH USUALLY HAVE A NUMBER OF DIFFERENT BACTERIA PRESENT. TO PROVIDE A MORE CHALLENGING AND REALISTIC TEST SYSTEM, A SECONDRESEARCH THREAD WILL DEVELOPANTIBIOFILM ASSAYS COMPOSED OF 4 DIFFERENTBACTERIAL SPECIES (A MULTI-SPECIES ANTIBIOFILM ASSAY) GROWN ON EITHER PLASTIC OR STAINLESS STEEL SURFACES (TWO VERY COMMON SURFACE TYPES IN THE FOOD INDUSTRY) WILL BEMORE ROBUST AND WILL BE MORERESISTANCETOTYPICAL AGENTS USED FOR CLEANING/DISINFECTING FOOD INDUSTRYSURFACES. THE MULTI-SPECIES ANTIBIOFILM ASSAYS DEVELOPEDWILL BE USED TO EVALUATE THE 6 TOP PERFORMING2-ENZYME COMBINATIONS IDENTIFIED IN THE SINGLE SPECIES ANTIBIOFILM ASSAYS, AND THENEVEN MORE EFFECTIVE 3 ENZYME CANDIDATE COCKTAILS WILL BE EVALUATED WITH THESEMORE REALISTIC ASSAYS. FINALLY, TO FURTHER APPROACH REAL LIFE TEST CONDITIONS,A COMMERCIAL ATP ASSAY THAT IS WIDELYUSED IN THE FOOD INDUSTRY TO TEST FOR SURFACE CONTAMINATION AND BIOFOULING WILL BE USED TO EVALUATE HOW MUCH IMPROVEMENT IN PERFORMANCE THEDEVELOPED ENZYME COCKTAILS PROVIDEOVER THE STANDARD APPLICATIONOF THE COMMERCIAL SYSTEM.WE EXPECT THAT THIS PROJECT WILL PROVIDE SOLID EVIDENCE OF THE FEASIBILITY OF THESTRATEGY OF USING ENZYME COCKTAILSTO LIBERATE VIABLE BACTERIA FROM BIOFILMS SO THEY CAN BE COLLECTED AND ANAYZED BY CURRENT SURFACE CONTAMINATION TEST SYSTEMS. IT IS THEN EXPECTED THAT MORE RIGOROUS AND COMPREHENSIVE TESTING AND FORMULATION DEVELOPMENT EFFORTSWILL BE ACCOMPLISHED INA LARGER SBIR PHASE II PROJECT. THISEFFORT WILL RESULT IN A PROTOTYPE ENZYME FORMULATION THAT WILL BE DEVELOPED INTO ACOMMERCIAL PRODUCT,BIOXPOSE, WHICH WILL BE USED BY FOOD SAFETY MANAGERS TO IMPROVE THESENSITIVITY, ACCURACY, AND VALUE OF CURRENT AND FUTURE SURFACE CONTAMINATION TESTS INCREASING FOOD INDUSTRY SAFETY AND REDUCING FOOD BORNE INFECTIONS IN THE US AND BEYOND.

ASSISTANCE TO FIREFIGHTERS GRANT PROGRAM

TO SUPPORT PHASE II OF THE OPERA INSTITUTE PROGRAM.

