U School

IMPACT AID PROGRAM, TITLE VII, SECTION 7003

IMPACT AID PROGRAM, TITLE VII, SECTION 7003

IMPACT AID PROGRAM, TITLE VII, SECTION 7003

IMPACT AID PROGRAM, TITLE VII, SECTION 7003

IMPACT AID PROGRAM, TITLE VII, SECTION 7003

IMPACT AID PROGRAM, TITLE VII, SECTION 7003

UNKNOWN TITLE

UNKNOWN TITLE

VALUE-BASED MEDICAL STUDENT EDUCATION TRAINING PROGRAM - ROWAN UNIVERSITY SCHOOL OF OSTEOPATHIC MEDICINE, THE LARGEST MEDICAL SCHOOL IN NEW JERSEY, IN PARTNERSHIP WITH AN FQHC AND FOUR LARGE HEALTH SYSTEMS ACROSS THE STATE, WILL ENROLL 69 MEDICAL STUDENTS IN A PATHWAYS TO PRIMARY CARE PROGRAM THAT WILL INSPIRE PARTICIPANTS TO CHOOSE RESIDENCIES IN PRIMARY CARE AND PRACTICE IN RURAL AND UNDERSERVED AREAS OF NEW JERSEY. NEW JERSEY IS FACING A SIGNIFICANT SHORTAGE OF PRIMARY CARE PHYSICIANS WITH ONLY A 66 PERCENT ABILITY TO MEET PROJECTED NEEDS BY 2036. THIS SHORTAGE IS PARTICULARLY SIGNIFICANT IN FAMILY MEDICINE, WHICH IS PROJECTED TO MEET ONLY 48 PERCENT OF THE NEED. COUPLED WITH THE PHYSICIAN SHORTAGE IS THE MALDISTRIBUTION OF PHYSICIANS ACROSS THE STATE, WITH THE SOUTHERN COUNTIES, INCLUDING SALEM AND CUMBERLAND COUNTIES, FACING EXTREME LEVELS OF PHYSICIAN SHORTAGES. IN SALEM COUNTY, THERE ARE JUST 16 PRIMARY CARE PHYSICIANS FOR A POPULATION OF 65,338 RESIDENTS, REPRESENTING 0.22 PERCENT OF THE PRIMARY CARE PHYSICIAN WORKFORCE OF NEW JERSEY. THE PHYSICIAN SHORTAGE HAS TRANSLATED TO THE WORST HEALTH OUTCOMES AND LIFE EXPECTANCIES IN SALEM AND NEARBY CUMBERLAND COUNTIES IN NEW JERSEY. TO ADDRESS PHYSICIAN SHORTAGES, ROWANSOM EXPANDED ITS MEDICAL SCHOOL TO INCLUDE A NEW CAMPUS THAT OPENED IN JULY 2022. ROWANSOM CURRENTLY ENROLLS 288 STUDENTS EACH YEAR MAKING IT THE LARGEST MEDICAL SCHOOL IN NEW JERSEY. IN 2024, ROWAN MEDICINE OPENED A NEW CAMPUS IN CUMBERLAND COUNTY THAT WILL PROVIDE INCREASED TRAINING FOR MEDICAL STUDENTS IN THE SALEM/CUMBERLAND REGIONS. CURRENTLY, OVER 46.5 PERCENT OF ROWANSOM STUDENTS MATCH TO A PRIMARY CARE RESIDENCY, AND 50 PERCENT OF OUR ALUMNI PRACTICE IN NEW JERSEY AFTER GRADUATION. ROWANSOM PROPOSES A PATHWAYS TO CAREERS IN PRIMARY CARE PROGRAM THAT WILL INTERFACE WITH THESE DEVELOPMENTS TO BUILD THE PHYSICIAN WORKFORCE ACROSS THAT STATE WITH A PARTICULAR EMPHASIS ON THE SALEM AND CUMBERLAND COUNTY REGIONS, WHICH ARE MOST IN NEED. KEY COMPONENTS OF THE PROGRAM INCLUDE DID ACTIC LECTURES IN PRIMARY CARE FOCUSED ON EMERGING TOPICS THAT AFFECT THE HEALTH OF RESIDENTS IN UNDERSERVED AREAS, A PRIMARY CARE SCHOLARS PROGRAM THAT WILL OFFER HALF-TUITION SCHOLARSHIPS TO 54 PARTICIPANTS OVER THREE YEARS, AND CLINICAL IMMERSION EXPERIENCES IN PEDIATRICS, INTERNAL MEDICINE, AND FAMILY MEDICINE IN RURAL AND MEDICAL UNDERSERVED AREAS OF NEW JERSEY THROUGH PARTNERSHIPS WITH SOUTHERN JERSEY FAMILY MEDICAL CENTERS, VIRTUA OUR LADY OF LOURDES HOSPITAL, CAREPOINT, INSPIRA HEALTH, CAPITAL HEALTH, ROWAN FAMILY MEDICINE AND THE REGIONAL INTEGRATED SPECIAL NEEDS CENTER. OTHER INNOVATIVE COMPONENTS INCLUDE MOBILE OSTEOPATHIC MANIPULATIVE MEDICINE CLINICS IN MEDICALLY UNDERSERVED AREAS, A CAREERS IN PRIMARY CARE SEMINAR SERIES, AND ACADEMIC SUPPORT SERVICES THAT INCLUDE TUTORING, WELLNESS, AND EXAMINATION PREPARATION SERVICES TO ENHANCE RETENTION. STUDENTS WILL ALSO PARTICIPATE IN A PRIMARY CARE CAREERS RESEARCH EXPERIENCES TO ACCELERATE TRAINING AND EDUCATION CALLED CREATE, PROGRAM IN THE SUMMER MONTHS, DURING WHICH THEY WILL WORK WITH PRIMARY CARE PHYSICIANS TO DESIGN PROJECTS TO ENHANCE PRIMARY CARE PRACTICE. ACCELERATED PROGRAMS BEING DEVELOPED INCLUDE A 3 PLUS 4 PROGRAM WITH A COMMUNITY COLLEGE AND A 3 PLUS 3 PLUS 3 PROGRAM THAT WILL ALLOW INDIVIDUALS TO COMPLETE UNDERGRADUATE, MEDICAL SCHOOL, AND A PRIMARY CARE RESIDENCY IN JUST 9 YEARS AFTER HIGH SCHOOL. A POSTBACCALAUREATE BRIDGE TO PRIMARY CARE PROGRAM WILL PROVIDE ACADEMIC SUPPORT TO STUDENTS SO THEY CAN SUCCESSFULLY ENROLL IN MEDICAL SCHOOL AND ENROLL IN THE PATHWAYS PROGRAM. THE PROJECT WILL BE LED BY THE SENIOR ASSOCIATE DEAN FOR FACULTY AFFAIRS AT ROWANSOM. A PROCESS AND OUTCOME EVALUATION OF THE PROJECT IS PLANNED THROUGH THE OFFICE OF THE DEAN.

HEALTH CAREERS OPPORTUNITY PROGRAM

IMPACT AID PROGRAM, TITLE VIII, SECTION 8003

IMPACT AID PROGRAM, TITLE VII, SECTION 7003

IMPACT AID PROGRAM, TITLE VIII, SECTION 8003

IMPACT AID PROGRAM, TITLE VIII, SECTION 8003

GERIATRICS WORKFORCE ENHANCEMENT PROGRAM

IMPACT AID PROGRAM, TITLE VIII, SECTION 8003

IMPACT AID PROGRAM, TITLE VIII, SECTION 8003

IMPACT AID PROGRAM TITLE VIII SECTION 8003

SCHOOL CLIMATE TRANSFORMATION GRANTS TO LEAS

IMPACT AID PROGRAM, TITLE VII, SECTION 7003

IMPACT AID PROGRAM TITLE VIII SECTION 8003

GERIATRICS WORKFORCE ENHANCEMENT PROGRAM

MODEL STATE-SUPPORTED AREA HEALTH EDUCATION CENTERS

GERIATRICS WORKFORCE ENHANCEMENT PROGRAM

EFFECTS OF HURRICANE SANDY ON FUNCTIONAL LIMITATION TRAJECTORIES OF OLDER PEOPLE

COMMUNITY HEALTH WORKER TRAINING PROGRAM - ROWAN SCHOOL OF OSTEOPATHIC MEDICINE PROPOSES A DUAL COMMUNITY HEALTH WORKER (CHW)-CERTIFIED PEER RECOVERY SPECIALIST (CPRS) APPRENTICESHIP PROGRAM TO INTEGRATE HEALTH INTO RECOVERY SUPPORT EDUCATION IN NEW JERSEY. THE PROJECT WILL ENGAGE 240 INDIVIDUALS IN THE NEW DUAL CERTIFICATION PROGRAM, 135 WILL RECEIVE NEW CERTIFICATIONS, 45 ENROLLED IN A REGISTERED APPRENTICESHIP PROGRAM, AND 60 CURRENT CHWS OR CPRSS WILL RECEIVE ENHANCED SKILLS TRAINING. ROWAN UNIVERSITY SCHOOL OF OSTEOPATHIC MEDICINE PROPOSES A PARTNERSHIP WITH THE NEW JERSEY DEPARTMENT OF HEALTH COLETTE LAMOTHE-GALETTE COMMUNITY HEALTH WORKER INSTITUTE AND THE NEW JERSEY DIVISION OF MENTAL HEALTH AND ADDICTION SERVICES TO DEVELOP A DUAL COMMUNITY HEALTH WORKER (CHW) AND CERTIFIED PEER RECOVERY SPECIALIST (CPRS) APPRENTICESHIP PROGRAM. THE PURPOSE OF THE PROGRAM IS TO EXPAND THE PUBLIC HEALTH WORKFORCE THAT IS ABLE TO PROVIDE BOTH RECOVERY SUPPORT AND PROVIDE ESSENTIAL PUBLIC HEALTH SERVICES TO ADDRESS CHRONIC DISEASES IN UNDERSERVED COMMUNITIES IN NEW JERSEY THAT HAVE BEEN DEVASTATED BY BOTH COVID-19 AND THE OPIOID OVERDOSE EPIDEMIC. THROUGH THE PROGRAM, ROWANSOM WILL TRAIN 180 INDIVIDUALS WHO ARE DUALLY CERTIFIED AS BOTH COMMUNITY HEALTH WORKERS AND CERTIFIED PEER RECOVERY SPECIALISTS AND EXTEND THE KNOWLEDGE AND SKILLS OF 60 INDIVIDUALS WITH ONLY A CHW OR CPRS CERTIFICATION. THE GEOGRAPHIC CATCHMENT AREA FOR THE PROJECT WILL BE SIX COUNTIES IN SOUTHERN NEW JERSEY WITH THE WORST HEALTH OUTCOMES AND THE HIGHEST OVERDOSE RATES IN THE STATE. RESEARCH SHOWS THAT INDIVIDUALS WITH SUBSTANCE USE DISORDERS (SUDS) ARE AT HIGHER RISK FOR ONE OR MORE CHRONIC HEALTH CONDITIONS AND THAT HOSPITALIZATION IS MORE PREVALENT IN PATIENTS WITH SUDS AND A CHRONIC HEALTH CONDITION COMPARED TO THOSE WITHOUT AN SUD. RECENT STUDIES SHOW THAT OVERDOSE DEATHS CONTINUE TO WORSEN FOR BLACK OR AFRICAN AMERICAN PEOPLE, BUT RATES FOR OTHER RACE AND ETHNICITY GROUPS HELD STEADY OR DECREASED. DURING THE PANDEMIC, O VERDOSE DEATH RATES PER 100,000 BLACK OR AFRICAN AMERICAN INDIVIDUALS INCREASED FROM 24.7 IN 2019 TO 36.8 IN 2020, WHICH WAS 16.3% HIGHER THAN THAT FOR WHITE INDIVIDUALS (31.6) IN 2020. OTHER STUDIES SHOWED THAT PEOPLE WITH SUDS HAD AN INCREASED RISK FOR COVID-19 AND EXPERIENCED WORSE OUTCOMES. HOWEVER, DESPITE HIGH RATES OF CHRONIC HEALTH CONDITIONS IN INDIVIDUALS WITH SUDS AND INCREASED RISK FOR RELAPSE TO OPIATES AND OTHER DRUGS WHEN PATIENTS DEVELOP A CHRONIC HEALTH CONDITION, THE COMMUNITY HEALTH WORKER PROFESSION HAS REMAINED SEGREGATED FROM THE SUBSTANCE USE TREATMENT FIELD. THIS SEGREGATION MAY BE CONTRIBUTING TO HEALTH INEQUITIES IN THE SUBSTANCE USE POPULATION AND EXACERBATING THE OPIATE OVERDOSE EPIDEMIC. THIS PROJECT WILL EXPLORE THE CREATION OF A NEW COMMUNITY HEALTH WORKER-CERTIFIED PEER RECOVERY SPECIALIST TRAINING PROGRAM THAT WILL PREPARE A WORKFORCE TO ADDRESS BOTH CHRONIC HEALTH CONDITIONS AND PROVIDE PEER RECOVERY SUPPORT THROUGH LIVED EXPERIENCE. THE GOALS OF THE PROJECT ARE TO 1) EXPAND THE PUBLIC HEALTH WORKFORCE TO INCLUDE THE NEW DUAL CERTIFICATION IN CHW-CPRS, 2) PROVIDE ENHANCED SKILL TRAINING TO CURRENT CHWS AND CPRSS, 3) INCREASE EMPLOYMENT READINESS THROUGH PLACEMENT IN APPRENTICESHIP PROGRAMS AND EXPERIENTIAL TRAINING, AND 4) ENHANCE HEALTH EQUITY IN MEDICALLY UNDERSERVED COMMUNITIES OF SOUTHERN NEW JERSEY BY BUILDING AWARENESS OF EMPLOYMENT OPPORTUNITIES AND PROVIDING JOB PLACEMENT ASSISTANCE. INNOVATIVE ACTIVITIES INCLUDE A PARTNERSHIP WITH BOTH THE NEW JERSEY DEPARTMENT OF HEALTH AND THE DEPARTMENT OF HUMAN SERVICES TO IMPLEMENT THE PROGRAM, THE DEVELOPMENT OF A NEW JERSEY DUAL CHW-CPRS COLLABORATIVE THAT INCLUDES STATE LEADERS AND INDIVIDUALS WITH LIVED EXPERIENCE TO OVERSEE THE PROGRAM, DEVELOPMENT OF AN APPRENTICESHIP MODEL WITHIN A SUBSTANCE USE TREATMENT AGENCY TO DEMONSTRATE NEW JOB FUNCTIONS OF THE DUALLY CERTIFIED WORKFORCE, EXPANDING EXPERIENTIAL TRAINING TO INCLUDE MOBILE OUTREACH UNITS, CREATING A N

IMPACT AID PROGRAM TITLE VIII SECTION 8003

IMPACT AID PROGRAM TITLE VIII SECTION 8003

MINORITY AIDS INITIATIVE FOR HIGH RISK MEN OF NEW JERSEY

IMPACT AID PROGRAM TITLE VIII SECTION 8003 AND SECTION 8007(A)

HIV AND COCAINE USE LEADS TO LOSS OF ASTROCYTE NEUROTROPHIC SUPPORT AND IMPAIRED LIPID HOMEOSTASIS IN THE BRAIN - SUMMARY TIGHT METABOLIC COUPLING BETWEEN ASTROCYTES AND NEURONS INVOLVES ASTROCYTES SENSING NEURONAL STRESS AND RESPONDING BY TAKING UP LIPID-LIKE PARTICLES CONTAINING EXCESS PEROXIDATED FATTY ACIDS (FA) GENERATED DURING STRESS. DURING STRESSFUL CONDITIONS, THE GENERATION OF REACTIVE OXYGEN SPECIES (ROS) INDUCE THE PEROXIDATION OF FA IN NEURONS. NEURONS ARE HIGHLY SENSITIVE TO TOXIC PEROXIDATED FA AND UNLIKE ASTROCYTES, NEURONS HAVE A LOW CAPACITY TO FORM LIPID DROPLETS (LD) TO ENCASE THE TOXIC FA. MOREOVER, NEURONAL MITOCHONDRIA ARE UNABLE TO EFFICIENTLY CONSUME FAS AS AN ENERGY SOURCE. THUS, NEURONS EXPEL LIPID-LIKE PARTICLES CARRYING THE FAS. ASTROCYTES ENDOCYTOSE LIPID-LIKE PARTICLES WITH FA, DELIVER THEM TO THE ER FOR PACKAGING INTO LIPID DROPLETS (LD) TO PROTECT THE CELL FROM THE TOXIC FAS. LD ALSO PROVIDE A CONDUIT FOR DELIVERY OF FA TO ASTROCYTE MITOCHONDRIA FOR USE AS AN ALTERNATIVE ENERGY SOURCE DURING STRESS. IN NORMAL CONDITIONS, ASTROCYTES USE GLUCOSE RATHER THAN FA AS THEIR MAIN SOURCE OF RESERVE ENERGY UNDER NORMAL CONDITIONS. METABOLIC COORDINATION BETWEEN ASTROCYTES AND NEURONS IS CRITICAL FOR CNS FUNCTIONING AND LIPID HOMEOSTASIS. HOWEVER, CHANGES IN ASTROCYTE-NEURON COUPLING FOR LIPID METABOLISM IN RESPONSE TO HIV AND COCAINE USE IS UNKNOWN. IT IS KNOWN THAT TOXIC, PEROXIDATED FATTY ACIDS (FAS) PRODUCED AND EXPELLED BY STRESSED NEURONS ARE TRANSFERRED TO ASTROCYTIC LIPID DROPLETS (LD) BY LIPOPROTEIN PARTICLES. ASTROCYTES CONSUME THE FAS STORED IN LD VIA MITOCHONDRIAL SS-OXIDATION. THUS, METABOLISM OF NEURON-DERIVED FA METABOLISM BY ASTROCYTES PROTECTS NEURONS FROM FA TOXICITY. DISRUPTION OF THIS TIGHTLY COORDINATED COUPLING TO METABOLIZE FAS LIKELY CONTRIBUTES TO THE INCREASED ASTROCYTIC ENERGY METABOLISM AND NEURONAL DEFICIT REPORTED DURING DETRIMENTAL SYNERGY BETWEEN HIV INFECTION AND COCAINE USE.

