Gamm Incorporated

TO SUPPORT THE UGANDA MINISTRY OF HEALTH TO EXECUTE ITS CORE MANDATE FOR A HARMONIZED AND SUSTAINABLE HEALTH SECTOR RESPONSE TO HIV/AIDS AND OTHER INFECTIOUS DISEASES IN THE REPUBLIC OF UGANDA.

STRENGTHENING HIV/AIDS PREV, CARE, TREATMENT, SUPPORT & STRATEGIC INFORMATION IN

STRENGTHENING UGANDA MINISTRY OF HEALTH TECHNICAL CAPACITY TO STANDARDIZE, HARMONIZE, AND PROVIDE OVERSIGHT FOR A SUSTAINABLE PUBLIC HEALTH RESPONSE TO HIV/AIDS, TB, AND RELATED HEALTH EFFORTS.

SUPPORT UGANDA MINISTRY OF HEALTH'S CAPACITY TO ADDRESS HIV RESPONSE THROUGH STRE

MOLECULAR BREAST IMAGING TO GUIDE EARLY-STAGE PATIENT CARE

VENOUS THROMBOEMBOLISM RISK MARKER ASSAY

AUTOMATED 3D DATA FUSION SURVEYS ON SPOT ROBOT FOR NEXT GENERATION PLANT MONITORING

DUAL HEADED CZT BREAST IMAGER

REAP RENEWABLE ENERGY SYSTEM (RES) GRANT UNRESTRICTED AMOUNT

SBIR PHASE II: BISTABLE ELECTROFLUIDIC DEVICE FOR HIGH-BRIGHTNESS ELECTRONIC PAPER

WASTE DISPOSAL GRANTS - REGULAR

ACT AGAINST AIDS LEADERSHIP INITIATIVE

SBIR PHASE II: ACTIVE VISIBLE AND INFRARED MANAGEMENT FOR MORE ENERGY EFFICIENT BUILDINGS

MULTIFAMILY HOUSING SERVICE COORDINATORS

DUAL MODALITY SPECT-MRI FOR IN-VIVO SMALL ANIMAL IMAGING

DEVELOPMENT OF ANTISENSE OLIGONUCLEOTIDES TO REGULATE GAMMA' FIBRINOGEN LEVELS - PROJECT SUMMARY/ABSTRACT G' (PRONOUNCED "GAMMA PRIME") FIBRINOGEN (GPF) IS AN ALTERNATIVE SPLICE ISOFORM OF THE BLOOD COAGULATION FACTOR FIBRINOGEN. THIS FIBRINOGEN VARIANT CONTAINS A HIGH AFFINITY BINDING SITE FOR THE COAGULATION FACTOR THROMBIN THAT LOCALIZES THROMBIN ON THE GROWING BLOOD CLOT. THROMBIN BINDS TO GPF VIA THROMBIN'S HEPARIN BINDING SITE. THIS ALLOWS THROMBIN TO CONTINUE FORMING THE BLOOD CLOT EVEN IN THE PRESENCE OF ITS INHIBITOR PROTEIN, ANTITHROMBIN III, WITH THE COFACTOR HEPARIN. IN ADDITION, GPF FORMS CLOTS THAT ARE RESISTANT TO BREAKDOWN BY FIBRINOLYTIC ENZYMES, SUCH THAT THE CLOTS PERSIST IN THE BLOOD VESSEL. BECAUSE OF THESE ACTIVITIES, GPF IS A RISK FACTOR FOR CARDIOVASCULAR DISEASE, INCLUDING HEART ATTACKS AND STROKE. WE HAVE RECENTLY FOUND THAT COVID-19 PATIENTS CAN DEVELOP EXTRAORDINARILY HIGH LEVELS OF GPF, WHICH LIKELY CONTRIBUTES TO THE THROMBOTIC EVENTS THAT ARE SEEN IN COVID-19 PATIENTS. IN SUPPORT OF THIS HYPOTHESIS, CRITICALLY ILL COVID-19 PATIENTS ARE HEPARIN RESISTANT AND DO NOT BENEFIT FROM HEPARIN TREATMENT. HIGH GPF LEVELS LIKE THESE SEQUESTER THROMBIN IN AN ACTIVE FORM THAT CANNOT BE INHIBITED BY ANTITHROMBIN III/HEPARIN. IF THE GPF LEVELS COULD BE LOWERED, MANY OF THESE THROMBOTIC EVENTS COULD LIKELY BE PREVENTED. THE SPECIFIC AIMS OF THIS APPLICATION ARE THEREFORE TO: 1) DEVELOP ANTISENSE MORPHOLINO OLIGOS THAT BIND THEIR TARGET G GENE SEQUENCES AND ARE TAKEN UP BY THE HEPG2 HUMAN LIVER CELL LINE. THIS WILL BE ACCOMPLISHED USING ELECTROPHORETIC MOBILITY SHIFT ASSAYS TO ENSURE THAT THE ANTISENSE OLIGOS BIND TO THEIR TARGET SEQUENCES. CELLULAR UPTAKE INTO LIVER CELLS WILL BE ASSAYED USING FLUORESCEIN-LABELED OLIGOS IN A WELL- ESTABLISHED TISSUE CULTURE MODEL OF FIBRINOGEN SYNTHESIS, THE HUMAN HEPG2 LIVER CELL LINE. THIS CELL LINE EXPRESSES BOTH THE GA AND G' ISOFORMS OF THE HUMAN G CHAIN. 2) OPTIMIZE THE MORPHOLINO OLIGOMERS FOR EFFECTIVENESS IN VIVO USING HEPG2 CELLS. THIS WILL BE ACCOMPLISHED BY ASSAYING THE LEAD COMPOUNDS DEVELOPED IN AIM 1 IN HEPG2 CELLS. THE ABSOLUTE LEVELS AND THE RELATIVE EXPRESSION RATIOS OF THE GA AND G' MRNAS WILL BE QUANTITATED USING RT-PCR. TOTAL FIBRINOGEN AND GPF WILL BE MEASURED BY ELISA. MILESTONES – THE CRITERIA FOR PROGRESS TO ANIMAL STUDIES IN PRIMATES IN PHASE II ARE: 1. DEVELOPMENT OF ANTISENSE MORPHOLINO OLIGOMERS THAT BIND THEIR TARGET G GENE SEQUENCES. 2. DEMONSTRATION THAT THE ANTISENSE MORPHOLINO OLIGOMERS MODULATE GPF LEVELS IN HEPG2 LIVER CELLS.