PURPOSE: THE FAMILY SELF-SUFFICIENCY PROGRAM (FSS) IS GOVERNED BY SECTION 23 OF THE 1937 HOUSING ACT. THE PURPOSE OF FSS AWARDS IS TO FUND THE SALARIES AND FRINGE FOR FSS COORDINATORS, WHO ARE RESPONSIBLE FOR IMPLEMENTING FSS FOR RESIDENTS OF PUBLIC HOUSING, HOUSING CHOICE VOUCHERS (HCV), AND PROJECT-BASED RENTAL ASSISTANCE (PBRA) PROPERTIES. FSS FUNDS APPROXIMATELY 900 PROGRAMS RUN BY PUBLIC HOUSING AUTHORITIES (PHAS) AND PBRA PROPERTY OWNERS ACROSS THE COUNTRY. RENEWAL APPLICANTS ARE PRIORITIZED BY STATUTE.; ACTIVITIES TO BE PERFORMED: FUNDS UNDER FSS GRANTS ARE USED SOLELY TO FUND SALARY AND FRINGE BENEFITS FOR FSS COORDINATORS. RESPONSIBILITIES OF FSS COORDINATORS UNDER THE AWARD INCLUDE, BUT ARE NOT LIMITED TO, ENROLLING, AND MAINTAINING FAMILIES IN THE PROGRAM, WORKING WITH FAMILIES TO PREPARE INDIVIDUAL TRAINING AND SERVICES PLANS (ITSPS), AND BUILDING PARTNERSHIPS WITH EMPLOYERS AND SERVICE PROVIDERS IN THE COMMUNITY. THE REQUIRED NUMBER OF PARTICIPATING FAMILIES THAT A GRANTEE MUST MAINTAIN IS OUTLINED IN THE FUNDING NOTICE AND IS BASED ON THE NUMBER OF COORDINATORS FOR WHICH A GRANTEE RECEIVES FUNDING. GENERALLY, THE GRANTEE MUST HIRE/RETAIN ONE FULL-TIME EQUIVALENT (FTE) FSS PROGRAM COORDINATOR FOR EACH FUNDED POSITION. FOR THE FIRST FULL-TIME FSS COORDINATOR POSITION FUNDED BY HUD, PROGRAMS MUST SERVE AT LEAST 25 PARTICIPATING FAMILIES IN FSS DURING THE PERIOD OF PERFORMANCE (THE CALENDAR YEAR FOLLOWING THE APPROPRIATION), AND THIS NUMBER INCREASES BY 50 FAMILIES FOR EACH ADDITIONAL FUNDED COORDINATOR AFTER THE FIRST.; EXPECTED OUTCOMES: GRANTEES WILL HIRE/RETAIN THE NUMBER OF COORDINATORS FOR WHICH THEY ARE FUNDED. THESE COORDINATORS WILL SERVE AT LEAST THE MINIMUM NUMBER OF PARTICIPANTS REQUIRED BY THE GRANT. OVER THE 5-7 YEARS OF THEIR CONTRACT OF PARTICIPATION, PARTICIPANTS WILL BECOME EMPLOYED OR IMPROVE THEIR EMPLOYMENT AND WAGES AND MEET OTHER INDIVIDUAL GOALS.; INTENDED BENEFICIARIES: THE INTENDED BENEFICIARIES OF FSS ARE FAMILIES CURRENTLY RESIDING IN PUBLIC HOUSING (SECTION 9), FAMILIES CURRENTLY PARTICIPATING IN THE HOUSING CHOICE VOUCHER (SECTION 8) PROGRAM, AND THOSE LIVING IN PROJECT-BASED RENTAL ASSISTANCE (PBRA) PROPERTIES. ; SUBRECIPIENT ACTIVITIES: THE RECIPIENT DOES NOT INTEND TO SUBAWARD FUNDS.

RURAL BUSINESS ENTERPRISE GRANTS

TO SUPPORT OPERA-BASED TEACHING AND LEARNING IN PUBLIC ELEMENTARY SCHOOLS IN NEW YORK CITY, NEW JERSEY, AND THE GREATER BOSTON AREA.