HEDGEHOG SIGNALING REGULATION OF POSTNATAL TONGUE AND TASTE ORGAN MORPHOGENESIS - ABSTRACT THE TONGUE HAS A MAJOR DEVELOPMENTAL TRAJECTORY DURING SUCKLING AND WEANING PERIODS AS THEY ADAPT FOR CHANGES IN FEEDING PATTERNS AND MOVEMENT FOR SPEECH. THIS GROWTH ENCOMPASSES NUMEROUS AND EXTREME CHANGES, INCLUDING INCREASES IN ANTERIOR TONGUE TASTE ORGAN FUNGIFORM PAPILLA (FP) TASTE CELL NUMBER AND SIZE, CONICAL KERATINIZATION OF NON-TASTE FILIFORM PAPILLAE (FILIFP), THICKENING OF STROMA AND BIOCHEMICAL CHANGES IN MUSCLES. HOWEVER, KNOWLEDGE GAPS REMAIN REGARDING THE MOLECULAR MECHANISMS ORCHESTRATING THIS GROWTH IN DIFFERENT ANATOMICAL COMPARTMENTS OF THE POSTNATAL TONGUE. HEDGEHOG (HH) SIGNALING IS VITAL FOR EMBRYONIC TONGUE DEVELOPMENT AND TASTE ORGAN MAINTENANCE IN ADULTS, BUT ITS ROLE IN POSTNATAL TONGUE IS NOT CLEARLY ELUCIDATED. WE HAVE STRIKINGLY DISCOVERED THAT IT EXTENDS BEYOND FP IN THE EARLY POSTNATAL TONGUE, SPECIFICALLY WITHIN FILIFP, SUGGESTING A POTENTIAL ROLE FOR HH SIGNALING IN FILIFP MATURATION. TO CHALLENGE THE CURRENT PARADIGM, WE WILL IDENTIFY HH PATHWAY COMPONENTS AND HH-REGULATED TARGET GENES IN EARLY POSTNATAL FILIFP EPITHELIUM USING RNA AND DNA SEQUENCING TOOLS (AIM 1A). WE WILL THEN DESCRIBE HOW HH SIGNALING AND ITS CANDIDATE FILIFP-SPECIFIC TARGET GENES CONTRIBUTE TO POSTNATAL FILIFP MATURATION AND FUNCTION BY USING AN EPITHELIUM-SPECIFIC GENETIC MOUSE MODEL TO INHIBIT THE PATHWAY (AIM 1B). FURTHER, HH RECEPTORS CDON, GAS1 AND PTCH1, DESPITE THEIR KNOWN RELEVANCE IN OTHER POSTNATAL TISSUES, REMAIN UNINVESTIGATED IN THE POSTNATAL TONGUE. WE OBSERVE THAT THEIR EXPRESSION IS LARGELY NON-OVERLAPPING IN FP AND FILIFP EPITHELIUM, STROMA OR LINGUAL MUSCLES DURING THE FIRST POSTNATAL WEEK. IMPORTANTLY, THEIR EXPRESSION PATTERNS SHIFT IN COORDINATION WITH TONGUE AND TASTE ORGAN MATURATION BY POSTNATAL DAY 21. WE HYPOTHESIZE THAT THE DISTINCT SPATIOTEMPORAL EXPRESSION OF HH RECEPTORS IN THE EARLY POSTNATAL TONGUE DIFFERENTIALLY REGULATES MORPHOGENESIS IN DIFFERENT ANATOMICAL COMPARTMENTS AND RESTRICTS THEIR FUNCTION IN LATE POSTNATAL STAGES TO MAINTAINING TASTE ORGANS. THEREFORE, OUR SECOND AIM WILL EMPLOY EPITHELIUM-, STROMA- OR MUSCLE-SPECIFIC CONDITIONAL LOSS-OF-FUNCTION APPROACHES FOR CDON, GAS1 OR PTCH1 TO ELUCIDATE THEIR FUNCTIONS IN FEEDING AND TASTE. WE WILL DESCRIBE THE INDIVIDUAL CONTRIBUTIONS OF CDON, GAS1 OR PTCH1 TO THE GROWTH OF FP AND FILIFP (AIM 2A), STROMA (AIM 2B) AND MUSCLE (AIM 2C) ACROSS EARLY AND LATE POSTNATAL STAGES. OUR GOALS ARE TO UNVEIL THE ROLE OF HH SIGNALING AND ITS ESSENTIAL CELL SURFACE RECEPTORS IN THE POSTNATAL DEVELOPMENT OF THE LINGUAL EPITHELIUM, STROMA AND MUSCLES, AND TO ELUCIDATE THE FUNCTIONAL CONSEQUENCES OF HH PATHWAY/RECEPTOR INHIBITION ON THE JUVENILE FEEDING AND TASTE SYSTEM. THESE FINDINGS WILL PROVIDE A MOLECULAR UNDERSTANDING OF TONGUE MATURATION AFTER BIRTH ACROSS DIFFERENT ANATOMICAL COMPARTMENTS, LAYING THE GROUNDWORK FOR IMPROVED DIAGNOSTIC AND TREATMENT STRATEGIES FOR PEDIATRIC TONGUE-RELATED ABNORMALITIES.

MODEL STATE-SUPPORTED AREA HEALTH EDUCATION CENTERS

ANTIMICROBIAL MECHANISMS OF SPECIALIZED PRORESOLVING MEDICATORS

BLOOD-BASED BIOMARKERS FOR EARLY DETECTION OF ALZHEIMER'S DISEASE

PROJECT CARES: INCREASING ACCESS TO EFFECTIVE TRAUMA-FOCUSED TREATMENT THROUGH TRAINING AND SELF-CARE - PROJECT CARES: INCREASING ACCESS TO EFFECTIVE TRAUMA-FOCUSED TREATMENT THROUGH TRAINING AND SELF-CARE SEEKS TO INCREASE THE AVAILABILITY OF EVIDENCE-BASED THERAPY SERVICES FOR THE RACIALLY, ETHNICALLY, AND SOCIOECONOMICALLY DIVERSE YOUTH WHO HAVE EXPERIENCED TRAUMA IN THE URBAN, SUBURBAN, AND RURAL AREAS OF SOUTHERN NEW JERSEY (NJ) AS WELL AS OTHER REGIONS NATIONWIDE. THIS PROJECT WILL IMPLEMENT TWO PROGRAMS TO ACHIEVE THIS GOAL. FIRST, THE GRANT WILL SUPPORT A FELLOWSHIP PROGRAM THAT WILL RECRUIT AND TRAIN 16 POSTGRADUATE MENTAL HEALTH PROFESSIONALS (FOUR EACH YEAR IN YEARS TWO THROUGH FIVE) TO PARTICIPATE IN ONE-YEAR FULL-TIME PAID TRAINING EXPERIENCES. FELLOWS WILL PROVIDE DIRECT SERVICES AND ENGAGE IN PROFESSIONAL DEVELOPMENT ACTIVITIES, INCLUDING PARTICIPATION IN FOUR MULTIDISCIPLINARY TEAM MEETINGS, TWO TRAUMA-FOCUSED PRESENTATIONS TO CHILD PROTECTIVE SERVICES, AND MONTHLY DIVERSITY, EQUITY, AND INCLUSION MEETINGS. CARES WILL CREATE AND REFINE THE MATERIALS, RESOURCES, AND CURRICULUM FOR THE FELLOWSHIP PROGRAM AND MAKE THEM AVAILABLE TO OTHER ORGANIZATIONS THAT WANT TO CREATE SIMILAR PROGRAMS. THE FELLOWS AND THEIR SUPERVISORS WILL DIRECTLY SERVE 438 YOUTH (AGES 3 TO 18 YEARS) AND THEIR NONOFFENDING CAREGIVERS IN THE SEVEN SOUTHERN COUNTIES OF NJ DURING THE GRANT PERIOD (30 IN YEAR ONE, 102 EACH YEAR IN YEARS TWO THROUGH FIVE) THROUGH EVIDENCE-BASED ENGAGEMENT STRATEGIES AND THE PROVISION OF TF-CBT VIA TELEHEALTH AND IN-PERSON WITH A SUCCESSFUL COMPLETION RATE OF AT LEAST 75 PERCENT. YOUTH WHO COMPLETE TF-CBT WILL DEMONSTRATE REDUCTIONS IN POSTTRAUMATIC STRESS AND BEHAVIOR PROBLEMS. FELLOWS WILL DEMONSTRATE FIDELITY TO THE TF-CBT MODEL. SECOND, THE GRANT WILL FUND THE REFINEMENT AND DELIVERY OF AN EVIDENCE-BASED SELF-CARE INTERVENTION, REFERRED TO AS PRACTICE WHAT YOU PREACH, TO 160 (40 EACH YEAR IN YEARS ONE THROUGH FOUR) CLINICIANS, SUPERVISORS, AND SENIOR LEADERS IN NJ AND, IN CONJUNCTION WITH OUR NCTSI II PARTNERS, ACROSS THE NATION. PARTICIPANTS WILL DEMONSTRATE ENHANCED COPING SKILLS AND TF-CBT COMPETENCY AND FIDELITY, AND REDUCTIONS IN SECONDARY TRAUMATIC STRESS (STS) AND TURNOVER INTENTION, WHICH IS PARTICULARLY CRUCIAL AT THIS TIME GIVEN THAT THE PANDEMIC HAS ADDED SIGNIFICANT ADDITIONAL STRESS AND NECESSITATED A NEW SERVICE DELIVERY FORMAT (I.E., TELEHEALTH). RECENT RESEARCH FINDINGS SUGGEST THAT THE PROPOSED SELF-CARE INTERVENTION IS ASSOCIATED WITH INCREASES IN CLINICIANS’/SUPERVISORS’ USE OF THE EFFECTIVE COPING SKILLS AND REDUCTIONS IN STS. GIVEN THAT STS CAN IMPAIR CLINICAL EFFECTIVENESS AND LEAD TO HIGH STAFF TURNOVER RATES, THIS SELFCARE INTERVENTION HAS THE POTENTIAL TO GREATLY REDUCE TURNOVER AND ENHANCE THE EFFECTIVENESS OF TREATMENT SERVICES DELIVERED. IN SUM, BOTH THE FELLOWSHIP PROGRAM, THROUGH ITS CUTTING-EDGE TRAINING, ENGAGEMENT, AND DELIVERY OF DIRECT SERVICES AND THE SELF-CARE INTERVENTION, WHICH AIMS TO IMPROVE COPING, DECREASE STS, AND INCREASE RETENTION OF TRAUMA PROFESSIONALS IN THE FIELD, WILL ULTIMATELY ENHANCE THE QUALITY AND ACCESSIBILITY OF EVIDENCE-BASED SERVICES FOR DIVERSE POPULATIONS OF YOUTH AND CAREGIVERS IMPACTED BY TRAUMA IN NJ AS WELL AS OTHER PARTS OF THE NATION.

HETEROGENEOUS PROPERTIES OF LC EFFERENTS TO MODALITY-SPECIFIC TERMINAL FIELDS

DIFFERENTIAL CLEARANCE OF PYROGLUTAMATE ABETA THROUGH ARACHNOID AND MENINGEAL LYMPHATICS IN ALZHEIMER DISEASE

ALASKA NATIVE EDUCATIONAL PROGRAM - ALASKA NATIVE EDUCATION

FATTY ACIDS-MEDIATED INFLAMMATION AND ETHNIC DISPARITY IN PREGNANCY OUTCOME

RPA-DIRECTED DNA REPAIR MECHANISMS - PROJECT SUMMARY REPLICATION PROTEIN A (RPA) IS REQUIRED FOR NEARLY EVERY DNA REPAIR AND REPLICATION PROCESS. RPA BINDS SINGLE- STRANDED DNA AND INTERACTS WITH DOZENS OF PROTEINS AT SITES OF DNA MAINTENANCE. WE ARE INTERESTED IN THE PROTEIN COMPLEXES THAT FORM BETWEEN RPA AND OTHER PROTEINS WHEN PERFORMING SPECIFIC DNA REPAIR TASKS. THE CURRENT PROJECT EXAMINES RPA’S ROLE AS A CENTRAL SCAFFOLD IN URACIL BASE EXCISION REPAIR. OUR APPROACH IS TO MANIPULATE THE BINDING OF RPA TO URACIL DNA GLYCOSYLASE (UNG2) TO EXAMINE THE RELEVANCE OF THEIR INTERACTION. WE HAVE DEVELOPED A STRATEGY TO COVALENTLY TETHER TOGETHER RPA AND UNG2 TO FORM MINI DNA REPAIR COMPLEXES THAT RESEMBLE THE ARCHITECTURE OF THE TWO PROTEINS WHEN THEY INTERACT IN CELLS. OUR PRELIMINARY DATA DEMONSTRATES OUR SUCCESS AT FORMING RPA-UNG2 PROTEIN COMPLEXES IN BOTH CELLULAR AND RECOMBINANT SYSTEMS. THIS ALLOWS US TO DEFINITIVELY DETERMINE HOW RPA FUNCTIONS AS PART OF A PROTEIN COMPLEX WITH UNG2, AND COMPLEMENTARY STUDIES EXAMINE THE EFFECTS OF WEAKENING THEIR ASSOCIATION. SPECIFICALLY, AIM 1 USES PURIFIED RPA AND UNG2 CONSTRUCTS TO EXAMINE THE ACTIVITY OF THE PROTEINS ALONE OR AS PART OF A COMPLEX. BINDING AND ENZYMATIC EXPERIMENTS USING SYNTHETIC DNA SUBSTRATES WILL DETERMINE THE STRUCTURAL NATURE OF THEIR SUBSTRATES IN VIVO. WE FOCUS SPECIFICALLY ON THE ACTION OF RPA AND UNG2 AT SSDNA-DSDNA JUNCTIONS, THEIR KNOWN SUBSTRATES, BY PREPARING URACILATED FORK-LIKE DNA STRUCTURES THAT RESEMBLE REPLICATION FORKS FOUND IN THE NUCLEUS. AIM 2 EXAMINES THE RPA-UNG2 PROTEIN COMPLEX IN HUMAN COLORECTAL CELLS UNDER CONDITIONS OF URACIL STRESS THAT ARE INDUCED BY COMMONLY USED CHEMOTHERAPEUTIC AGENTS. THE STRENGTH OF RPA’S INTERACTION WITH UNG2 WILL BE CONTROLLED AND CORRELATED WITH UNG2’S URACIL EXCISION EFFICIENCY AT THE REPLICATION FORK. FINALLY, ADDITIONAL CELLULAR PROTEINS THAT ASSOCIATE WITH RPA AND UNG2 WILL BE IDENTIFIED. THE FUNCTION OF THESE PROTEINS WILL BE EXAMINED IN THE CONTEXT OF MULTI-PROTEIN COMPLEXES THAT FORM DURING URACIL BASE EXCISION REPAIR. THE TARGETED SCOPE OF THIS PROJECT EXAMINING RPA IN BASE EXCISION REPAIR WILL FACILITATE OUR METHODOLOGICAL DEVELOPMENT, WHICH WILL BE WIDELY ADAPTABLE FOR EXAMINING RPA-CONTAINING PROTEIN COMPLEXES IN OTHER DNA REPAIR PROCESSES.