STTR PHASE I: RAPID HEMOSTATIC DRESSING FOR HEMORRHAGE CONTROL

AUTONOMOUS TECHNOLOGIES FOR OPTIMIZED MAPPING AND INTELLIGENT CHARACTERIZATION OF RADIOLOGICAL SOURCES

ADVANCED PORTABLE LOW TEMPERATURE PLASMA DEVICE FOR CHRONIC WOUND DISINFECTION AND HEALING

SBIR PHASE I: BISTABLE ELECTROFLUIDIC DEVICE FOR HIGH-BRIGHTNESS ELECTRONIC PAPER

SBIR PHASE I: ACTIVE VISIBLE AND INFRARED MANAGEMENT FOR MORE ENERGY EFFICIENT BUILDINGS

PURPOSE: THE PURPOSE OF THIS PROJECT IS RESEARCH WHETHER AND TO WHAT EXTENT VARIOUS AUTHORIZING AGENCIES OF THE UNITED STATES OF AMERICA INCLUDES CYBERSECURITY REQUIREMENTS DE JURE OR DE FACTO AS PART OF THEIR AUTHORIZING ACTIVITIES OR THEIR MANDATORY CONTINUING SUPERVISION FOR AUTHORIZED COMMERCIAL, NON-GOVERNMENTAL ACTIVITIES SUCH AS COMMUNICATIONS AND REMOTE SENSING SATELLITES, SPACE TRAVEL/TOURISM SUPPORT, ON-ORBIT MANUFACTURING, AND SPACE MISSION SUPPORT. ACTIVITIES TO BE PERFORMED: IN COLLABORATION WITH THE U.S. DEPARTMENT OF COMMERCE, OFFICE OF SPACE COMMERCE, THE PRINCIPALINVESTIGATOR WILL GENERATE A REPORT TO INCLUDE A SURVEY, DISCUSSION, AND RECOMMENDATIONS CONCERNINGIMPORTANT ASPECTS OF CYBERSECURITY PRACTICES IN THE FRAMEWORK OF STANDARDS AND SPACE MISSIONAUTHORIZATION FOR COMMERCIAL SPACE MISSIONS TO MEET THE OBJECTIVE TO ADVANCE THE STATE-OF-THE-ART NORMS FOR CONDUCT IN CYBERSPACE. THE OBJECTIVE WILL BE MET THROUGH A SYNTHESIS AND REVIEW OF GOVERNMENT INFORMATION SOURCES, INCLUDING EVENTS THE U.S. DEPARTMENT OF COMMERCE HAS LED OR PARTICIPATED IN COLLABORATIVELY, AND SUPPLEMENTED BY INTERVIEWS WITH PUBLIC AND PRIVATE SECTOR ACTORS. IN PHASE I THE PI WILL COLLABORATE WITH THE DEPARTMENT OF COMMERCE TO ORGANIZE, LEAD, AND RESEARCHRELEVANT STAKEHOLDER MATERIAL TO ADVANCE THE STATE-OF-THE-ART NORMS FOR CONDUCT IN CYBERSPACE WITHRESPECT TO SPACE MISSION AUTHORIZATIONS. IN PHASE II THE PI WILL PREPARE A REPORT FOR PUBLICATION IN THEPEER REVIEWED LITERATURE TO INCLUDE A SURVEY, DISCUSSION, AND RECOMMENDATIONS AS OUTLINED IN THENARRATIVE.EXPECTED OUTCOMES: THE PUBLICATION WILL ENABLE A REFERENCE DOCUMENT TO PROVIDE AN ASSESSMENT OF THE CURRENT LANDSCAPE FOR SPACE CYBERSECURITY APPROACHES WITH RESPECT TO SPACE MISSION AUTHORIZATION.INTENDED BENEFICIARIES: THE INFORMATION REPORTED FROM THIS EFFORT CAN BE USED BY U.S. AND INTERNATIONAL AGENCIES WORKING ON THE DEVELOPMENTS AND APPLICATION OF SPACE CYBERSECURITY FRAMEWORKS, GUIDELINES, AND STANDARDS. THE PUBLICATION WILL BE AVAILABLE ONLINE AND FREE OF CHARGE TO THE PUBLIC THAT CAN BE USED BY ALL ENTITIES WORKING TO UNDERSTAND CYBERSECURITY NEEDS FOR SPACE SYSTEMS, INCLUDING STUDENTS.SUBRECIPIENT ACTIVITIES: THE RECIPIENT DOES NOT INTEND TO SUBAWARD FUNDS.