PURPOSE: THE FAMILY SELF-SUFFICIENCY PROGRAM (FSS) IS GOVERNED BY SECTION 23 OF THE 1937 HOUSING ACT. THE PURPOSE OF FSS AWARDS IS TO FUND THE SALARIES AND FRINGE FOR FSS COORDINATORS, WHO ARE RESPONSIBLE FOR IMPLEMENTING FSS FOR RESIDENTS OF PUBLIC HOUSING, HOUSING CHOICE VOUCHERS (HCV), AND PROJECT-BASED RENTAL ASSISTANCE (PBRA) PROPERTIES. FSS FUNDS APPROXIMATELY 900 PROGRAMS RUN BY PUBLIC HOUSING AUTHORITIES (PHAS) AND PBRA PROPERTY OWNERS ACROSS THE COUNTRY. RENEWAL APPLICANTS ARE PRIORITIZED BY STATUTE.; ACTIVITIES TO BE PERFORMED: FUNDS UNDER FSS GRANTS ARE USED SOLELY TO FUND SALARY AND FRINGE BENEFITS FOR FSS COORDINATORS. RESPONSIBILITIES OF FSS COORDINATORS UNDER THE AWARD INCLUDE, BUT ARE NOT LIMITED TO, ENROLLING, AND MAINTAINING FAMILIES IN THE PROGRAM, WORKING WITH FAMILIES TO PREPARE INDIVIDUAL TRAINING AND SERVICES PLANS (ITSPS), AND BUILDING PARTNERSHIPS WITH EMPLOYERS AND SERVICE PROVIDERS IN THE COMMUNITY. THE REQUIRED NUMBER OF PARTICIPATING FAMILIES THAT A GRANTEE MUST MAINTAIN IS OUTLINED IN THE FUNDING NOTICE AND IS BASED ON THE NUMBER OF COORDINATORS FOR WHICH A GRANTEE RECEIVES FUNDING. GENERALLY, THE GRANTEE MUST HIRE/RETAIN ONE FULL-TIME EQUIVALENT (FTE) FSS PROGRAM COORDINATOR FOR EACH FUNDED POSITION. FOR THE FIRST FULL-TIME FSS COORDINATOR POSITION FUNDED BY HUD, PROGRAMS MUST SERVE AT LEAST 25 PARTICIPATING FAMILIES IN FSS DURING THE PERIOD OF PERFORMANCE (THE CALENDAR YEAR FOLLOWING THE APPROPRIATION), AND THIS NUMBER INCREASES BY 50 FAMILIES FOR EACH ADDITIONAL FUNDED COORDINATOR AFTER THE FIRST.; EXPECTED OUTCOMES: GRANTEES WILL HIRE/RETAIN THE NUMBER OF COORDINATORS FOR WHICH THEY ARE FUNDED. THESE COORDINATORS WILL SERVE AT LEAST THE MINIMUM NUMBER OF PARTICIPANTS REQUIRED BY THE GRANT. OVER THE 5-7 YEARS OF THEIR CONTRACT OF PARTICIPATION, PARTICIPANTS WILL BECOME EMPLOYED OR IMPROVE THEIR EMPLOYMENT AND WAGES AND MEET OTHER INDIVIDUAL GOALS.; INTENDED BENEFICIARIES: THE INTENDED BENEFICIARIES OF FSS ARE FAMILIES CURRENTLY RESIDING IN PUBLIC HOUSING (SECTION 9), FAMILIES CURRENTLY PARTICIPATING IN THE HOUSING CHOICE VOUCHER (SECTION 8) PROGRAM, AND THOSE LIVING IN PROJECT-BASED RENTAL ASSISTANCE (PBRA) PROPERTIES. ; SUBRECIPIENT ACTIVITIES: THE RECIPIENT DOES NOT INTEND TO SUBAWARD FUNDS.