THE ROLE OF SUPEROXIDE DISMUTASE SOD-1 IN MICROBE-GUT-BRAIN INTERACTION

MONITORING MECHANISMS IN MAMMALIAN RIBOSOME BIOGENESIS

ALASKA NATIVE EDUCATION

ROLE OF BRAIN AVPR1A-EXPRESSING NEURONS IN MODULATION OF SOCIAL BEHAVIOR - PROJECT SUMMARY THE IMPORTANCE OF UNDERSTANDING THE NEUROBIOLOGY OF SOCIAL BEHAVIOR IS UNDERSCORED BY THE FACT THAT SOCIAL INTERACTIONS PERMEATE NEARLY EVERY ASPECT OF HUMAN LIFE. HOW WE ARE RAISED, OUR INTERACTIONS WITH PEERS, ROMANTIC RELATIONSHIPS, PARENTING OUR OWN CHILDREN. ALL THESE RELATIONSHIPS IMPACT OUR QUALITY OF LIFE, AND ATYPICAL SOCIAL INTERACTIONS CAN HAVE DEVASTATING CONSEQUENCES BOTH FOR INDIVIDUALS AND THEIR SOCIAL NETWORKS. THE NEGATIVE IMPACT OF DISRUPTED SOCIAL BEHAVIOR CAN LEAD TO AND EXACERBATE HEALTH PROBLEMS, WHEREAS POSITIVE SOCIAL INTERACTIONS AND SUPPORTIVE ENVIRONMENTS CAN IMPROVE HEALTH OUTCOMES. BETTER UNDERSTANDING OF NEURAL MECHANISMS UNDERLYING SOCIAL BEHAVIOR, A KEY OBJECTIVE OF STRATEGIC GOALS LAID OUT BY THE NATIONAL INSTITUTE OF MENTAL HEALTH, WILL HELP IDENTIFY TARGETS FOR TREATMENT OF UNDERLYING CAUSES THAT DISRUPT BEHAVIOR AND MOTIVATE NEW WAYS OF USING SOCIAL INTERACTION ITSELF AS A THERAPEUTIC INTERVENTION TO IMPROVE HEALTH OUTCOMES. DECADES OF RESEARCH HAVE SHOWN UNEQUIVOCALLY THAT THE NEUROPEPTIDE ARGININE VASOPRESSIN (AVP) IMPACTS BOTH PRO- SOCIAL (E.G., PARENTAL CARE, JUVENILE PLAY, PAIR-BONDING, AND SOCIAL MEMORY) AND ANTI-SOCIAL (E.G., AGGRESSION AND TERRITORIAL FLANK-MARKING) BEHAVIORS IN NUMEROUS MAMMALIAN SPECIES, BUT MECHANISMS BY WHICH AVP IMPACTS SOCIAL BEHAVIOR ARE NOT FULLY UNDERSTOOD CREATING A CRITICAL GAP IN KNOWLEDGE. ANATOMICAL STUDIES OF AVP NEURON PROJECTIONS SUGGEST THAT AVP PRODUCING NEURONS IN THE BED NUCLEUS OF THE STRIA TERMINALIS (BNST) AND MEDIAL AMYGDALA ARE LIKELY TO BE INVOLVED IN THE REGULATION OF PROSOCIAL BEHAVIOR GIVEN THAT THEIR TARGETS INCLUDE BRAIN REGIONS ASSOCIATED WITH MOTIVATED BEHAVIOR, EMOTIONAL STATE, AND REWARD (E.G., THE MEDIODORSAL THALAMUS, THE TARGET OF PROPOSED STUDIES). A CENTRAL HYPOTHESIS IS THAT ACTIVATION OF AVP-RESPONSIVE (I.E., AVP 1A RECEPTOR- EXPRESSING, AVPR1A) NEURONS IN BRAIN REGIONS CONTROLLING MOTIVATION AND EMOTION PROMOTE PRO-SOCIAL BEHAVIOR (I.E., INCREASE INTEREST IN SOCIAL STIMULI AND ENGAGEMENT IN NON-AGGRESSIVE BEHAVIORS) AND DECREASE ANTI-SOCIAL BEHAVIOR (I.E., SOCIAL AVOIDANCE AND DISPLAY OF AGGRESSIVE BEHAVIORS). TO TEST THIS HYPOTHESIS AND DISCOVER UNDERLYING MECHANISMS, 3 AIMS ARE PROPOSED. PROPOSED EXPERIMENTS MAKE USE OF A NEWLY CHARACTERIZED MOUSE MODEL THAT ALLOWS GENETIC ACCESS TO AVPR1A-EXPRESSING CELLS ENABLING SELECTIVE TARGETING AND DIFFERENTIATION BETWEEN AVPR1A AND NON-AVPR1A NEURONS. IN AIM 1, ELECTROPHYSIOLOGICAL, TRANSCRIPTOMIC, AND VIRAL TRACING STRATEGIES WILL BE USED TO IDENTIFY THE FUNDAMENTAL ELECTRICAL BEHAVIOR, GENE EXPRESSION PATTERNS, AND CONNECTIVITY OF MEDIODORSAL THALAMUS AVPR1A NEURONS, A PREVIOUSLY UNEXPLORED NEURONAL SUBTYPE. THE MEDIODORSAL THALAMUS IS A DOWN STREAM TARGET OF THE DOPAMINE REWARD SYSTEM, IS INNERVATED BY THE BNST AVP SYSTEM, AND THROUGH ITS PROJECTIONS TO THE MEDIAL PREFRONTAL CORTEX INFLUENCES MEMORY, COGNITIVE FLEXIBILITY, AND DECISION-MAKING, ALL CRITICAL TO THE EXPRESSION OF SOCIAL BEHAVIORS. THEN, A CHEMOGENETIC LOSS-OF-FUNCTION STRATEGY WILL BE USED TO DIRECTLY ASSESS THE ROLE OF THE MEDIODORSAL THALAMUS AVPR1A NEURON SUBTYPE DURING SOCIAL INTERACTIONS (AIM 2) AND TESTS OF COGNITIVE BEHAVIOR (AIM 3).

PRIMARY CARE TRAINING AND ENHANCEMENT - LANGUAGE AND DISABILITY ACCESS - THE ROWAN INTEGRATED SPECIAL NEEDS (RISN) CENTER AT ROWAN SCHOOL OF OSTEOPATHIC MEDICINE PROPOSES TO TRAIN 90 RESIDENTS AND 1,440 MEDICAL STUDENTS OVER FIVE YEARS USING A NEW CURRICULUM FOCUSED ON THE CARE OF INDIVIDUALS WITH PHYSICAL DISABILITIES AND INTELLECTUAL AND DEVELOPMENTAL DISABILITIES THAT INCLUDES DIDACTIC TRAINING, CLINICAL ROTATIONS, AND COMMUNITY SERVICE LEARNING. THE ROWAN INTEGRATED SPECIAL NEEDS (RISN) CENTER AT ROWAN UNIVERSITY SCHOOL OF OSTEOPATHIC MEDICINE (ROWANSOM) IS SOUTH JERSEY'S FIRST PRIMARY CARE PRACTICE DEDICATED TO SERVING THE NEEDS OF INDIVIDUALS WITH AUTISM, BRAIN INJURY, DOWN SYNDROME, INTELLECTUAL DISABILITY, CEREBRAL PALSY, AND OTHER COMPLEX CONDITIONS ORIGINATING IN CHILDHOOD. THE PROJECT WILL USE RISN AS A TRAINING SITE TO INCREASE THE NUMBER OF RESIDENTS AND MEDICAL STUDENTS TRAINED TO PROVIDE CULTURALLY COMPETENT AND LINGUISTICALLY APPROPRIATE CARE AND SERVICES TO INDIVIDUALS WITH PHYSICAL DISABILITIES AND/OR IDD. THE GEOGRAPHIC CATCHMENT AREA WILL BE FOUR COUNTIES IN NEW JERSEY, INCLUDING CAMDEN, CUMBERLAND, GLOUCESTER, AND SALEM, WITH OVER 134,000 INDIVIDUALS LIVING WITH IDD. THE POPULATION OF FOCUS WILL BE OSTEOPATHIC MEDICAL STUDENTS AT ROWANSOM, PRIMARY CARE RESIDENTS AT INSPIRA HEALTH (LOCATED IN RURAL CUMBERLAND COUNTY), AND OSTEOPATHIC PHYSICIANS ON THE FACULTY OF ROWANSOM AND OTHER MEDICAL SCHOOLS. THE GOAL OF THE TRAINING IS TO INCREASE ACCESS TO QUALITY PRIMARY CARE SERVICES FOR INDIVIDUALS WITH PHYSICAL DISABILITIES AND/OR IDD. OBJECTIVES INCLUDE 1) INCREASE THE NUMBER OF OSTEOPATHIC MEDICAL STUDENTS, RESIDENTS, AND FACULTY THAT ARE TRAINED TO PROVIDE CULTURALLY AND LINGUISTICALLY APPROPRIATE CARE TO INDIVIDUALS WITH PHYSICAL DISABILITIES AND IDD, 2) DEVELOP AND IMPLEMENT A CULTURALLY COMPETENT DIDACTIC AND CLINICAL TRAINING CURRICULUM FOR THE STUDENTS, FACULTY, AND PRECEPTORS, AND 3) PROVIDE OPPORTUNITIES FOR CLINICAL TRAINING WHERE MEDICAL STUDENTS AND MEDICAL RESIDENTS WILL HAVE DIRECT CONTACT WITH PEOPLE WITH PHYSICAL AND INTELLECTUAL AND DEVELOPMENTAL DISABILITIES IN RURAL AND UNDERSERVED COMMUNITIES. INNOVATIONS INCLUDE PROVIDING TRAINING IN THE RISN CENTER, WHICH PROVIDES INTEGRATED PRIMARY CARE AND BEHAVIORAL SERVICES USING A MEDICAL HOME MODEL FOR PEOPLE WITH PHYSICAL DISABILITIES AND IDD; DEVELOPMENT OF CORE COMPETENCIES FOR OSTEOPATHIC MEDICAL STUDENTS AND RESIDENTS IN THE CARE OF PEOPLE WITH PHYSICAL DISABILITIES AND IDD; INTERFACING WITH THE NEW JERSEY AHEC PROGRAM AND COMMUNITY SERVICE LEARNING CURRICULUM TO PROVIDE EXPERIENTIAL TRAINING IN MEDICALLY UNDERSERVED COMMUNITIES, INTEGRATING SOCIAL DETERMINANTS OF HEALTH INTO CLINICAL AND EXPERIENTIAL TRAINING, AND OTHER ACTIVITIES. A PROCESS AND OUTCOME EVALUATION FOR THE PROJECT IS PLANNED ALONG WITH A LONGITUDINAL STUDY OF MEDICAL STUDENTS AND RESIDENTS WHO PARTICIPATED IN THE TRAINING PRACTICING IN RURAL AND MEDICALLY UNDERSERVED AREAS ONE YEAR AFTER GRADUATION.

TRANSLATIONAL RESCUE MECHANISMS IN EUKARYOTES

EVOLUTION OF DEVELOPMENTAL REGULATORY PATHWAYS

AFFORDABLE CARE ACT: PRIMARY CARE RESIDENCY EXPANSION

GERIATRIC TRAINING PROGRAM FOR PHYSICIANS, DENTISTS, AND BEHAVIORAL AND MENTAL HEALTH PROFESSIONS

STRESS REGULATED MITOCHONDRIAL DYNAMICS

MECHANISMS OF TRANSIENT TRANSCRIPTION IN YEAST

DISSECTING THE ORIGINS OF A COMPLEX REPRODUCTIVE TRAIT: NEMATODE SELF FERTILITY

REGULATION OF THE GLI PROTEIN TRA-1 BY CO-FACTORS

CAROL M. WHITE PHYSICAL EDUCATION PROGRAM

IMPACT OF NIGRAL AND EXTRANIGRAL NEURODEGENERATION ON AERODIGESTIVE DISCOORDINATION IN A PESTICIDE MODEL OF PARKINSON'S DISEASE - PROJECT SUMMARY SWALLOWING PROBLEMS IN PARKINSON’S DISEASE (PD) AFFECT 90% OF PATIENTS, WITH SIGNIFICANT CONSEQUENCES FOR QUALITY OF LIFE AND MORBIDITY, AND ARE RESISTANT TO CURRENT DOPAMINE DEFICIENCY TARGETING TREATMENTS FOR MOVEMENT IMPAIRMENTS CAUSED BY PD (COELHO ET AL., 2010; PLOWMAN-PRINE ET AL., 2009). THE TREATMENT RESISTANT NATURE OF SWALLOWING PROBLEMS IMPLICATES NEUROLOGICAL DAMAGE OUTSIDE THE SUBSTANTIA NIGRA AND STRIATUM. MOST CASES OF PD RESULT IN PART FROM EXPOSURE TO TOXIC SUBSTANCES, RESULTING IN WIDESPREAD BRAIN PATHOLOGIES (DE LAU & BRETELER, 2006). PD AFFECTS MULTIPLE COMPONENTS OF FEEDING AND THEIR COORDINATION, WHICH ARE CONTROLLED BY DIFFERENT PARTS OF THE BRAIN (KWON &LEE, 2019). THE OVERALL GOAL OF THIS PROJECT IS TO IDENTIFY TREATMENT RESISTANT COMPONENTS OF FEEDING AND THEIR NEUROPATHOLOGICAL CORRELATES IN PD. THE CENTRAL HYPOTHESIS IS THAT SPECIFIC COMPONENTS OF THE FEEDING PROCESS ARE DIFFERENTIALLY IMPAIRED BY NEURODEGENERATION INSIDE AND OUTSIDE THE SUBSTANTIA NIGRA RESULTING IN AN OVERALL TREATMENT RESISTANT PHENOTYPE. THREE RELATED SPECIFIC AIMS WILL TEST THIS HYPOTHESIS. SPECIFIC AIMS 1 WILL COMPARE BEHAVIORAL, BIOMECHANICAL, AND NEUROMUSCULAR DIFFERENCES IN CHEW-SWALLOW-BREATHE COORDINATION IN A GENERALIZED MODEL OF PD, THE RAT ROTENONE INJECTION MODEL (CANNON ET AL., 2009) VERSUS A 6-OHDA INDUCED TARGETED NIGROSTRIATAL LESION MODEL (RUSSELL ET AL., 2013) TO IDENTIFY FEEDING DYSFUNCTION NOT RELATED TO NIGROSTRIATAL DEFICITS. SPECIFIC AIM TWO WILL EXAMINE HOW DIFFERENCES IN BEHAVIORAL AND SENSORIMOTOR COMPLEXITY BETWEEN SOLID FOOD EATING AND LIQUID DRINKING AFFECT SEVERITY OF BEHAVIORAL, BIOMECHANICAL, AND NEUROMUSCULAR OROPHARYNGEAL DISCOORDINATION IN A ROTENONE INJECTION RAT MODEL OF PD. SPECIFIC AIM 3 WILL TEST THE BEHAVIORAL, BIOMECHANICAL, AND NEUROMUSCULAR IMPACT OF LEVODOPA-INDUCED RESCUE OF CHEWING FUNCTION (KARLSSON ET AL., 1992) ON FEEDING COORDINATION IN A ROTENONE INJECTION RAT MODEL OF PD TO IDENTIFY PATHOPHYSIOLOGICAL MECHANISMS OF DYSPHAGIA THAT ARE RESISTANT TO TREATMENTS TARGETING DOPAMINE DEFICIENCY. THE APPROACH IS INNOVATIVE IN COMBINING COMPLIMENTARY LESION MODELS, BEHAVIORAL MODELS, AND NEUROPHARMACOLOGICAL APPROACHES, WITH A DETAILED ANALYSIS OF FEEDING TO FIND THE PATHOPHYSIOLOGICAL BASIS OF DYSPHAGIA TREATMENT RESISTANCE IN PD. THE APPROACH IS SIGNIFICANT IN TARGETING THE UNDERLYING MECHANISM OF TREATMENT RESISTANCE IN A COMMON, UNDERSTUDIED SYMPTOM OF PD. THE NEUROMUSCULAR, NEUROPATHOLOGICAL, AND BIOMECHANICAL FINDINGS OF THIS FROM THIS WORK WILL LEAD TO FURTHER RESEARCH INTO THE NEUROLOGICAL BASIS OF TREATMENT RESISTANCE OF DYSPHAGIA IN PD, WITH THE ULTIMATE GOAL OF DEVELOPING TARGETED THERAPEUTIC INTERVENTIONS.

GERIATRIC EDUCATION CENTERS

THE PROJECT TITLE IS: NEW JERSEY COLLABORATIVE TO IMPROVE ACCESS TO OVERDOSE TREATMENT.