COMMUNITY FACILITY GRANTS - COMMUNITY FACILITY

TO SUPPORT COMMUNITY PLANNING AND VISIONING OF A CULTURAL ANCHOR FOR PAWTUCKET.

SEC. 9007 REAP-ENERGY EFFICIENCY IMPROVEMENTS GRANTS (MAN)

SEC. 9007 REAP-ENERGY EFFICIENCY IMPROVEMENTS GRANTS (MAN)

MAN. VALUE-ADDED AG PMDG-MID TIER CHAINS

SRSA APPLICATION

APPLICATION FOR SMALL RURAL SCHOOL ACHIEVEMENT PROGRAM

OFFICE OF ELEMENTARY EDUCATION: SMALL, RURAL SCHOOL ACHIEVEMENT PROGRAM

APPLICATION FOR SMALL, RURAL SCHOOL ACHIEVEMENT PROGRAM

APPLICATION FOR SMALL, RURAL SCHOOL ACHIEVEMENT PROGRAM

APPLICATION FOR SMALL RURAL SCHOOL ACHIEVEMENT PROGRAM

APPLICATION FOR SMALL RURAL SCHOOL ACHIEVEMENT PROGRAM

SMALL, RURAL SCHOOL ACHIEVEMENT PROGRAM

COMMUNITY FACILITY GRANTS

APPLICATION FOR SMALL, RURAL SCHOOL ACHIEVEMENT PROGRAM

APPLICATION FOR SMALL, RURAL SCHOOL ACHIEVEMENT PROGRAM

SMALL, RURAL SCHOOL ACHIEVEMENT PROGRAM

APPLICATION FOR SMALL, RURAL SCHOOL ACHIEVEMENT PROGRAM

SMALL, RURAL SCHOOL ACHIEVEMENT PROGRAM

SMALL, RURAL SCHOOL ACHIEVEMENT PROGRAM

SMALL, RURAL SCHOOL ACHIEVEMENT PROGRAM

SMALL, RURAL SCHOOL ACHIEVEMENT PROGRAM

TO SUPPORT THE PRODUCTION OF AN OCTOROON BY BRANDEN JACOBS-JENKINS.

SMALL, RURAL SCHOOL ACHIEVEMENT PROGRAM

RURAL EDUCATION ACHIEVEMENT PROGRAM

GRANT ISSUED TO COVER FEES FOR 3 SPEAKERS TO GIVE PRESENTATIONS AT GAMMEL HOLTEGAARD MUSEUM IN CONNECTIN WITH AN EXHIBITION OF THE WORKS OF AMERICAN

ENVIRONMENTAL QUALITY INCENTIVE PROGRAM

ASSISTANCE TO FIREFIGHTERS GRANT

SUPPORT UGANDA MINISTRY OF HEALTH'S CAPACITY TO ADDRESS HIV RESPONSE THROUGH STRE