VALUE- ADDED AGRICULTURAL PRODUCT MARKET DEVELOPMENT GRANTS

PURPOSE: THE FAMILY SELF-SUFFICIENCY PROGRAM (FSS) IS GOVERNED BY SECTION 306 OF THE ECONOMIC GROWTH, REGULATORY RELIEF, AND CONSUMER PROTECTION ACT (PUBLIC LAW NO: 115-174). THE PURPOSE OF FSS AWARDS IS TO FUND THE SALARIES AND FRINGE FOR FSS COORDINATORS, WHO ARE RESPONSIBLE FOR IMPLEMENTING FSS FOR RESIDENTS OF PUBLIC HOUSING, HOUSING CHOICE VOUCHERS (HCV), AND PROJECT-BASED RENTAL ASSISTANCE (PBRA) PROPERTIES. FSS FUNDS MORE THAN 800 PROGRAMS RUN BY PUBLIC HOUSING AUTHORITIES (PHAS) AND PBRA PROPERTY OWNERS ACROSS THE COUNTRY. RENEWAL APPLICANTS ARE PRIORITIZED BY STATUTE, AND NEW AWARD SELECTION IS MADE BY LOTTERY.; ACTIVITIES TO BE PERFORMED: FUNDS UNDER FSS GRANTS ARE USED SOLELY TO FUND SALARY AND FRINGE BENEFITS FOR FSS COORDINATORS. RESPONSIBILITIES OF FSS COORDINATORS UNDER THE AWARD INCLUDE, BUT ARE NOT LIMITED TO, ENROLLING, AND MAINTAINING THE MINIMUM NUMBER OF FAMILIES IN THE PROGRAM, WORKING WITH FAMILIES TO PREPARE INDIVIDUAL TRAINING AND SERVICES PLANS (ITSPS), AND BUILDING PARTNERSHIPS WITH EMPLOYERS AND SERVICE PROVIDERS IN THE COMMUNITY. THE REQUIRED NUMBER OF PARTICIPATING FAMILIES THAT A PROGRAM MUST MAINTAIN IS OUTLINED IN THE NOTICE OF FUNDING OPPORTUNITY (NOFO) AND IS BASED ON THE NUMBER OF COORDINATORS FOR WHICH A GRANTEE RECEIVES FUNDING. GENERALLY, THE GRANTEE MUST HIRE/RETAIN ONE FULL-TIME EQUIVALENT (FTE) FSS PROGRAM COORDINATOR FOR EACH FUNDED POSITION. FOR THE FIRST FULL-TIME FSS COORDINATOR POSITION FUNDED BY HUD, PROGRAMS MUST SERVE AT LEAST 25 PARTICIPATING FAMILIES IN FSS DURING THE PERIOD OF PERFORMANCE (THE CALENDAR YEAR FOLLOWING THE APPROPRIATION), AND THIS NUMBER INCREASES BY 50 FAMILIES FOR EACH ADDITIONAL FUNDED COORDINATOR AFTER THE FIRST.; EXPECTED OUTCOMES: GRANTEES WILL HIRE/RETAIN THE NUMBER OF COORDINATORS FOR WHICH THEY ARE FUNDED. THESE COORDINATORS WILL SERVE AT LEAST THE MINIMUM NUMBER OF PARTICIPANTS REQUIRED BY THE GRANT. OVER THE 5-7 YEARS OF THEIR CONTRACT OF PARTICIPATION, PARTICIPANTS WILL BECOME EMPLOYED OR IMPROVE THEIR EMPLOYMENT AND WAGES AND MEET OTHER INDIVIDUAL GOALS.; INTENDED BENEFICIARIES: THE INTENDED BENEFICIARIES OF FSS ARE FAMILIES CURRENTLY RESIDING IN PUBLIC HOUSING (SECTION 9), FAMILIES CURRENTLY PARTICIPATING IN THE HOUSING CHOICE VOUCHER (SECTION 8) PROGRAM, AND THOSE LIVING IN PROJECT-BASED RENTAL ASSISTANCE (PBRA) PROPERTIES.; SUBRECIPIENT ACTIVITIES: THE RECIPIENT DOES NOT INTEND TO SUBAWARD FUNDS.

THE GUILD WILL PROVIDE TWO INTERNS IN SPRING 2023 AS DESCRIBED IN THIS PROPOSAL SUBJECT TO FUNDING AVAILABILITY AT THE CAROLINA SANDHILLS NWR, THE GUILD WILL PROVIDE INTERNS IN YEAR TWO AND YEAR THREE OF THIS AGREEMENT THE FOREST STEWARDS GUILD WOULD RECRUIT QUALIFIED FORESTRY INTERNS FROM ACCREDITED FORESTRY AND NATURAL RESOURCE PROGRAMS AND PLACE THOSE INTERNS IN ASSIGNMENT S AT REFUGES UNDER YOUR SUPERVISION

FAMILY SELF-SUFFICIENCY PROGRAM

FAMILY SELF-SUFFICIENCY PROGRAM

FAMILY SELF-SUFFICIENCY PROGRAM

FAMILY SELF-SUFFICIENCY PROGRAM

FAMILY SELF-SUFFICIENCY PROGRAM

FAMILY SELF-SUFFICIENCY PROGRAM

FAMILY SELF-SUFFICIENCY PROGRAM

FAMILY SELF-SUFFICIENCY PROGRAM

TO SUPPORT OPERA-BASED TEACHING AND LEARNING IN PUBLIC ELEMENTARY SCHOOLS IN NEW YORK CITY AND NEW JERSEY.