SUBSTANCE ABUSE AND HIV PREVENTION NAVIGATION FOR HIGH RISK AFRICAN AMERICAN AND HISPANIC YOUTH OF NEW JERSEY

TARGETING HEXOKINASE II IN CHEMOTHERAPY

ELEMENTARY AND SECONDARY SCHOOL COUNSELING

FUNCTIONAL NEUROANATOMY UNDERLYING PSYCHOSOCIAL STRESS-INDUCED COCAINE SEEKING

MITOCHONDRIAL MAGNESIUM REGULATES MCU ACTIVITY AND PTP OPENING DURING ISCHEMIC REPERFUSION INJURY - PROJECT SUMMARY MAGNESIUM (MG2+) IS A UBIQUITOUS INTRACELLULAR DIVALENT CATION AND A CRUCIAL CO-FACTOR IN THE MACHINERY THAT REPLICATES, TRANSCRIBES, AND TRANSLATES GENOMIC INFORMATION. LIKE CALCIUM, MG2+ IS COMPARTMENTALIZED TO MITOCHONDRIA, AND MRS2 IS THE ONLY KNOWN MOLECULAR MACHINERY ASSOCIATED WITH MITOCHONDRIAL MG2+ INFLUX. APART FROM ITS CONVENTIONAL FUNCTION OF ACTIVATING ENZYMES WITHIN THE TCA CYCLE AND ELECTRON TRANSPORT CHAIN, OUR PREVIOUS RESEARCH SHOWED MITOCHONDRIAL MG2+ (MMG2+) TO BIND THE N-TERMINAL DOMAIN OF THE MITOCHONDRIAL CA2+ UNIPORTER (MCU) REGULATING ACIDIC PATCH (MRAP) AND REGULATES MCU-MEDIATED MITOCHONDRIAL CA2+ (MCA2+) UPTAKE. THE OVERLOAD OF MCA2+ IS ASSOCIATED WITH HEART FAILURE (HF) AND BECAUSE MMG2+ REGULATES MCA2+ UPTAKE, WE ANTICIPATE A LOSS OF MMG2+ HOMEOSTASIS IN ISCHEMIC REPERFUSION (IR) INJURY, WHICH CAN OVERLOAD MCA2+ AND PREDISPOSE THE CARDIOMYOCYTES TO MITOCHONDRIAL PERMEABILITY TRANSITION PORE (MPTP)-MEDIATED CELL DEATH. OUR PRELIMINARY RESULTS INDICATED A SIGNIFICANT REDUCTION IN MMG2+ UPTAKE IN NRVMS EXPOSED TO HYPOXIA/REOXYGENATION RELATIVE TO THE CONTROL. INTRIGUINGLY, THE DECREASE IN MMG2+ WAS NOT DUE TO A DECREASE IN MRNA OR PROTEIN LEVELS, BUT RATHER POST-TRANSLATIONAL MODIFICATION OF MRS2 BY OXIDATION, RESULTING IN A LOSS OF FUNCTION. OUR PRELIMINARY RESULTS ALSO SHOWED THAT THE LOSS OF MMG2+ UPTAKE WAS RELATED TO INCREASED MCA2+ OVERLOAD, BIOENERGETICS CRISIS, AND MPTP OPENING. TO DETERMINE WHETHER IMPAIRED MMG2+ INFLUX CAUSES MCA2+ OVERLOAD AND MPTP OPENING, WE EXPRESSED A CYSTEINE NULL (MRS2C269A) MUTANT IN NRVMS. WE OBSERVED THAT CYSTEINE NULLIFICATION RENDERED MRS2 INSENSITIVE TO OXIDATION AND OXIDATIVE INHIBITION, PROTECTING NRVMS FROM MCA2+ OVERLOAD-MEDIATED CELL DEATH DURING HR INJURY. BASED ON THESE OBSERVATIONS, WE HYPOTHESIZE THAT OXIDATIVE STRESS INACTIVATES MRS2, RESULTING IN THE LOSS OF MMG2+ UPTAKE. THIS, IN TURN, RELIEVES MCU FROM MG2+- DEPENDENT NEGATIVE FEEDBACK INHIBITION, ULTIMATELY LEADING TO AN INCREASE IN MCU-MEDIATED MCA2+ OVERLOAD AND MAKING CARDIOMYOCYTES MORE SUSCEPTIBLE TO MPTP-MEDIATED CELL DEATH. TO EVALUATE WHETHER IMPAIRED MMG2+ INFLUX IS CAUSALLY LINKED TO MCA2+ OVERLOAD-MEDIATED CARDIOMYOCYTE DEATH AND HEART FAILURE, WE PROPOSE THREE AIMS: AIM 1: DEFINE THE MECHANISM BY WHICH POST-TRANSLATIONAL OXIDATIVE MODIFICATION REGULATES MRS2 ACTIVITY. AIM 2: INVESTIGATE THE REGULATORY ROLE OF MRS2 OXIDATION IN CARDIAC MITOCHONDRIAL BIOENERGETICS. AIM 3: DEFINE THE IONIC LINK BETWEEN MRS2 AND MCU IN REGULATING CARDIAC FUNCTION DURING IR INJURY. THE COMPLETION OF THE PROPOSED EXPERIMENTS WILL PROVIDE A MISSING LINK BETWEEN THE IONIC DYSREGULATION AND MITOCHONDRIAL DYSFUNCTION HYPOTHESES AND ADVOCATE TARGETING MITOCHONDRIAL ION HOMEOSTASIS AS A POWERFUL THERAPEUTIC STRATEGY TO INHIBIT OR REVERSE HF PROGRESSION, EVEN THOUGH THE ROLE OF MCA2+ OVERLOAD AND MPTP OPENING IN CARDIAC DISEASES ARE ALREADY KNOWN.

CARES ACT - HEERF INSTITUTION FUNDS

DO COCAINE AND CHRONIC STRESS CONVERGE IN THE BASOLATERAL AMYGDALA?

SUICIDE PREVENTION GRANTS ARE AWARDED TO ELIGIBLE ENTITIES TO MEET THE NEEDS OF ELIGIBLE INDIVIDUALS AND THEIR FAMILIES THROUGH OUTREACH, PROVISION OR COORDINATION OF SUICIDE PREVENTION SERVICES, AND CONNECTION TO VA AND COMMUNITY RESOURCES AS DESCRIBED IN 38 CFR PART 78 (HTTPS://WWW.ECFR.GOV/CURRENT/TITLE-38/CHAPTER-I/PART-78?TOC=1)

SUICIDE PREVENTION GRANTS ARE AWARDED TO ELIGIBLE ENTITIES TO MEET THE NEEDS OF ELIGIBLE INDIVIDUALS AND THEIR FAMILIES THROUGH OUTREACH, PROVISION OR COORDINATION OF SUICIDE PREVENTION SERVICES, AND CONNECTION TO VA AND COMMUNITY RESOURCES AS DESCRIBED IN 38 CFR PART 78 (HTTPS://WWW.ECFR.GOV/CURRENT/TITLE-38/CHAPTER-I/PART-78?TOC=1)

ENDOMETRIOSIS EDUCATION, SCREENING, BRIEF INTERVENTION, AND REFERRAL TO TREATMENT (ENDO-SBIRT) TO INCREASE DIAGNOSIS AND TREATMENT OF ENDOMETRIOSIS IN WOMEN WITH OUDS AT RISK FOR FATAL OVERDOSE - THE DEPARTMENT OF OBSTETRICS AND GYNECOLOGY AT ROWAN UNIVERSITY SCHOOL OF OSTEOPATHIC MEDICINE PROPOSES TO ENGAGE 225 WOMEN WITH OPIATE USE DISORDERS FROM UNDERSERVED COMMUNITIES OF NEW JERSEY IN AN ENDOMETRIOSIS EDUCATION, SCREENING, BRIEF INTERVENTION, AND REFERRAL TO TREATMENT (ENDO-SBIRT) INTERVENTION TO RAPIDLY IDENTIFY WOMEN AT RISK FOR ENDOMETRIOSIS, LINK THEM TO DIAGNOSIS AND TREATMENT, AND DECREASE FATAL OVERDOSE. ENDOMETRIOSIS AFFECTS APPROXIMATELY 10% OF WOMEN WORLDWIDE. THE CONDITION IS DEFINED BY THE PRESENCE OF ENDOMETRIAL-LIKE TISSUE OUTSIDE THE UTERINE CAVITY. IT IS RESPONSIBLE FOR SEVERE PAIN AND INFERTILITY AND HAS A MAJOR IMPACT ON QUALITY OF LIFE. THE DELAY BETWEEN THE ONSET OF SYMPTOMS AND DIAGNOSIS OF ENDOMETRIOSIS IS TYPICALLY BETWEEN 6 AND 10 YEARS. WHILE ENDOMETRIOSIS IS NOT FATAL, WOMEN WITH UNDIAGNOSED ENDOMETRIOSIS MAY TURN TO ILLICIT OPIATES TO RELIEVE PAIN, PLACING THEM AT RISK FOR OVERDOSE. A STUDY OF COMMERCIAL CLAIMS DATA FROM 2009 TO 2018 OF 61,019 WOMEN WITH ENDOMETRIOSIS FOUND THAT 37.9% HAD HIGH-RISK OR CHRONIC OPIATE USE CONDITIONS. THERE IS INCREASING EVIDENCE OF THE LINK BETWEEN CHRONIC PELVIC PAIN DUE TO UNADDRESSED ENDOMETRIOSIS AND THE TRANSITION FROM PRESCRIPTION OPIOIDS TO ILLICIT OPIATE USE. WHILE ADVANCES IN LAPAROSCOPIC SURGERY AND GYNECOLOGICAL IMAGING HAVE INCREASED THE ABILITY OF PHYSICIANS TO DIAGNOSE AND TREAT ENDOMETRIOSIS, REFERRING ALL PATIENTS SUSPECTED OF HAVING ENDOMETRIOSIS TO LAPAROSCOPIC SURGEONS AND SPECIALIZED RADIOLOGISTS IS NOT FEASIBLE. RESEARCHERS HAVE DEVELOPED A PATIENT QUESTIONNAIRE TO DETECT PATIENTS AT HIGH RISK FOR ENDOMETRIOSIS AS THE FIRST STEP IN THE DIAGNOSTIC PROCESS. HOWEVER, THE SCREENING TOOL HAS NEVER BEEN IMPLEMENTED IN A PUBLIC HEALTH MODEL TO ADDRESS DIAGNOSTIC DELAYS. GIVEN THE OPIATE OVERDOSE PUBLIC HEALTH CRISIS, THERE IS A NEED TO RAPIDLY IDENTIFY HIGH-RISK WOMEN WHO MAY BE USING ILLICIT OPIATES TO ALLEVIATE UNDIAGNOSED ENDOMETRIOSIS-RELATED PAIN. THE LONG-TERM GOAL OF THIS PROJECT IS TO DEVELOP A PUBLIC HEALTH MODEL THAT INCLUDES A VALIDATED QUESTIONNAIRE TO RAPIDLY IDENTIFY WOMEN IN EARLY RECOVERY FROM OPIATE USE DISORDERS AT RISK FOR ENDOMETRIOSIS, LINK THEM TO DIAGNOSIS AND TREATMENT, AND ULTIMATELY DECREASE ENDOMETRIOSIS-RELATED FATAL OVERDOSE. THE PROJECT WILL DEVELOP AN ENDOMETRIOSIS EDUCATION, SCREENING, BRIEF INTERVENTION, AND REFERRAL TO TREATMENT (ENDO-SBIRT) INTERVENTION THAT WILL BE DELIVERED INDIVIDUALLY TO WOMEN OVER 15-20 MINUTES. ENDO-SBIRT WILL INCLUDE: 1) A 10-MINUTE EDUCATIONAL MODULE ON ENDOMETRIOSIS, 2) SCREENING FOR ENDOMETRIOSIS RISK USING THE CHAPRON PATIENT QUESTIONNAIRE, 3) DISCUSSION OF THE RESULTS OF THE SCREENING DURING A BRIEF INTERVENTION, AND 4) REFERRAL OF WOMEN ASSESSED TO BE AT HIGH OR VERY HIGH RISK FOR ENDOMETRIOSIS TO A PROVIDER FOR FURTHER EVALUATION, DIAGNOSIS, AND TREATMENT. A TRAINED COMMUNITY HEALTH WORKER WILL DELIVER THE INTERVENTION UNDER THE SUPERVISION OF AN OB/GYN PHYSICIAN. A PARTNERSHIP WITH THREE SUBSTANCE USE TREATMENT PROVIDERS WILL ENGAGE 225 WOMEN IN THE INTERVENTION OVER THREE YEARS. THE GEOGRAPHIC CATCHMENT AREA WILL BE FIVE COUNTIES IN SOUTHERN NEW JERSEY WITH THE HIGHEST OVERDOSE RATES, INCLUDING ATLANTIC, CAMDEN, BURLINGTON, GLOUCESTER, AND OCEAN COUNTIES. THE POPULATION OF FOCUS WILL BE WOMEN OF REPRODUCTIVE AGE WHO USE ILLICIT OPIATES TO RELIEVE THE PAIN OF UNDIAGNOSED ENDOMETRIOSIS. OUTCOMES THAT WILL BE MEASURED INCLUDE THE NUMBER OF WOMEN ASSESSED TO BE AT HIGH RISK FOR ENDOMETRIOSIS, THE NUMBER OF WOMEN DIAGNOSED WITH AND TREATED FOR ENDOMETRIOSIS, AND CHANGES IN ENDOMETRIOSIS KNOWLEDGE AND QUALITY OF LIFE. THE GOALS ARE TO 1) IMPLEMENT AND EVALUATE THE ENDO-SBIRT INTERVENTION, 2) IDENTIFY AND TRACK EVIDENCE-BASED OUTCOMES, AND 3) TRANSITION ENDO-SBIRT TO SUSTAINABILITY. DISPARI

SOCIAL CAPITAL AND RESILIENCE OF OLDER PEOPLE EXPOSED TO HURRICANE SANDY

FUNCTIONAL ORGANIZATION OF LOCUS COERULEUS PROJECTIONS TO CNS MOTOR CIRCUITS -THIS PROJECT ADDRESSES MAJOR UNANSWERED QUESTIONS ABOUT THE STRUCTURE OF THE LOCUS COERULEUS-NOREPINEPHRINE (LC-NE) TRANSMITTER SYSTEM IN THE MAMMALIAN BRAIN AND ITS INFLUENCE ON CNS MOTOR CIRCUIT OPERATIONS AND MOVEMENT. THE LC-NE SYSTEM PROJECTS BROADLY THROUGHOUT THE CNS AND HAS BEEN SHOWN TO REGULATE COGNITION AND SENSORY SIGNAL PROCESSING ACROSS ALL STAGES OF WAKEFULNESS. ALTHOUGH IT PROMINENTLY INNERVATES MOTOR CENTERS OF THE BRAIN, A SIMILAR ROLE FOR THE SYSTEM IN REGULATING POSTURE, BALANCE, REFLEX AND GOAL-DIRECTED MOVEMENT ACROSS THE WAKING STATE IS LARGELY UNEXPLORED. WITH DEVELOPMENT OF NEW METHODOLOGY FOR VISUALIZATION OF LC NEURONS THAT SEND PROJECTIONS TO CNS MOTOR CIRCUITS, THE ANATOMY AND PHYSIOLOGY OF LC-NE INPUTS TO MOTOR CENTERS WILL BE DETERMINED. THESE EXPERIMENTS WILL PROVIDE INFORMATION THAT WILL ADVANCE OUR THINKING ABOUT HOW THE LC-NE SYSTEM EXERTS INFLUENCES ON NOT ONLY MOTOR ACTIVITY BUT ALSO SENSORY SIGNAL PROCESSING AND EXECUTIVE FUNCTION IN THE CONTEXT OF ADAPTIVE BEHAVIORS. MORE BROADLY THERE ARE BRAIN-WIDE APPLICATIONS FOR THIS APPROACH RELATIVE TO A HOST OF TRANSMITTER-SPECIFIC PATHWAYS IN THE CNS AND THE PROJECT ITSELF CAN BE USED AS A TRAINING PLATFORM FOR A NEXT GENERATION OF NEUROSCIENTISTS. THE PROJECT WILL ALSO FACILITATE TRAINING OF HIGH SCHOOL STUDENTS IN RESEARCH THROUGH THE HIGH SCHOOL BIOMEDICAL SCIENCE SCHOLAR PROGRAM AS WELL AS OF UNDERGRADUATES FROM UNDER-REPRESENTED GROUPS THROUGH THE RUTGERS-CAMDEN MARC PROGRAM. MORE SPECIFICALLY, THE CURRENT PROJECT FOCUSES ON THE INNERVATION OF CNS MOTOR CIRCUITS BY THE BRAINSTEM NUCLEUS LOCUS COERULEUS (LC) AND ITS IMPACT ON MOTOR NETWORK FUNCTION. FOR MORE THAN 50 YEARS LC WAS CONSIDERED HOMOGENEOUS IN STRUCTURE AND FUNCTION SUCH THAT ITS PRIMARY TRANSMITTER NOREPINEPHRINE (NE) COULD BE RELEASED UNIFORMLY AND ACT SIMULTANEOUSLY ON CELLS AND CIRCUITS THROUGHOUT THE BRAIN AND SPINAL CORD. HOWEVER, RECENT STUDIES FROM OUR LABORATORY AND ELSEWHERE HAVE PROVIDED COMPELLING EVIDENCE THAT LC IS MODULAR IN DESIGN, WITH SEGREGATED OUTPUT CHANNELS TO SENSORY, MOTOR, AND COGNITIVE CIRCUITRIES THROUGHOUT THE CNS AND THE POTENTIAL FOR DIFFERENTIAL RELEASE OF NE IN THESE FUNCTIONALLY DIVERSE PROJECTION FIELDS. THESE FINDINGS HAVE PROMPTED A RADICAL SHIFT IN THINKING ABOUT LC OPERATIONS AND DEMAND REVISION OF THEORETICAL CONSTRUCTS REGARDING THE IMPACT OF THE LC-NE SYSTEM ON BEHAVIORAL OUTCOMES INVOLVING NOT ONLY SENSORY AND COGNITIVE DOMAINS BUT ALSO MOVEMENT GENERATION. WITHIN THIS CONTEXT, A MAJOR GAP IN OUR KNOWLEDGE IS THE ANATOMICAL AND PHYSIOLOGICAL RELATIONSHIP BETWEEN THE LC-NE SYSTEM AND CNS MOTOR CONTROL CENTERS. THE INVESTIGATORS' APPROACH USING AN INTERSECTIONAL GENETIC MOUSE MODEL, RETROGRADE VIRAL VECTOR TRACING, AND EX VIVO ELECTROPHYSIOLOGY WILL ALLOW THEM TO DETERMINE IF LC-MOTOR CIRCUIT PROJECTION CELLS EXPRESS UNIQUE ELECTROPHYSIOLOGICAL PROPERTIES AND MAINTAIN AN ORGANIZED NETWORK OF DENDRITIC ARBORS AND AXON COLLATERALS THAT IS SPECIFIC TO MOTOR TERMINAL FIELDS AND CAPABLE OF SUPPORTING SELECTIVE, SYNCHRONOUS RELEASE OF NE IN CNS MOTOR CENTERS FOR THE PURPOSE OF COORDINATELY REGULATING OPERATIONS RESPONSIBLE FOR BALANCE, POSTURAL ADJUSTMENTS, AND EXECUTION OF VOLUNTARY MOVEMENTS. THIS AWARD REFLECTS NSF'S STATUTORY MISSION AND HAS BEEN DEEMED WORTHY OF SUPPORT THROUGH EVALUATION USING THE FOUNDATION'S INTELLECTUAL MERIT AND BROADER IMPACTS REVIEW CRITERIA.

PSYCHOSTIMULANT EFFECTS ON COGNITIVE FLEXIBILITY AND RISK-BASED DECISION-MAKING BEHAVIOR FOLLOWING REPETITIVE MILD TRAUMATIC BRAIN INJURY

INTEGRATED MENTAL HEALTH AWARENESS AND SUICIDE PREVENTION FROM OPIATES TRAINING FOR INDIVIDUALS AT THE FRONTLINE OF THE OPIOID EPIDEMIC IN NEW JERSEY - THE DEPARTMENT OF PSYCHIATRY AT ROWAN UNIVERSITY SCHOOL OF OSTEOPATHIC MEDICINE PROPOSES A SUICIDE PREVENTION FROM OPIATES TRAINING PROGRAM, CALLED SPOT THE SIGNS THAT WILL TRAIN 750 INDIVIDUALS FROM 10 COMMUNITY SECTORS THAT SERVE INDIVIDUALS WITH CO-OCCURRING OPIATE USE AND MENTAL HEALTH DISORDERS. THE TRAINING WILL HELP THEM TO RECOGNIZE MENTAL HEALTH DISORDERS THAT CAN LEAD TO SUICIDE BY INTENTIONAL OVERDOSE AND STEPS THEY CAN TAKE TO INTERVENE. ROWAN UNIVERSITY SCHOOL OF OSTEOPATHIC WILL DEVELOP AN INTEGRATED MENTAL HEALTH AWARENESS AND SUICIDE PREVENTION FROM OPIATES TRAINING PROGRAM FOR INDIVIDUALS WORKING AT THE FRONTLINE OF THE OPIOID EPIDEMIC IN NEW JERSEY. THE PROJECT WILL TARGET INDIVIDUALS FROM 10 COMMUNITY SECTORS THAT HAVE DIRECT CONTACT WITH INDIVIDUALS WHO USE OPIATES. THESE SECTORS INCLUDE SUBSTANCE USE TREATMENT PROVIDERS, INDIVIDUALS IN RECOVERY, FAMILIES WITH CHILDREN STRUGGLING WITH OPIATE USE DISORDERS, EMERGENCY MEDICINE PHYSICIANS, OFFICE-BASED OPIOID TREATMENT PROVIDERS, AND OTHERS. THE TRAINING WILL HELP PROVIDERS IDENTIFY THE SIGNS OF DEPRESSION AND OTHER SERIOUS MENTAL HEALTH AND EMOTIONAL DISTURBANCES THAT CAN LEAD TO SUICIDE BY INTENTIONAL OVERDOSE IN BOTH ADULTS AND YOUTH (AGES 14-17 YEARS OLD) WITH OPIATE USE AND MENTAL HEALTH DISORDERS. NEW JERSEY RANKS SIXTH IN THE NATION FOR THE NUMBER OF OVERDOSE DEATHS. THE GEOGRAPHIC CATCHMENT AREA INCLUDES FIVE COUNTIES WITH THE HIGHEST OVERDOSE FATALITIES IN THE STATE. THE PROJECT IS A RESPONSE TO A LOCAL AND NATIONWIDE SURGE IN OPIATE OVERDOSE DEATHS DURING THE COVID-19 PANDEMIC. IT IS ESTIMATED THAT OVER 25,000 (30%) OF THE 83,335 FATAL OVERDOSES DURING THE PANDEMIC MIGHT HAVE RESULTED FROM A SUICIDE ATTEMPT. PATIENTS WHO VISITED AN EMERGENCY DEPARTMENT AFTER AN OPIATE OVERDOSE WERE 18 TIMES MORE LIKELY TO DIE BY SUICIDE THAN THE GENERAL POPULATION IN THE YEAR AFTER THEIR VISIT. SEVERAL MENTAL HEALTH AWARENESS TRAINING PROGRAMS HAVE BEEN DEVELOPED FOCUSING ON PROVIDERS THAT SERVE YOUTH, VETERANS, AND OTHER HIGH-RISK POPULATIONS. HOWEVER, THERE ARE NO PROGRAMS TARGETING INDIVIDUALS AT THE FRONTLINE OF THE OPIOID EPIDEMIC. THIS PROJECT WILL DEVELOP A NEW SUICIDE PREVENTION FROM OPIATES TRAINING CURRICULUM CALLED SPOT THE SIGNS THAT USES THE EVIDENCE-BASED MENTAL HEALTH FIRST AID CONCEPT AND ADAPTED TO THIS HIGH-RISK POPULATION WITH CONTENT RECOMMENDED BY A NEW JERSEY SUICIDE PREVENTION FROM OPIATES COLLABORATIVE. THE GOALS ARE TO 1) ENHANCE THE TRAINING OF INDIVIDUALS IN DIRECT CONTACT WITH PERSONS WHO USE OPIATES TO RECOGNIZE THE SIGNS AND SYMPTOMS OF DEPRESSION AND OTHER SERIOUS MENTAL HEALTH/EMOTIONAL DISORDERS THAT CAN LEAD TO SUICIDE BY INTENTIONAL OVERDOSE, 2) INCREASE THE NUMBER OF INDIVIDUALS WITH CO-OCCURRING OPIATE USE DISORDERS AND MENTAL HEALTH SYMPTOMS WHO ARE REFERRED TO APPROPRIATE SERVICES TO PREVENT SUICIDE, 3) ENHANCE THE CAPACITY OF FIRST RESPONDERS TO RECOGNIZE MENTAL HEALTH CRISES IN INDIVIDUALS WITH OPIATE USE DISORDERS AND TO EMPLOY CRISIS DE-ESCALATION TECHNIQUES LEADING TO THEIR ENGAGEMENT IN CARE, AND 4) INCREASE ACCESS TO WRITTEN AND ELECTRONIC MENTAL HEALTH TREATMENT RESOURCES FOR PERSONS WHO USE OPIATES AT RISK FOR SUICIDE BY INTENTIONAL OVERDOSE. EVALUATION OF THE PROGRAM WILL BE CONDUCTED BY RUTGERS UNIVERSITY.

INHIBITION OF HEDGEHOG SIGNALING AS A THERAPEUTIC STRATEGY FOR OSCC - ABSTRACT ORAL CANCER IS A GLOBAL HEALTH CRISIS. WITHIN THE UNITED STATES ALONE, AN ESTIMATED 58,000 ORAL CANCER CASES AND OVER 12,000 RELATED DEATHS ARE PROJECTED FOR 2024. MORE THAN 90% OF ORAL CANCERS ARE ORAL SQUAMOUS CELL CARCINOMA (OSCC). CURRENT TREATMENTS HAVE CHALLENGES, AND MORE EFFECTIVE TREATMENTS ARE REQUIRED. GROWING EVIDENCE SUGGESTS HEDGEHOG (HH) SIGNALING AS ONE OF THE KEY DRIVERS OF OSCC PROGRESSION AND METASTASIS. HH SIGNALING INDUCES THE EXPRESSION OF GENES THAT PROMOTE CANCER, INCLUDING THOSE INVOLVED IN CELL PROLIFERATION AND MIGRATION. HH PATHWAY INHIBITION (HPI) DRUGS HAVE BEEN FDA-APPROVED OR UNDER CLINICAL TRIAL FOR TREATING VARIOUS CANCERS. HOWEVER, THE THERAPEUTIC POTENTIAL OF HPI DRUGS AGAINST OSCC IS NOT CLEARLY ELUCIDATED. WE HYPOTHESIZE HPI COULD SERVE AS A THERAPEUTIC STRATEGY FOR OSCC. TO TEST THIS HYPOTHESIS, WE EVALUATED THE EFFECTS OF THE HPI DRUG SONIDEGIB ON THE VIABILITY OF OSCC CELLS, WHICH WERE RECENTLY DERIVED FROM OSCC CANCER PATIENT TISSUE AND ADAPTED TO IN VITRO CULTURES. OUR INNOVATIVE RESEARCH DESIGN INCLUDES OSCC CELLS REPRESENTING CURRENT DISEASE CHARACTERISTICS. OUR PRELIMINARY DATA SHOW THAT SONIDEGIB SIGNIFICANTLY REDUCES OSCC CELL VIABILITY. THUS, OUR FIRST AIM INVESTIGATES HOW SONIDEGIB DECREASES OSCC CELL VIABILITY. WE WILL EMPLOY IMAGING AND VIABILITY ASSAYS, AS WELL AS GENE AND PROTEIN ANALYSES, TO EVALUATE THE EFFECTS OF SONIDEGIB ON OSCC CELLS. WE WILL SPECIFICALLY ASSESS REDUCTIONS IN CELL PROLIFERATION, INFLAMMATION, OXIDATIVE STRESS, AND APOPTOSIS INHIBITION. ADDITIONALLY, SONIDEGIB CAN REDUCE THE EXPRESSION OF MOTILITY MARKERS, INCLUDING PODOPLANIN, MMP-2, AND MMP-9. WE HYPOTHESIZE THAT SONIDEGIB CAN INHIBIT OSCC CELL MIGRATION. IN OUR SECOND AIM, WE WILL UTILIZE MOTILITY ASSAYS TO DETERMINE HOW SONIDEGIB DECREASES OSCC CELL MIGRATION. OUR THIRD AIM FOCUSES ON DETERMINING THE SYNERGISTIC EFFECTS OF PAIRING SONIDEGIB WITH FDA-APPROVED DRUGS CURRENTLY IN TRIALS FOR OSCC BUT NOT TARGETING HH SIGNALING. WE HYPOTHESIZE THAT THESE COMBINATION THERAPIES WILL EXERT A GREATER INHIBITORY EFFECT ON OSCC GROWTH AND MOTILITY COMPARED TO INDIVIDUAL HPI TREATMENT. WE WILL EVALUATE CYTOTOXIC, CYTOSTATIC AND MIGRATORY MARKERS FROM AIM 1 AND AIM 2 TO QUANTIFY THE SYNERGISTIC EFFECTS. WE WILL EMPLOY RNA-SEQ TO IDENTIFY KEY DRIVER GENES TO FURTHER UNDERSTAND THE MECHANISM OF ACTION OF SONIDEGIB ON OSCC CELLS, BOTH ALONE AND IN COMBINATION WITH OTHER DRUGS. THE IMPLICATIONS OF OUR RESEARCH ARE SIGNIFICANT: BY TARGETING A MAJOR PATHWAY INVOLVED IN OSCC, WE AIM TO IMPROVE TREATMENT OUTCOMES AND THE QUALITY OF LIFE FOR OSCC PATIENTS. WE FOCUS ON ORALLY AVAILABLE DRUGS TO OFFER CONVENIENT ADMINISTRATION AND PHARMACOKINETICS TO OFFER EXPEDITE RELIEF TO THOSE SUFFERING FROM OSCC. TOGETHER, OUR WORK WILL ALLEVIATE THE BURDEN OF THIS DEVASTATING DISEASE AND PAVE THE WAY FOR MORE EFFECTIVE OSCC TREATMENTS. ADDITIONALLY, THE PROPOSAL WILL SERVE AS AN EXCELLENT PLATFORM FOR TRAINING UNDERGRADUATE AND GRADUATE STUDENTS AT ROWAN UNIVERSITY IN ORAL CANCER RESEARCH AND CANCER DRUG DISCOVERY DEVELOPMENT UTILIZING VARIOUS MOLECULAR BIOLOGY TECHNIQUES AND BIOINFORMATICS.

PROVIDER'S CLINICAL SUPPORT SYSTEM - UNIVERSITIES - ROWAN-VIRTUA SCHOOL OF OSTEOPATHIC MEDICINE (RVSOM) PROPOSES THE CREATION AND IMPLEMENTATION OF A COMPREHENSIVE OSTEOPATHIC PROVIDER CLINICAL SUPPORT SYSTEM CURRICULUM THAT WILL TRAIN STUDENTS ABOUT SUBSTANCE USE DISORDER TREATMENT EARLY IN THEIR MEDICAL SCHOOL CAREER THROUGH BOTH DIDACTIC AND CLINICAL TRAINING OPPORTUNITIES. THE ULTIMATE GOAL OF THIS PROJECT WILL BE TO REDUCE STIGMA AND INCREASE ACCESS TO SUD SCREENINGS, ASSESSMENTS, AND SERVICES WITHIN THE SOUTHERN NEW JERSEY REGION, WITH A PARTICULAR EMPHASIS ON PRESCRIBING MEDICATIONS FOR ALCOHOL AND OPIATE USE DISORDERS. THE POPULATION OF FOCUS FOR THIS PROJECT WILL BE MEDICAL STUDENTS AT RVSOM. THE GEOGRAPHIC CATCHMENT AREA OF THIS PROJECT WILL INCLUDE THE TWO MEDICAL SCHOOL CAMPUSES OF RVSOM, LOCATED IN THE CAMDEN AND GLOUCESTER COUNTIES OF NEW JERSEY. IN ADDITION TO PROVIDING DIDACTIC TRAINING AT RVSOM LOCATIONS, OUR PROJECT WILL PROVIDE TRAINING PLACEMENTS AND REACH REGIONAL HEALTH SYSTEM LOCATIONS IN ATLANTIC, CAMDEN, CUMBERLAND, GLOUCESTER, AND SALEM COUNTIES. THERE IS A SIGNIFICANT NEED FOR FUTURE PHYSICIANS IN OUR REGION TO BE EDUCATED ON BEST PRACTICES FOR SUD TREATMENT, STIGMA REDUCTION, AND EQUITABLE ACCESS TO SUD CARE. THE FOUR SOUTH JERSEY COUNTIES IN OUR GEOGRAPHIC CATCHMENT AREA FOR THIS PROJECT HAVE AMONG THE HIGHEST RATES OF DRUG OVERDOSE DEATHS IN THE STATE, WITH ATLANTIC, SALEM, CAMDEN, CUMBERLAND, AND GLOUCESTER COUNTIES RANKED #1, #3, #4, #5, AND #6 FOR DRUG OVERDOSE DEATHS PER 100,000 IN NEW JERSEY.1 OVER 85% OF RVSOM STUDENTS ARE RESIDENTS OF NEW JERSEY AND OVER 50% PRACTICE IN THE STATE AFTER GRADUATION. OVER 80% OF THESE STUDENTS GO ON TO PRACTICE IN EITHER FAMILY MEDICINE OR ANOTHER PRIMARY CARE SPECIALTY. AS A RESULT, THERE IS A HIGH PROBABILITY THAT RVSOM STUDENTS WILL ENCOUNTER A PATIENT WITH A SUBSTANCE USE PROBLEM AS PART OF THEIR PRIMARY CARE PRACTICE AFTER GRADUATION. INCREASING THE NUMBER OF OSTEOPATHIC PHYSICIANS TRAINED BY RVSOM TO IDENTIFY AND TREAT PATIENTS WITH SUBSTANCE USE DISORDERS DURING PRIMARY CARE VISITS USING THE PROPOSED CURRICULUM WILL HELP TO ADDRESS THE TREATMENT GAP IN NEW JERSEY AND IMPROVE OUTCOMES THROUGHOUT OUR REGION. THE GOALS OF THIS PROJECT ARE TO 1) DEVELOP AN OSTEOPATHIC PROVIDER CLINICAL SUPPORT SYSTEM CURRICULUM TO INTRODUCE OSTEOPATHIC MEDICAL STUDENTS AND OTHER HEALTH PROFESSIONS STUDENTS TO SUD TREATMENT PRINCIPLES USING A LONG-TERM ILLNESS AND RECOVERY MANAGEMENT MODEL, 2) CREATE OSTEOPATHIC PROVIDER CLINICAL SUPPORT SYSTEM CURRICULAR MATERIALS AND DEVELOP STRATEGIES TO INCREASE REACH AND ACCESSIBILITY, AND 3) IMPLEMENT THE OSTEOPATHIC PROVIDER CLINICAL SUPPORT SYSTEM CURRICULUM AMONG ALL MEDICAL STUDENTS AT ROWAN-VIRTUA SCHOOL OF OSTEOPATHIC MEDICINE, AND EXPAND THE REACH OF THE PROGRAM BY MAKING MATERIALS AVAILABLE TO ADDITIONAL OSTEOPATHIC MEDICAL SCHOOLS, OTHER HEALTH PROFESSIONS STUDENTS, AND PROFESSIONALS IN REGIONAL HEALTHCARE SYSTEMS. THE OSTEOPATHIC PROVIDER CLINICAL SUPPORT SYSTEM CURRICULUM DEVELOPED BY OUR PROGRAM WILL CONSIST OF FOUR TWO-HOUR WEBINARS TO BE COMPLETED BY ALL THIRD-YEAR MEDICAL STUDENTS AT RVSOM. EACH WEBINAR WILL INCLUDE PRE- AND POST-TEST ASSESSMENTS TO DETERMINE THE EFFECTIVENESS OF THE CURRICULUM. 1 UNIVERSITY OF WISCONSIN POPULATION HEALTH INSTITUTE. COUNTY HEALTH RANKINGS & ROADMAPS 2024. WWW.COUNTYHEALTHRANKINGS.ORG.