FAMILY SELF-SUFFICIENCY PROGRAM

RBDG RURAL BUSINESS COOP RURAL ENTERPRISE GRANT

TO SUPPORT OPERA-BASED TEACHING AND LEARNING IN PUBLIC ELEMENTARY SCHOOLS IN NEW YORK CITY AND NEW JERSEY.

MULTIFAMILY HOUSING SERVICE COORDINATORS

THIS AGREEMENT WILL ENHANCE MILITARY READINESS BY PREPARING OFF-INSTALLATION AND NEW PERSONNEL TO RESPOND TO THE UNIQUE CHALLENGES ON MILITARY INSTAL

TO SUPPORT OPERA-BASED TEACHING AND LEARNING IN PUBLIC ELEMENTARY SCHOOLS IN NEW YORK CITY AND NEW JERSEY.

TO SUPPORT THE NATIONAL CONFERENCE FOR COMMUNITY ARTS EDUCATION IN CHICAGO AND A LEADERSHIP DEVELOPMENT INSTITUTE IN PHILADELPHIA.

RURAL BUSINESS ENTERPRISE GRANTS

TO SUPPORT THE DISSEMINATION OF OPERA INSTITUTE PRACTICE.

OREGON ARTISAN, FARMSTEAD, AND SPECIALTY CHEESE: DIRECT MARKETING AND INFRASTRUCTURE INITIATIVE

RBDG RURAL BUSINESS COOP RURAL ENTERPRISE GRANT

COMMUNITY FACILITY GRANTS

RURAL BUSINESS ENTERPRISE GRANTS

PURPOSE: TO SUPPORT AN OPERA EDUCATION PROGRAM FOR STUDENTS AND PROFESSIONAL DEVELOPMENT FOR TEACHERS.

TO SUPPORT PERSONNEL COSTS IN RESPONSE TO THE COVID-19 PANDEMIC.

TO SUPPORT PERSONNEL COSTS IN RESPONSE TO THE COVID-19 PANDEMIC.

TO SUPPORT PERSONNEL COSTS IN RESPONSE TO THE COVID-19 PANDEMIC.

TO SUPPORT PERSONNEL COSTS IN RESPONSE TO THE COVID-19 PANDEMIC.

TO SUPPORT PERSONNEL COSTS IN RESPONSE TO THE COVID-19 PANDEMIC.

TO SUPPORT CREATIVE YOUTH DEVELOPMENT PEER LEARNING NETWORKS A COLLECTIVE IMPACT INITIATIVE

TO SUPPORT YEAR-ROUND OPERA-BASED TEACHING AND LEARNING IN TITLE I PUBLIC ELEMENTARY SCHOOLS IN NEW YORK CITY AND NEW JERSEY.

TO SUPPORT THE CONTINUED IMPLEMENTATION OF THE NATIONAL ACTION BLUEPRINT FOR CREATIVE YOUTH DEVELOPMENT A COLLECTIVE IMPACT PROJECT.

TO SUPPORT OPERA-BASED TEACHING AND LEARNING IN PUBLIC ELEMENTARY SCHOOLS IN NEW YORK CITY AND NEW JERSEY.

TO SUPPORT OPERA-BASED TEACHING AND LEARNING IN PUBLIC ELEMENTARY SCHOOLS IN NEW YORK CITY AND NEW JERSEY.

TO SUPPORT OPERA-BASED TEACHING AND LEARNING IN PUBLIC ELEMENTARY SCHOOLS IN NEW YORK CITY, NEW JERSEY, AND THE GREATER BOSTON AREA.

TO SUPPORT THE NATIONAL CONFERENCE FOR COMMUNITY ARTS EDUCATION IN DALLAS, THE COMMUNITY ARTS EDUCATION LEADERSHIP INSTITUTE AND REGIONAL TRAINING AN

TO SUPPORT THE NATIONAL CONFERENCE FOR COMMUNITY ARTS EDUCATION IN BOSTON, A LEADERSHIP DEVELOPMENT INSTITUTE IN PHILADELPHIA, AND REGIONAL PROFESSIO

THIS PROJECT WILL CUT DOWN AND REMOVE HAZARDOUS TREES ON AND AROUND THE NATIVE AMERICAN BURIAL MOUNDS IN THE NORTH UNIT AT EFFIGY MOUNDS NATIONAL MONUMENT. THE REMOVAL WILL BE DONE IN ACCORDANCE WITH THE APPROVED CULTURAL LANDSCAPE REPORT AND TO PROTECT THE BURIAL MOUNDS.