NORTHEAST COLLABORATIVE TO IMPROVE ACCESS TO OVERDOSE TREATMENT - THE NEUROMUSCULOSKELETAL INSTITUTE AT ROWAN UNIVERSITY SCHOOL OF OSTEOPATHIC MEDICINE, THE WEST VIRGINIA SCHOOL OF OSTEOPATHIC MEDICINE, AND THE PHILADELPHIA COLLEGE OF OSTEOPATHIC MEDICINE WILL TRAIN MEDICAL STUDENTS TO TRAIN 35 PHYSICIANS IN 7 HEALTHCARE SETTINGS IN COMMUNITIES WITH HIGH OVERDOSE RATES IN NEW JERSEY, WEST VIRGINIA, AND PHILADELPHIA WHO WILL TRAIN 1,250 ADDITIONAL HEALTHCARE PROVIDERS WHO WILL THEN TRAIN 400 PATIENTS ON NALOXONE. THE GEOGRAPHIC CATCHMENT AREA WILL BE 5 COUNTIES WITH THE HIGHEST OVERDOSE RATES IN NEW JERSEY, PENNSYLVANIA, AND WEST VIRGINIA. IN NEW JERSEY, THE PROJECT WILL FOCUS ON TRAINING HEALTHCARE PROVIDERS IN THE CITY OF CAMDEN, LOCATED IN CAMDEN COUNTY, WHICH HAD THE SECOND-HIGHEST NUMBER OF FATAL OVERDOSES IN 2022. THE PROJECT WILL ALSO TARGET HEALTHCARE PROVIDERS IN THE KENSINGTON AND ALLEGHENY SECTION OF PHILADELPHIA, PENNSYLVANIA, THE LARGEST OPEN-AIR DRUG MARKET ON THE EAST COAST. THREE COUNTIES IN WEST VIRGINIA, WHICH HAD THE HIGHEST CONFIRMED OVERDOSE DEATH RATE IN THE NATION (81.4 PER 100,000) IN 2020, WILL ALSO BE TARGETED, INCLUDING RURAL CABELL, WAYNE, AND LINCOLN COUNTIES LOCATED IN THE SOUTHWEST REGION OF THE STATE. IN 2021, CABELL COUNTY HAD AN OVERDOSE MORTALITY RATE OF 130.60 PER 100,000 RESIDENTS, OVER FOUR TIMES THE US RATE OF 32.4. THE POPULATION OF FOCUS WILL BE OSTEOPATHIC MEDICAL STUDENTS, PHYSICIANS, AND PHARMACISTS WORKING IN SEVEN HEALTHCARE SETTINGS IN THESE HIGH OVERDOSE AREAS. THE GOALS ARE 1) EXPAND ACCESS TO OVERDOSE TREATMENT BY IMPLEMENTING A TRAIN-THE-TRAINER MODEL WITH PRESCRIBERS OF NALOXONE IN HIGH OVERDOSE COMMUNITIES IN NEW JERSEY, PENNSYLVANIA, AND WEST VIRGINIA TO BUILD COMPETENCY IN CO-PRESCRIBING NALOXONE TO PERSONS TAKING OPIATES. 2) DECREASE OVERDOSE IN COMMUNITIES IN THE FIVE-COUNTY REGION BY CO-PRESCRIBING NALOXONE AND TRAINING PATIENTS ON HOW TO TRAIN A PEER ON HOW TO ADMINISTER NALOXONE. 3) CONTINUALLY EXPAND ACCESS TO OVERDOSE TREATMENT THROUGH SUSTAINABILITY PLANNING, ADDING ADDITIONAL HEALTHCARE SYSTEMS TO THE PROJECT, AND CREATING RESOURCES FOR NATIONAL REPLICATION. ROWAN UNIVERSITY SCHOOL OF OSTEOPATHIC MEDICINE, WHICH OPERATES THE SOUTHERN NEW JERSEY MAT CENTER OF EXCELLENCE, WILL PARTNER WITH THE WEST VIRGINIA SCHOOL OF OSTEOPATHIC MEDICINE AND THE PHILADELPHIA COLLEGE OF OSTEOPATHIC MEDICINE TO FORM A NORTHEAST COLLABORATIVE TO IMPROVE ACCESS TO OVERDOSE TREATMENT. THE COLLABORATIVE WILL TRAIN HEALTHCARE PROFESSIONALS IN THE FIVE COUNTIES OF NEW JERSEY, WEST VIRGINIA, AND PENNSYLVANIA WITH THE HIGHEST OVERDOSE RATES IN THE NATION. THE PROJECT WILL USE A TRAIN-THE-TRAINER APPROACH AND A DIVERSE GROUP OF OSTEOPATHIC MEDICAL STUDENTS IN EACH OF THE THREE SCHOOLS TO FORM AN OSTEOPATHIC TASKFORCE FOR THE TREATMENT OF OVERDOSE CALLED PROJECT OTTO. STUDENTS WILL IDENTIFY 35 PHYSICIAN CHAMPIONS IN HEALTH CARE SETTINGS WHERE THEY ROTATE IN THE FIVE-COUNTY AREA DURING THEIR CLINICAL YEARS AND TRAIN THEM ON A NALOXONE TRAINING CURRICULUM CALLED M5PM. THE STUDENTS WILL THEN ASK THE PHYSICIAN CHAMPIONS TO TRAIN 1,250 ADDITIONAL PHYSICIANS AND PHARMACISTS. THOSE PHYSICIANS WILL, IN TURN, TRAIN 400 PATIENTS TAKING OPIATES AND CO-PRESCRIBED NALOXONE USING M5PM. THE EVALUATION WILL BE PERFORMED BY STAFF IN THE OFFICE OF THE DEAN FOR RESEARCH AT ROWANSOM.

CAPE CTC COVIS-19 RELIEF FUNDS

HEALTH CAREERS OPPORTUNITY PROGRAM

MENTAL ILLNESS AND CO-OCCURRING MENTAL ILLNESS AND SUBSTANCE ABUSE CRISIS CO-RESPONDERS AND SPECIALIZED POLICE RESPONSES.

IMPACT AID PROGRAM, TITLE VII, SECTION 7003

UNKNOWN TITLE

FORMULA GRANTS TO LEAS

THE PURPOSE OF THE COPS OFFICE SCHOOL VIOLENCE PREVENTION PROGRAM (SVPP) IS TO IMPROVE SECURITY AT SCHOOLS AND ON SCHOOL GROUNDS THROUGH SCHOOL SAFETY PROGRAMS AND TECHNOLOGY. FUNDING UNDER THIS AWARD PROGRAM WILL BE USED BY THE SCHOOL JURISDICTION TO IMPLEMENT EVIDENCE-BASED PROGRAMS TO IMPROVE SCHOOL SECURITY AND PROMOTE A POSITIVE LEARNING ENVIRONMENT FOR ALL STUDENTS.

DISTANCE LEARNING GRANT

INVESTIGATING THE POTENTIAL IMPACT OF A LOW-FAT HIGH FIBER DIET IN LIMITING TBI-INDUCED NEURODEGENERATIVE AND INFLAMMATORY CHANGES. - PROJECT SUMMARY: THE SEQUELAE OF TRAUMATIC BRAIN INJURY (TBI) PATHOLOGY COMPRISES PRIMARY INJURY WHERE IMPACTING FORCE(ES) INDUCE PHYSICAL DAMAGE, INCLUDING THE BREAKDOWN OF THE BLOOD-BRAIN BARRIER (BBB). THUS THE PRIMARY INJURY LAUNCHES SECONDARY INJURY COMPRISING CHRONIC INFLAMMATION THAT INCLUDES MIGRATION OF LEUKOCYTES, GLIOSIS, AND THE RELEASE OF NUMEROUS INFLAMMATORY MEDIATORS. THUS, SECONDARY INJURY EXACERBATES THE DAMAGE INCURRED DURING THE PRIMARY INJURY AND IS CONSIDERED MORE DEVASTATING. THOUGH VARIOUS DRUGS ARE TESTED TO CURTAIL SECONDARY INJURY, THESE TRIALS HAVE FAILED, AND THE PROSPECT OF IDENTIFYING A TBI DRUG LOOKS BLEAK. HENCE, THE TREATMENT OPTIONS FOR TBI SURVIVORS ARE RESTRICTED TO PALLIATIVE CARE. RECENTLY, THERE HAVE BEEN SEVERAL PUBLICATIONS EMPHASIZING THE NEUROPROTECTIVE AND ANTI-INFLAMMATORY EFFECTS OF VARIOUS DIETS. ADDITIONALLY, SINCE CARDIOVASCULAR DISEASES (CVDS) ARE ONE OF THE MOST IMPORTANT COMORBID CHANGES IN TBI SURVIVORS, STUDIES EMPHASIZING THE ROLE OF CVD IN TBI PATHOLOGY ARE NON-EXISTENT. OUR PROPOSED RESEARCH AIMS TO COMPARE THE BENEFICIAL EFFECTS OF A LOW-FAT, HIGH-FIBER DIET (LFHFD) IN REDUCING TBI-ASSOCIATED BBB BREAKDOWN, NEUROINFLAMMATION, AND IMMUNE RESPONSE OVER THE STANDARD WESTERN DIET (WD) THAT IS HIGH-FAT AND CHOLESTEROL, AND LOW IN FIBER IN A MOUSE MODEL WITH CHRONIC CVD AND TBI. WE ARE EMPLOYING AMERICAN HEART ASSOCIATION (AHA) RECOMMENDED MOUSE MODEL (LDL RECEPTOR-DEFICIENT MOUSE MODEL (LDLR-KO)) FOR THIS STUDY. WE HYPOTHESIZE THAT THE UPTAKE OF WD FURTHER EXACERBATES THE COMBINED EFFECTS OF CVD AND TBI AND WILL CAUSE CHRONIC BBB DYSFUNCTION, PREDISPOSING INDIVIDUALS TO CHRONIC INFLAMMATORY CHANGES. TO TEST THIS HYPOTHESIS, WE WILL MAINTAIN LDLR-KO MICE EITHER ON WD OR LFHFD FROM THE SECOND MONTH TO THE SIXTH MONTH. IN THE FIRST WEEK OF THE SEVENTH MONTH, HALF OF THE LDLR-KO MICE MAINTAINED ON A WD OR LFHFD DIET WILL UNDERGO TBI (CONTROLLED CORTICAL IMPACT, CCI) OR SHAM INJURY (CRANIOTOMY WITHOUT CCI). ALL ANIMALS ON THEIR RESPECTIVE DIETS WILL BE MAINTAINED FOR THREE TO SIX MONTHS. ANIMALS WILL BE EUTHANIZED AT THE END OF THE 9TH AND 12TH MONTH, AND BRAIN SAMPLES WILL BE EXTRACTED AND PROCESSED FOR ROUTINE PARAFFIN-EMBEDDED TISSUE PROCESSING AND BRIGHT FIELD IMMUNOHISTOCHEMISTRY FOR INVESTING BBB FUNCTION (AIM 1). WE WILL ALSO STUDY THE IMPACT OF DIETS ON THE EXPRESSION OF VARIOUS NEUROINFLAMMATORY MARKERS AND OVERALL IMMUNE RESPONSES (AIM 2). A SECOND SET OF ANIMALS WILL BE PREPARED, AND THEIR BRAIN SAMPLES WILL BE USED FOR PROTEIN AND GENE EXPRESSION STUDIES OF VARIOUS NEUROINFLAMMATION MARKERS BY EMPLOYING WESTERN BLOT AND REAL-TIME POLYMERASE CHAIN REACTION. WE WILL ALSO COLLECT PRE-INJURY AND POST-INJURY BLOOD SAMPLES FROM THESE MICE AND RUN A LUMINEX ASSAY TO MONITOR SERUM LEVELS OF VARIOUS IMMUNE MEDIATORS IN BLOOD SAMPLES. WE EXPECT WD TO EXACERBATE BBB DYSFUNCTION AND INFLAMMATORY SEQUELAE COMPARED TO LFHFD. UPON COMPLETING THIS STUDY, WE WILL HAVE PRELIMINARY DATA EMPHASIZING THE BENEFICIAL EFFECTS OF LFHFD OVER WD IN REDUCING BBB PERMEABILITY, NEUROINFLAMMATION, AND IMMUNE RESPONSES IN LONG-TERM TBI SURVIVORS.

FORMULA GRANTS TO LEAS

FORMULA GRANTS TO LEAS

MITOCHONDRIAL TRANSCRIPTION AND ITS REGULATION IN NEURAL TISSUE

UNKNOWN TITLE

FORMULA GRANTS TO LEAS

INDIAN EDUCATION FORMULA GRANTS TO LEAS

INDIAN EDUCATION FORMULA GRANTS TO LEAS

TRANSLATIONAL CONTROL OF MITOCHONDRIAL GENE EXPRESSION

CADHERINS, CONTACT NORMALIZATION, AND TARGETING PODOPLANIN TO TREAT ORAL CANCER - SUMMARY ORAL CANCER KILLS OVER 8,000 PEOPLE IN THE USA AND 120,000 PEOPLE WORLDWIDE EVERY YEAR. IN ADDITION TO THIS MORTALITY, ORAL CANCER SURVIVORS SUFFER FROM PERMANENT SEQUELAE AND DECREASED QUALITY OF LIFE. OVER 90% OF ORAL CANCERS ARE CAUSED BY ORAL SQUAMOUS CELL CARCINOMA (OSCC). THIS PROJECT IS BASED ON THE PREMISE THAT NONTRANSFORMED CELLS CAN CONTROL THE GROWTH OF NEIGHBORING TRANSFORMED CELLS BY CONTACT NORMALIZATION. DIRECT CONTACT BETWEEN TRANSFORMED AND NONTRANSFORMED CELLS IS REQUIRED FOR THIS PROCESS. HOWEVER, JUNCTIONS RESPONSIBLE FOR CONTACT NORMALIZATION, AND GENE PRODUCTS THAT ENABLE CANCER CELLS TO OVERCOME THIS FORM OF GROWTH CONTROL, HAVE NOT BEEN DEFINED. WE FOUND THAT CONTACT NORMALIZATION SUPPRESSES EXPRESSION OF THE TRANSMEMBRANE MUCIN RECEPTOR PODOPLANIN (PDPN) DOWNSTREAM OF ONCOGENIC SRC TYROSINE KINASE ACTIVITY. MANY CANCER CELLS, INCLUDING OSCCS, EXPRESS PDPN TO PROMOTE CANCER PROGRESSION. PRECANCEROUS ORAL LESIONS (E.G. LEUKOPLAKIA) THAT EXPRESS HIGH LEVELS OF PDPN ARE SEVERAL TIMES MORE LIKELY TO BECOME CANCERS THAN LESIONS THAT DO NOT EXPRESS PDPN. IN ADDITION, PDPN HAS BEEN IDENTIFIED AS AN IMMUNE CELL CHECKPOINT COREPRESSOR. INDEED, PDPN HAS EMERGED AS A FUNCTIONALLY RELEVANT BIOMARKER AND CHEMOTHERAPEUTIC TARGET THAT CAN BE USED TO DETECT AND TREAT ORAL CANCER. OUR RATIONALE IS BASED ON THE FACT THAT CADHERIN JUNCTIONS ARE OFTEN DISRUPTED BETWEEN TRANSFORMED CELLS, WHILE PDPN EXPRESSION IS ENHANCED. PDPN ANTIBODIES AND MAACKIA AMURENSIS SEED LECTIN (MASL) EFFICIENTLY TARGET PDPN TO DECREASE OSCC CELL MOTILITY AND VIABILITY. MOREOVER, MASL IS NONTOXIC AND CAN BE ADMINISTERED ORALLY TO INHIBIT TUMOR GROWTH AND VASCULARIZATION WITHOUT NOTABLE SIDE EFFECTS IN PRECLINICAL STUDIES. WE HYPOTHESIZE THAT CADHERINS MEDIATE CONTACT NORMALIZATION TO SUPPRESS PDPN EXPRESSION, AND PDPN CAN BE UTILIZED AS A FUNCTIONALLY RELEVANT CHEMOTHERAPEUTIC TARGET TO SYNERGISTICALLY INHIBIT TUMOR CELL GROWTH AND AUGMENT ANTICANCER IMMUNE RESPONSE. WE PROPOSE 3 SPECIFIC AIMS TO TEST THIS HYPOTHESIS. IN AIM 1, WE WILL DETERMINE IF CADHERINS ARE NEEDED FOR NONTRANSFORMED CELLS TO NORMALIZE MORPHOLOGY, GROWTH, MOTILITY, WNT SIGNALING, AND PDPN EXPRESSION OF ADJACENT SRC TRANSFORMED AND OSCC CELLS. IN AIM 2, WE WILL DETERMINE IF PDPN SIGNALING ENABLES SRC TRANSFORMED FIBROBLASTS AND OSCC CELLS TO OVERRIDE GROWTH CONTROL BY NEIGHBORING NONTRANSFORMED CELLS. IN AIM 3, WE WILL DETERMINE IF MASL CAN TARGET PDPN TO AUGMENT HOST IMMUNE RESPONSE IN CONCERT WITH ITS ABILITY TO INHIBIT OSCC CELL GROWTH IN AN FDA APPROVED PHASE 1 HUMAN CLINICAL TRIAL ON ORAL CANCER PATIENTS. THIS PROJECT WILL EXPOSE GRADUATE AND MEDICAL STUDENTS TO BASIC RESEARCH IN MOLECULAR AND CELL BIOLOGY TO ELUCIDATE FUNDAMENTAL MECHANISMS BEHIND CONTACT NORMALIZATION. THIS WORK WILL ENHANCE OUR ABILITIES TO DETECT, PREVENT, AND TREAT ORAL CANCER, AS WELL AS OTHER CANCERS THAT ARE DRIVEN BY PDPN SIGNALING.