RBDG RURAL BUSINESS COOP RURAL ENTERPRISE GRANT

COMMUNITY FACILITY 09/10 STIMULUS GRANTS

TO SUPPORT "CLAY CONNECTIONS: BRIDGING HIGH SCHOOL AND HIGHER EDUCATION THROUGH REGIONAL AND GLOBAL ART" IN CONSORTIUM WITH WEST VIRGINIA UNIVERSITY.

TO SUPPORT YEAR-ROUND OPERA-BASED TEACHING AND LEARNING IN TITLE I PUBLIC ELEMENTARY SCHOOLS IN NEW YORK CITY AND NEW JERSEY.

TO SUPPORT PROFESSIONAL DEVELOPMENT PROGRAMS FOR LEADERS IN THE ARTS EDUCATION FIELD.

FUELS TREATMENT EVALUATION AT ROCKY MOUNTAIN NATIONAL PARK

TO SUPPORT THE LOUISIANA ARTWORKS RESIDENCY PROGRAM.

TO SUPPORT PROFESSIONAL DEVELOPMENT PROGRAMS FOR LEADERS IN THE ARTS EDUCATION FIELD.

PURPOSE: TO SUPPORT A NATIONAL TOURING INITIATIVE CELEBRATING FREEDOM250 AND ACCOMPANYING EDUCATIONAL ENGAGEMENT ACTIVITIES.

USING TIMBER GRADING TO CAPITALIZE ON HIDDEN CAPACITY IN EXISTING TIMBER STRUCTURES

PURPOSE: TO SUPPORT THE VETERANS WRITING PROJECT AND VETERANS FELLOWSHIP PROGRAMS.

RBDG RURAL BUSINESS COOP OPPORTUNITY GRANT

TO SUPPORT PROFESSIONAL DEVELOPMENT PROGRAMS FOR LEADERS IN THE ARTS EDUCATION FIELD.

TO PRESENT ENGLISH LANGUAGE PROGRAMMING, BUILT AROUND AND INCLUDING A VERSION OF SEUSSICAL TO BE PRESENTED AT PANAMA BOOK FAIR.

TO SUPPORT A YOUTH-FOCUSED CREATIVE PLACEMAKING TRAINING PROGRAM IN PUEBLO COUNTY COLORADO.

TO SUPPORT THE CAREER DEVELOPMENT OF EMERGING CLASSICAL MUSICIANS THROUGH NATIONAL CONCERT ENGAGEMENTS.

TO SUPPORT THE NEW RHYTHMS MINI-CONCERT SERIES AND RELATED EDUCATIONAL ACTIVITIES.

PURPOSE: TO SUPPORT STAFF SALARY COSTS AND AUTHOR FEES TOWARD EDUCATIONAL PROGRAMMING AND ONLINE RESOURCES FOR WRITERS.

COMMUNITY FACILITY EZ/EC GRANTS

PURPOSE: TO SUPPORT ARTS EDUCATION PROGRAMMING FOR YOUTH.

PURPOSE: TO SUPPORT THE PROFESSIONAL DEVELOPMENT OF EMERGING MUSICIANS THROUGH TOURING AND COMMUNITY ENGAGEMENT ACTIVITIES ACROSS THE UNITED STATES.

PURPOSE: TO SUPPORT FREE PROFESSIONAL DEVELOPMENT WEBINARS FOR WRITERS.

TO SUPPORT EDUCATIONAL PROGRAMS AND CAREER DEVELOPMENT FOR ORGANISTS AND CHORAL CONDUCTORS.

TO SUPPORT EDUCATIONAL PROGRAMS AND CAREER DEVELOPMENT FOR ORGANISTS CHORAL CONDUCTORS AND COMPOSERS.

TO SUPPORT EDUCATIONAL PROGRAMS AND CAREER DEVELOPMENT FOR ORGANISTS, CHORAL CONDUCTORS, AND COMPOSERS.

TO SUPPORT THE HELEN DEUTSCH VETERANS' WRITING WORKSHOPS.