TARGETING PODOPLANIN TO PREVENT AND COMBAT ORAL CANCER

STEM CELL-BASED THERAPY FOR CANAVAN DISEASE

DEFINING GENE EXPRESSION AND REGULATION IN LINGUAL TASTE AND NON-TASTE PAPILLA EPITHELIUM

UNKNOWN TITLE

INDIAN EDUCATION FORMULA GRANTS TO LEAS

INDIAN EDUCATION FORMULA GRANTS TO LEAS

STRESS-INDUCED LOCUS COERULEUS DYSFUNCTION AS A MEDIATOR OF OPIOID ABUSE - PROJECT SUMMARY OPIOID ABUSE AND ANXIETY DISORDERS ARE AMONGST THE MOST PREVALENT PSYCHIATRIC CONDITIONS IN THE UNITED STATES, AND DIAGNOSIS OF ONE INCREASES THE RISK OF DEVELOPING THE OTHER. GIVEN THEIR OVERLAP AND THE CURRENT STATE OF THE OPIOID EPIDEMIC GRIPPING THE COUNTRY, IT IS OF CRITICAL IMPORTANCE TO CLARIFY THE MECHANISMS THAT LINK THESE TWO CONDITIONS. THE LOCUS COERULEUS (LC) IS A BRAINSTEM NUCLEUS INVOLVED IN A WIDE ARRAY OF CENTRAL NERVOUS SYSTEM FUNCTIONS. STRESS ACTIVATES LC AND PROMOTES HYPERVIGILANT ANXIETY-LIKE BEHAVIOR. ALTHOUGH MANY STUDIES IN THE PAST HAVE INVESTIGATED HOW STRESS AFFECTS THE FUNCTION OF THE LC AT SHORT INTERVALS, LESS IS KNOWN ABOUT HOW STRESSOR EXPOSURE CAUSES LONG TERM CHANGES IN THE NUCLEUS THAT ARE ASSOCIATED WITH A CHRONICALLY ALTERED BEHAVIORAL STATE. RECENT OBSERVATIONS FROM OUR LABORATORY SHOW THAT AN ACUTE TRAUMATIC STRESSOR CAN PRODUCE LONG-LASTING ELEVATIONS IN ANXIETY-LIKE BEHAVIOR AND LC ACTIVITY, AND FURTHERMORE, THESE EFFECTS MAY BE RELATED TO ALTERED FUNCTION OF LC OPIOID RECEPTORS. THIS IS OF GREAT IMPORTANCE DUE TO THE FACT THAT ENDOGENOUS OPIOID SIGNALING IN LC HELPS TERMINATE THE STRESS RESPONSE, REDUCE LC ACTIVITY, AND PROMOTE A RETURN TO A NON-ANXIOUS BEHAVIORAL STATE. THUS, IF STRESS BLUNTS THE FUNCTION OF OPIOID RECEPTORS IN LC, ENDOGENOUS OPIOIDS MAY NOT BE SUFFICIENT TO LIMIT LC DISCHARGE TO LEVELS THAT DO NOT PROMOTE ANXIETY. IN THIS SCENARIO, INDIVIDUALS WITH A HISTORY OF CHRONIC OR TRAUMATIC STRESS MIGHT BE PREDISPOSED TO USE ABUSED OPIOIDS, BECAUSE OF THEIR ABILITY TO POTENTLY INHIBIT LC. FURTHERMORE, INDIVIDUALS WHO HAVE RECOVERED FROM OPIOID DEPENDENCE MIGHT BE LIKELY TO RELAPSE WHEN FACED WITH A NEW STRESSOR. THEREFORE, UNDERSTANDING THE IMPACT OF STRESSOR EXPOSURE ON LC FUNCTION AND OPIOID SIGNALING, AND THE ROLE OF ENDOGENOUS OPIOID SIGNALING IN MOTIVATED BEHAVIOR, MAY PROVIDE INSIGHTS TOWARDS THERAPEUTIC APPROACHES TO COUNTERACT SOME OF THE ABNORMAL BEHAVIORS SEEN IN COMORBID ANXIETY AND OPIOID USE DISORDERS. THE GOALS OF THIS PROJECT ARE FIRST TO SHOW THAT ENHANCING OPIOID SIGNALING IN LC IS ANXIOLYTIC AND REINFORCING IN ANIMALS THAT HAVE EXPERIENCED STRESS BECAUSE OF HOW IT REDUCES LC HYPERACTIVITY, AND THEREFORE NEGATIVE EMOTION. SECOND, WE AIM TO SHOW THAT TRAUMATIC STRESS ALSO MAKES ANIMALS MORE LIKELY TO SELF- ADMINISTER ABUSED OPIOIDS BECAUSE OF STRESS-INDUCED CHANGES IN LC. THE HYPOTHESIS IS THAT STRESS INCREASES LC ACTIVITY, WHICH CORRELATES WITH ANXIETY, AND REDUCES THE ABILITY OF LC TO BE INHIBITED BY ENDOGENOUS OPIOIDS. THEREFORE, ANIMALS WILL ENGAGE IN BEHAVIORS THAT LEAD TO ARTIFICIALLY ENHANCED ENDOGENOUS OPIOID SIGNALING OR DELIVERY OF INTRAVENOUSLY ABUSED OPIOIDS, BECAUSE OF THEIR ABILITY TO REDUCE LC HYPERACTIVITY. THE RESULTS OF THESE EXPERIMENTS WILL DEMONSTRATE THAT A STRESS-INDUCED NEUROADAPTATION OUTSIDE OF THE CLASSICAL ADDICTION CIRCUITRY HAS THE ABILITY MAKE ANIMALS MORE LIKELY TO SELF-ADMINISTER ABUSED OPIOIDS. SUCH FINDINGS WOULD HAVE IMPORTANT IMPLICATIONS FOR MECHANISMS OF AND TREATMENTS FOR BOTH ANXIETY AND OPIOID USE DISORDERS.

FORMULA GRANTS TO LEAS

PHYSIOLOGICAL GENOMICS AND SEX DIFFERENCES OF CENTRAL VASOPRESSIN AND SEROTONIN CIRCUITS

FORMULA GRANTS TO LEAS

CARDIOVASCULAR RISK FACTORS AND PRETERM DELIVERY

THE ROLE OF CYCLIN C IN MEDIATING NEURODEGENERATIVE PROTEINOPATHIES - ALZHEIMER’S DISEASE (AD), PARKINSON’S DISEASE (PD), LEWY BODY DISEASE, AMYOTROPHIC LATERAL SCLEROSIS (ALS), FRONTOTEMPORAL LOBAR DEGENERATION (FTLD), AND PRION DISEASE CONSTITUTE A GROUP OF NEUROGENERATIVE DISEASES CALLED PROTEINOPATHIES. THEY ARE CHARACTERIZED BY THE ACCUMULATION OF AGGREGATED AND MISFOLDED PROTEINS IN THE CENTRAL NERVOUS SYSTEM. THE IDENTITIES OF THE AGGREGATED DISEASE PROTEINS HAVE BEEN IDENTIFIED BUT HOW THEY WORK AT A MECHANISTIC LEVEL AND CONTRIBUTE TO DISEASE PATHOGENESIS REMAINS UNCLEAR. MOREOVER, DESPITE HAVING COMMON CO- OCCURRING PATHOLOGY, LITTLE IS KNOWN ABOUT THE RELATIONSHIP BETWEEN THESE PROTEINOPATHIES, COMPLICATING POTENTIAL TREATMENT OPTIONS. RECENT EVIDENCE HAS SHOWN A LINK BETWEEN THE MITOCHONDRIAL-MEDIATED CLEARANCE OF AGGREGATED PROTEINS AND THE PRESERVATION OF NEURONAL CELL INTEGRITY. LIKEWISE, ESTABLISHED MODEL SYSTEMS OF PROTEINOPATHIES HAVE BOTH DEMONSTRATED SIMILAR RESULTS AND CARVED A WAY TO STUDY THE MOLECULAR DETAILS OF THESE DISEASES. IN THIS GRANT, WE PROPOSE TO THE BUDDING YEAST S. CEREVISIAE AND MAMMALIAN CELLS INCLUDING NEURONS TO ADDRESS THE ROLE OF MITOCHONDRIA AND CYCLIN C IN THE CLEARANCE OF THE AGGREGATED DISEASE PROTEINS. OF THESE PROTEINS TDP-43, HAS EMERGED AS A KEY PLAYER IN AD, ALS, AND FTLD. STUDIES IN YEAST HAVE REVEALED THAT CYCLIN C, A CONSERVED MEMBER OF THE CDK8 KINASE MODULE OF THE MEDIATOR COMPLEX, PLAYS A ROLE IN TDP-43 TOXICITY. OUR STUDIES POINT TO CYCLIN C’S KNOWN INTERACTION WITH MITOCHONDRIA, CONTRIBUTING TO TDP-43 MEDIATED-CELL DEATH. IN THIS PROPOSAL, WE WILL DETERMINE THE MOLECULAR DETAILS OF THE RELATIONSHIP BETWEEN CYCLIN C AND TDP-43, MITOCHONDRIA, AND CELL VIABILITY IN YEAST (AIM 1) AND MAMMALIAN SYSTEMS (AIM 2). TOGETHER THESE STUDIES SHOULD PROVIDE INSIGHT INTO THE ROLE CYCLIN C PLAYS IN PROTEINOPATHIES.

COUNTERACTING CARDIORESPIRATORY AND SUBJECTIVE EFFECTS OF FENTANYL-XYLAZINE MIXTURES USING A DUAL ANTAGONIST APPROACH - PROJECT SUMMARY SINCE THE MID-2010S, THE US HAS EXPERIENCED A SHARP RISE IN DRUG OVERDOSE FATALITIES DRIVEN BY THE EMERGENCE OF THE ULTRA-POTENT SYNTHETIC OPIOID FENTANYL WITHIN THE ILLICIT DRUG SUPPLY. RECENTLY, EVIDENCE DERIVED FROM DRUG SEIZURES, EPIDEMIOLOGICAL SURVEYS, AND CLINICAL CASE REPORTS SHOW THAT FENTANYL IS BEING INCREASINGLY ADULTERATED WITH XYLAZINE, AN ALPHA-2 ADRENERGIC RECEPTOR AGONIST APPROVED FOR VETERINARY USE AS A SEDATIVE AND ANALGESIC. ACCORDING TO ANECDOTAL REPORTS, XYLAZINE IS A DESIRABLE ADULTERANT BECAUSE IT PROLONGS THE OTHERWISE BRIEF EUPHORIA PRODUCED BY FENTANYL. HOWEVER, FENTANYL-XYLAZINE MIXTURES HAVE ALSO BEEN ASSOCIATED WITH SEVERE HEALTH RISKS AS COMPARED TO FENTANYL ALONE, INCLUDING POTENTIATED DRUG-INDUCED HYPOXIA AND ENHANCED RESISTANCE TO THE OPIOID RECEPTOR ANTAGONIST NALOXONE. COLLECTIVELY, THESE INTERACTIVE EFFECTS LIKELY CONTRIBUTE TO THE GREATER OVERALL RISK FOR IRREVERSIBLE OVERDOSE AND LETHALITY RECENTLY OBSERVED IN PRECLINICAL STUDIES AND CLINICAL CASE REPORTS. THIS HAS LED THE US GOVERNMENT TO DESIGNATE FENTANYL-XYLAZINE MIXTURES AS A “HIGHLY TOXIC CHEMICAL OF CONCERN” AND AN “EMERGING THREAT” REQUIRING RAPID RESPONSE. ALPHA-2 RECEPTOR ACTIVATION REDUCES CENTRAL NOREPINEPHRINE LEVELS, RESULTING IN A PHYSIOLOGICAL TOXIDROME RESEMBLING OPIOID EXPOSURE THAT INCLUDES RESPIRATORY DEPRESSION, BRADYCARDIA, AND HYPOTENSION. HOWEVER, THE PRECISE IMPACT OF FENTANYL-XYLAZINE MIXTURES ON CARDIORESPIRATORY FUNCTION HAS NOT BEEN EXAMINED, LEAVING UNRESOLVED THE SPECIFIC MANNER BY WHICH LETHALITY MIGHT BE PREVENTED IN AN EMERGENCY MEDICAL SETTING. FURTHERMORE, XYLAZINE-INDUCED POTENTIATION OF FENTANYL’S SUBJECTIVE EFFECTS HAS NOT YET BEEN EVALUATED IN ANIMAL MODELS, AND THUS THE PHARMACOLOGICAL MECHANISMS UNDERLYING THIS INTERACTIVE EFFECT REMAIN POORLY UNDERSTOOD. IN THIS PROPOSAL, WE WILL TEST THE HYPOTHESIS THAT XYLAZINE ENHANCES BOTH THE CARDIORESPIRATORY-DEPRESSANT AND SUBJECTIVE EFFECTS OF FENTANYL, AND THAT DUAL ANTAGONISM OF OPIOID RECEPTORS AND ALPHA-2 ADRENERGIC RECEPTORS PREVENTS THE EFFECTS OF COMBINED FENTANYL-XYLAZINE EXPOSURE ON THESE MEASURES IN RATS. IN AIM 1, WE WILL USE TELEMETRY AND PLETHYSMOGRAPHY TO COMPREHENSIVELY CHARACTERIZE XYLAZINE’S IMPACTS ON FENTANYL-INDUCED CHANGES IN HEART RATE, BLOOD PRESSURE, CORE TEMPERATURE, AND RESPIRATION. IN AIM 2, WE WILL DETERMINE WHETHER XYLAZINE INTENSIFIES AND/OR PROLONGS THE INTEROCEPTIVE-STIMULUS EFFECTS OF FENTANYL USING DRUG DISCRIMINATION PROCEDURES. FINALLY, IN BOTH AIMS, WE WILL TEST WHETHER THE EFFECTS OF FENTANYL-XYLAZINE MIXTURES ARE FULLY ANTAGONIZED BY CO-ADMINISTRATION OF NALOXONE AND THE HIGHLY-SELECTIVE ALPHA-2 RECEPTOR ANTAGONIST ATIPAMEZOLE, AN FDA-APPROVED VETERINARY MEDICATION THAT SAFELY AND EFFECTIVELY REVERSES THE SEDATIVE AND CARDIORESPIRATORY-DEPRESSANT EFFECTS OF ALPHA-2 RECEPTORS AGONISTS IN HUMANS. IF SUCCESSFUL, THESE STUDIES WILL IDENTIFY THE RECEPTOR TARGETS THAT MEDIATE BOTH THE DESIRABLE AND HARMFUL EFFECTS OF FENTANYL-XYLAZINE MIXTURES, PAVING THE WAY FOR THE RAPID DEVELOPMENT OF EFFECTIVE OVERDOSE RESCUE MEDICATIONS AND MUCH-NEEDED TREATMENTS FOR FENTANYL-XYLAZINE MISUSE, POTENTIALLY SAVING THOUSANDS OF LIVES.

IMPACT AID PROGRAM, TITLE VII, SECTION 7003

PREFRONTAL NEURAL MODULATION TO RESTORE COGNITIVE DEFICITS IN AN ALZHEIMER'S DISEASE RAT MODEL - PROJECT SUMMARY ALZHEIMER’S DISEASE (AD) IS CHARACTERIZED BY MEMORY IMPAIRMENTS AND UNDERLYING NEUROPATHOLOGY, INCLUDING PLAQUES, AMYLOID-SS PEPTIDES, TAU, IN CONJUNCTION WITH NEUROINFLAMMATION, AND NEURONAL INJURY/LOSS, PARTICULARLY IN THE MEDIAL TEMPORAL LOBE. RECENT STUDIES, HOWEVER, SUGGEST NEUROPHYSIOLOGICAL ALTERATIONS IN THE PREFRONTAL CORTEX (PFC) EVIDENT IN AD PATIENTS PRIOR TO GROSS NEUROPATHOLOGY WHICH MAY CONTRIBUTE TO DEFICITS IN COGNITIVE PROCESSING EARLY IN AD. GIVEN THAT THE PFC CONTRIBUTES TO TOP-DOWN CONTROL OF MEMORY PROCESSING NECESSARY FOR OPTIMAL DECISION-MAKING, DYSFUNCTION WITHIN THE PFC MAY CONTRIBUTE TO SUBOPTIMAL DECISION-MAKING WHICH OFTEN PRECEDES GROSS MEMORY LOSS IN AD PATIENTS. OPTIMAL DECISION-MAKING REQUIRES FUNCTIONING WORKING MEMORY PROCESSES (I.E., USING INFORMATION “ONLINE” TO GUIDE CHOICES) AND FLEXIBLE BEHAVIOR (THE ABILITY TO SHIFT BEHAVIOR IN RESPONSE TO CHANGING CONSEQUENCES). CRITICALLY, EARLY-STAGE AD MAY TARGET BRAIN CIRCUITS THAT UNDERLIE THESE PREFRONTAL DEPENDENT PROCESSES LEADING TO EARLY IMPAIRMENT IN OPTIMAL COGNITIVE PROCESSING. AS SUCH, UNDERSTANDING NEURAL CIRCUITS THAT ARE AFFECTED IN AD UNDERLIE COMPLEX COGNITIVE DEFICITS MAY LEAD TO EARLIER AND EFFECTIVE SCREENING FOR AD RISK AND SERVE AS AN IMPORTANT THERAPEUTIC TARGET FOR IMPROVING THE QUALITY OF LIFE AD PATIENTS. THE RAT PRELIMBIC CORTEX (PRL) IS HEAVILY IMPLICATED IN WORKING MEMORY AND FLEXIBLE BEHAVIOR NECESSARY FOR ONLINE DECISION-MAKING. AD RATS THAT WERE DEVELOPED TO SHOW AGE-DEPENDENT NEUROPATHOLOGICAL SIGNATURES (PLAQUES, TAU) CONSISTENT WITH AD ARE IMPAIRED IN PRL-DEPENDENT COGNITIVE TASKS PRIOR TO ACCUMULATION OF NEUROPATHOLOGY. CRITICALLY, THESE DEFICITS IN PRL-DEPENDENT TASKS PRECEDE BEHAVIORAL DEFICITS IN OTHER MEMORY TASKS THAT DO NOT DEPEND ON PRL FUNCTION. AS SUCH, TARGETING THE PRL FUNCTION IN VIVO MAY RESTORE THESE COGNITIVE PROCESSES IN THE AD RAT MODEL. HERE, WE AIM TO USE NONINVASIVE BRAIN STIMULATION THAT MODULATES NEURAL OSCILLATIONS TO RESTORE PRL-HIPPOCAMPAL NEURAL ACTIVITY (AIM 1) AND PRL-ORBITOFRONTAL CORTEX NEURAL ACTIVITY (AIM 2) IN WORKING MEMORY (DELAY NONMATCH TO POSITION TASK) AND FLEXIBLE BEHAVIOR (REVERSAL LEARNING), RESPECTIVELY.