CREATING A FOOD HUB FOR THE SAN JUAN

TO SUPPORT A SERIES OF PERFORMING AND VISUAL ARTS EVENTS.

TO SUPPORT TOURING ENGAGEMENTS OF ROSTER ARTISTS AND THE COMMISSIONING OF NEW WORKS.

TO SUPPORT TOURING ENGAGEMENTS OF ROSTER ARTISTS AND THE COMMISSIONING OF NEW WORKS.

TO SUPPORT PERFORMANCES OF MONTEVERDI'S "VESPERS OF 1610 " INCLUDING ASSOCIATED EDUCATIONAL ACTIVITIES IN CONSORTIUM WITH THE SEATTLE BAROQUE ORCHEST

TO SUPPORT THE PRESERVATION OF ARCHIVAL RECORDINGS OF PAST MCG JAZZ SUBSCRIPTION SERIES CONCERTS.

CREATING A FOOD HUB FOR THE SAN JUAN

FY 2023 BRIDGE ACCESS PROGRAM

TOURISM DEVELOPMENT

TO SUPPORT TOURING ENGAGEMENTS OF ROSTER ARTISTS AND THE COMMISSIONING OF A NEW WORK. THE COMMISSION SCORE IS REQUIRED AS A FINAL PRODUCT.

TO SUPPORT TOURING ENGAGEMENTS OF ROSTER ARTISTS AND THE COMMISSIONING OF NEW WORKS.

PURPOSE: TO SUPPORT THE OPERA LEARNING INSTITUTE, A WEEK-LONG PROFESSIONAL DEVELOPMENT INTENSIVE FOR TEACHERS.

PURPOSE: TO SUPPORT AN ARTIST RESIDENCY PROGRAM.

PURPOSE: TO SUPPORT AN ARTIST-IN-RESIDENCE PROGRAM FEATURING ACTRESS SINGER DIRECTOR AND PRODUCER TAMARA TUNIE.

PURPOSE: TO SUPPORT THE VETERANS WRITING PROJECT.

PURPOSE: TO SUPPORT THE VETERANS WRITING PROJECT.

PURPOSE: TO SUPPORT TOURING ENGAGEMENTS FOR ARTISTS MANAGED BY CONCERT ARTISTS GUILD.

PURPOSE: TO SUPPORT IN-SCHOOL MULTIDISCIPLINARY ARTS WORKSHOPS.

PURPOSE: TO SUPPORT ARTS PROGRAMMING IN THE PUEBLO CREATIVE CORRIDOR IN PUEBLO COUNTY COLORADO.

TO SUPPORT GENERAL OPERATING COSTS IN RESPONSE TO THE COVID-19 PANDEMIC.

TO SUPPORT EDUCATIONAL PROGRAMS AND CAREER DEVELOPMENT FOR ORGANISTS AND CHORAL CONDUCTORS.

TO SUPPORT THE HELEN DEUTSCH WRITING WORKSHOPS.

TO SUPPORT A MULTIDISCIPLINARY SERIES IN JOSHUA TREE NATIONAL PARK.

TO SUPPORT A NATIONAL CONFERENCE AND LEADERSHIP INSTITUTE.

TO SUPPORT COMMISSIONS AND EDUCATIONAL OUTREACH PROGRAMS. COPIES OF THE COMMISSIONED WORKS ARE REQUIRED PRODUCTS.

TO SUPPORT CAPACITY BUILDING ACTIVITIES AND SERVICES TO THE FIELD.

TO SUPPORT THE HELEN DEUTSCH VETERANS' WRITING WORKSHOPS IN SAN ANTONIO.

TO SUPPORT THE MCG JAZZ 25TH ANNIVERSARY ONCE IN A LIFETIME CONCERTS.

TO SUPPORT THE 50TH BIENNIAL NATIONAL CONVENTION.

TO SUPPORT CAREER DEVELOPMENT OF EMERGING CLASSICAL MUSICIANS THROUGH NATIONAL CONCERT ENGAGEMENTS.

COPL CESU: SUPERIOR UPLANDS POTENTIAL NATIONAL NATURAL LANDMARK EVALUATION

REDUCE EROSION PRCESSES ALONG TWO ARROYO SYSTEMS