IMPACT AID PROGRAM TITLE VIII SECTION 8003

INDIAN EDUCATION FORMULA GRANTS TO LEAS

IMPACT OF STRESS-INDUCED CIRCUIT-SPECIFIC CHANGES IN LOCUS COERULEUS OPIOID SIGNALING ON ANXIETY-LIKE BEHAVIOR

PRODUCTIVE AND LATENT HIV INFECTION OF MICROGLIA: VIRUS AND HOST WRESTLE FOR SUMOYLATION SYSTEM CONTROL - PROJECT SUMMARY THIS PROJECT WILL EXPLORE STRATEGIES TO TARGET EPIGENETIC PATHWAYS FOR ACHIEVING SUSTAINED HIV REMISSION AND TREATMENT OF HIV ASSOCIATED-CENTRAL NERVOUS SYSTEM (CNS) DYSFUNCTION. THE CONJUGATION OF SMALL UBIQUITIN- SUMO PROTEINS TO SUBSTRATES IS A WELL-DESCRIBED POST-TRANSLATIONAL MODIFICATION THAT REGULATES PROTEIN ACTIVITY, SUBCELLULAR LOCALIZATION, AND PROTEIN-PROTEIN INTERACTIONS FOR VARIOUS DOWNSTREAM ACTIVITIES. SUMO PROTEINS ARE ALSO IMPORTANT IN ANTI-VIRAL IMMUNITY, OPPOSING VIRAL REPLICATION AND MEDIATING INTERFERON-DEPENDENT ANTI-VIRAL MECHANISMS. THUS, PATHOGENS HAVE EVOLVED TO EXPLOIT THE HOST SUMOYLATION MACHINERY TO ENSURE VIRAL PERSISTENCE AND PATHOGENESIS. DURING INFECTION, THE HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 (HIV-1) MANIPULATES THE HOST SUMOYLATION MACHINERY TO ENSURE ITS VIRAL REPLICATION IN CD4+ MANIPULATES MICROGLIA T CELLS, HOWEVER, WHETHER HIV-1 THE SUMO PARALOGS TO CONTROL ITS REPLICATION AND/OR ATENCY IN GLIAL CELLS LIKE MICROGLIA IS UNCLEAR. ARE THE MAIN HIV-1 TARGET CELLS IN THE CNS AND CONSTITUTE AN IMPORTANT RESERVOIR FOR VIRAL PATHOGENESIS. L IN MICROGLIAL CELLS, THE CO-REPRESSOR COUP-TF INTERACTING PROTEIN 2 (CTIP2) RECRUITS A MULTI-ENZYMATIC CHROMATIN- MODIFYING COMPLEX AND ESTABLISHES A HETEROCHROMATIC ENVIRONMENT AT THE HIV-1 PROMOTER, LEADING TO HIV-1 SILENCING. STUDIES HAVE SHOWN THAT POST-TRANSLATIONAL MODIFICATIONS (PTMS), INCLUDING PHOSPHORYLATION AND SUMOYLATION, MEDIATE CTIP2'S INTERACTIONS WITH OTHER PROTEINS AND COMPLEXES. SIMILARLY, TRIPARTITE MOTIF- CONTAINING PROTEIN 28 (TRIM28), A KNOWN SUMO E3 LIGASE, ASSOCIATES WITH CTIP2 IN THE HETEROCHROMATIN COMPLEX TO INHIBIT HIV-1 VIRAL REPLICATION. WHILE REGULATING RESPECTIVELY, UNKNOWN. PRODUCTIVE SUMOYLATION INDUCIBLE SUMOYLATION AND PHOSPHORYLATION HAVE BEEN IMPLICATED IN THE ACTIVITY OF CTIP2 AND TRIM28 IN THE CONTEXT OF T-CELL SIGNALING EVENTS AND IMMUNE RESPONSES, THE IMPACT OF PTMS IN THE INITIATION AND ESTABLISHMENT OF HIV-1 LATENCY IN MICROGLIA REMAINS TO THIS END, THIS PROPOSAL'S CENTRAL HYPOTHESIS IS THAT THE HOST SUMOYLATION SYSTEM IS I MPAIRED DURING HIV-1 INFECTION IN MICROGLIA AND THAT LATENCY IS ESTABLISHED AS A RESULT OF THE ESTORATION OF OF HOST PROTEINS. TO TEST THIS HYPOTHESIS , WE WILL USE IMMORTALIZED HUMAN MICROGLIAL CELL LINES AND PLURIPOTENT STEM CELLS (IPSCS), INCLUDING A NOVEL MODEL OF HIV-1 LATENCY. R WE WILL ALSO IDENTIFY THE SUMO-MODIFIED PROTEOME OF HUMAN MICROGLIAL CELLS DURING HUMAN HIV-1 INFECTION.

FORMULA GRANTS TO LEAS

RNA OXIDATION IN RAS-DRIVEN CANCER - ABSTRACT CHEMICAL MODIFICATIONS IN RNA PLAY A CRUCIAL ROLE IN REGULATING GENE EXPRESSION IN PHYSIOLOGICAL AND PATHOLOGICAL CONDITIONS. ONE UNDEREXPLORED ASPECT OF THIS REGULATION COMES FROM REACTIVE OXIDATIVE SPECIES (ROS), WHICH MODIFY NUCLEOBASES IN RNA. ELEVATED LEVELS OF ROS AND OXIDATIVE STRESS ARE PREVALENT IN CANCER CELLS, RESULTING FROM METABOLIC REPROGRAMMING AND DRIVEN BY FACTORS SUCH AS ACTIVATED RAS EXPRESSION. HOWEVER, OUR UNDERSTANDING OF HOW ROS-INDUCED RNA MODIFICATIONS AFFECT GENE EXPRESSION IN A CANCER CONTEXT REMAINS LIMITED. THIS STUDY AIMS TO FILL THIS KNOWLEDGE GAP BY EXPLORING THE ROLES AND REGULATORY IMPLICATIONS OF OXIDATIVE MODIFICATIONS OF RNA, WITH A FOCUS ON KRAS-DRIVEN REDOX PERTURBATIONS IN NON-SMALL CELL LUNG CANCER CELLS. THE PROJECT HAS TWO MAIN OBJECTIVES. THE FIRST OBJECTIVE IS TO IDENTIFY THE PATTERNS OF OXIDATIVE MODIFICATIONS IN RNA INDUCED BY ONCOGENIC KRAS. THIS WILL BE ACHIEVED THROUGH THE COMBINATION OF NEXT-GENERATION SEQUENCING TECHNIQUES AND CHEMICAL METHODS TO CREATE HIGH-RESOLUTION PROFILES OF 8-OXOGUANINE MODIFICATIONS IN RNA. THE SECOND OBJECTIVE IS TO UNDERSTAND THE MOLECULAR PLAYERS THAT ‘READ’ THESE MODIFICATIONS. THIS WILL BE DONE THROUGH BIOCHEMICAL AND CLIP ANALYSIS TECHNIQUES, FOCUSING ON PROTEINS FROM THE HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN (HNRNP) FAMILY. BY ELUCIDATING THESE INTERACTIONS, THE STUDY AIMS TO UNCOVER POTENTIAL INFLUENCES OF OXIDATIVE RNA MODIFICATIONS ON GENE EXPRESSION CHANGES IN CANCER CELLS. THE OUTCOMES OF THIS STUDY WILL SIGNIFICANTLY CONTRIBUTE TO OUR FUNDAMENTAL UNDERSTANDING OF GENE REGULATION IN CANCER. SINCE OXIDATIVE STRESS IS A HALLMARK OF MANY PATHOLOGICAL STATES, THE TECHNOLOGICAL AND CONCEPTUAL ADVANCEMENTS MADE IN THIS STUDY WILL ALSO HAVE BROADER RELEVANCE IN UNDERSTANDING THE ROLE OF OXIDATIVE RNA MODIFICATIONS IN THE PATHOGENESIS OF DISEASES.

STRESS INDUCED SIGNALING AND CYCLIN C REGULATION IN YEAST

IMPACT AID PROGRAM, TITLE VII, SECTION 7003

IMPACT AID PROGRAM TITLE VIII SECTION 8003

INDIAN EDUCATION FORMULA GRANTS TO LEAS

FORMULA GRANTS TO LEAS

INDIAN EDUCATION FORMULA GRANTS TO LEAS

CELLULAR AND MOLECULAR MECHANISMS REGULATING SYNOVIAL JOINT DEVELOPMENT - PROJECT SUMMARY AND ABSTRACT SYNOVIAL JOINTS ARE ESSENTIAL FOR BODY MOTION AND QUALITY OF LIFE. THEIR SYNOVIAL CAVITY AND LUBRICANT-RICH FLUID PERMIT UNHINDERED JOINT MOTION AND FUNCTION AND PROVIDE TISSUE PROTECTION AND NOURISHMENT. WHILE THESE ASPECTS OF SYNOVIAL JOINT BIOLOGY ARE WELL UNDERSTOOD, LITTLE IS KNOWN ABOUT HOW THE CAVITY AND ITS FLUID ACTUALLY DEVELOP DURING EMBRYOGENESIS. AT EARLY FETAL STAGES, THE LIMB SKELETAL PRIMORDIA ARE COMPOSED OF CONTINUOUS CARTILAGINOUS STRUCTURES WITHOUT JOINTS. JOINT DEVELOPMENT STARTS WITH APPEARANCE OF AN “INTERZONE”, A TISSUE MADE OF MESENCHYMAL CELLS EXPRESSING THE GROWTH AND DIFFERENTIATION FACTOR 5 GENE (GDF5). WE PREVIOUSLY SHOWED THAT GDF5+ CELL PROGENIES PRODUCE MOST JOINT TISSUES OVER TIME AND THE SYNOVIAL CAVITY FORMS IN THE MIDDLE OF THE INTERZONE. BECAUSE THE INTERZONE CELLS ARE INITIALLY ATTACHED TO EACH OTHER, THE CAVITATION PROCESS MUST INVOLVE THEIR PHYSICAL SEPARATION ALONG THE PROSPECTIVE ARTICULAR LINE TO FACILITATE THE CREATION OF A FLUID-FILLED CAVITY. PREVIOUS STUDIES INDICATED THAT INTERZONE CELLS PRODUCE HYALURONAN (HA) AROUND THE CAVITATION TIME, AND THIS IS ACCOMPANIED BY ACCUMULATION OF A HA-RICH MATRIX IN LOCAL TISSUES. HA IS A MAJOR COMPONENT OF EXTRACELLULAR MATRIX AND SYNOVIAL FLUID AND PLAYS IMPORTANT ROLES IN TISSUE HOMEOSTASIS. IN MY PRELIMINARY STUDIES, I FOUND THAT JUST BEFORE CAVITATION ONSET, INTERZONE CELLS IN MOUSE EMBRYO LIMBS EXPRESS HYALURONAN SYNTHASE 2 (HAS2, ‘THE HA SYNTHESIZER’) AND TRANSMEMBRANE PROTEIN 2 (TMEM2), A CELL SURFACE HYALURONIDASE THAT SPECIFICALLY CLEAVES HIGH MOLECULAR WEIGHT HA INTO INTERMEDIATE AND BIOLOGICALLY-ACTIVE FRAGMENTS. I ALSO DISCOVERED THAT, MORPHOLOGICALLY, CAVITATION INITIATES WITH FORMATION OF MICROLUMENS ALONG THE PROSPECTIVE ARTICULAR LINE AND IS COMPLETED SOON AFTERWARDS WHEN THE POCKETS COALESCE TO GENERATE A SINGLE ONE SYNOVIAL CAVITY. THIS PROCESS IS EXTREMELY RAPID IN THE DEVELOPING KNEE BUT IS SLOWER IN DIGITS. THESE AND OTHER NOVEL DATA LEAD TO MY CENTRAL HYPOTHESIS THAT JOINT CAVITATION IS BROUGHT ABOUT BY CONVERGENCE OF DIVERSE BUT COORDINATED BIOLOGICAL PROCESSES. ACCORDINGLY, AIM 1 IS TO DETERMINE THE ROLE OF HAS2 AND TMEM2 IN JOINT CAVITATION USING GENETICALLY MODIFIED MOUSE MODELS. I WILL CONDITIONALLY DELETE HAS2 AND/OR TMEM2 IN INTERZONE CELLS (USING GDF5CRE MICE) AND SUBJECT RESULTING MUTANT EMBRYOS TO DETAILED ANALYSIS. AIM 2 IS TO DETERMINE CELLULAR AND MOLECULAR MECHANISMS OF CAVITATION. I WILL INVESTIGATE DOWNSTREAM SIGNALING PATHWAYS IN RESPONSE TO CHANGES IN HA SIZES AND RESULTING INTERACTIONS WITH CELL SURFACE CD44 RECEPTOR, REGULATING HA METABOLISM IN SYNOVIAL JOINT DEVELOPMENT AND LONG-TERM MAINTENANCE. THE PROJECT WILL PROVIDE NOVEL INSIGHTS INTO MECHANISMS UNDERLYING JOINT DEVELOPMENT AND CAVITATION. IN LINE WITH THE K01 MECHANISM, THE PROJECT WILL ALLOW ME TO ACQUIRE NEW EXPERTISE IN SKELETAL DEVELOPMENTAL AND MOLECULAR BIOLOGY AND TO INTEGRATE IT WITH MY PREVIOUS TRAINING IN BIOENGINEERING. THIS UNIQUE COMBINATION OF EXPERTISE IN TWO DISTINCT BUT INTERRELATED FIELDS WILL ALLOW ME TO ESTABLISH AN INDEPENDENT CAREER DISTINCT FROM MY MENTORS AND CREATE NOVEL THERAPEUTIC TOOLS TO REPAIR AND REGENERATE CARTILAGE FOR THE TREATMENT OF JOINT CONDITIONS SUCH AS OSTEOARTHRITIS (OA).

IMPACT AID PROGRAM, TITLE VII, SECTION 7003

IMPACT AID PROGRAM TITLE VIII SECTION 8003 AND SECTION 8007(A)

CHP

UNKNOWN TITLE

IMPACT AID PROGRAM, TITLE VII, SECTION 7003

PREDICTING MALADAPTIVE AVERSIVE LEARNING VIA COMPUTATIONAL MODELING OF INSULAR SINGLE CELL ENSEMBLE ACTIVITY PATTERNS - PROJECT SUMMARY ANXIETY DISORDERS SUCH AS PANIC DISORDERS, GENERALIZED ANXIETY DISORDER, AND POST-TRAUMATIC STRESS DISORDER (PTSD) AFFECT APPROXIMATELY 18% OF THE AMERICAN POPULATION WITH A HEALTH CARE COST OF MORE THAN $42 BILLION A YEAR, A SIGNIFICANT BURDEN TO THE US ECONOMY. DEVELOPMENT AND MAINTENANCE OF ANXIETY DISORDERS HAVE BEEN ATTRIBUTED TO PERSISTENT FEAR MEMORIES, INADEQUATE FEAR EXTINCTION, AND MALADAPTIVE AVOIDANCE BEHAVIOR. THUS, IT IS IMPERATIVE TO UNDERSTAND THE NEURAL MECHANISMS UNDERLYING AVERSIVE LEARNING IN ORDER TO BE ABLE TO DEVELOP EFFICACIOUS TREATMENTS FOR THESE DISORDERS. IN THIS PROJECT, WE WILL FOCUS ON UNDERSTANDING THE INVOLVEMENT OF THE INSULA, A BRAIN REGION HEAVILY INVOLVED NOT ONLY IN AVERSIVE LEARNING IN GENERAL BUT ALSO PROCESSES DETERMINING APPROACH/AVOIDANCE BEHAVIORS. SPECIFICALLY, USING IN-VIVO SINGLE CELL CALCIUM IMAGING VIA MINISCOPES, WE WILL RECORD ACTIVITY PATTERNS OF INSULAR SINGLE CELL ENSEMBLES DURING FEAR LEARNING WHEN THE AVERSIVE OUTCOME (FOOTSHOCK) IS INESCAPABLE AS WELL AS WHEN THE AVERSIVE OUTCOME IS OMITTED (FEAR EXTINCTION; AIM1A) AND WHEN IT BECOMES ESCAPABLE (AVOIDANCE LEARNING; AIM1B). FINALLY, USING A NOVEL THEORETICAL-COMPUTATIONAL APPROACH TO FUNCTIONALLY CLUSTER FEAR LEARNING SINGLE CELL ENSEMBLES IN THE INSULA, WE WILL PREDICT WHETHER MICE WILL DEVELOP EXTINCTION RESISTANT FEAR OR IMPAIRED AVOIDANCE LEARNING (AIM2). THUS, IN THIS PROPOSAL, WE AIM TO INVESTIGATE THE INVOLVEMENT OF THE INSULAR SINGLE CELL ENSEMBLES IN AVERSIVE LEARNING AND DEVELOP A NOVEL COMPUTATIONAL TOOL TO PREDICT FUTURE MALADAPTIVE AVERSIVE LEARNING PHENOTYPES BASED ON THE NEURAL SIGNALING IN THE INSULA.

NEUROPLASTICITY-BASED SUICIDE PREVENTION PROGRAM FOR ALLOPATHIC AND OSTEOPATHIC MEDICAL STUDENTS AT ROWAN UNIVERSITY - ROWAN SCHOOL OF OSTEOPATHIC MEDICINE AND COOPER MEDICAL SCHOOL OF ROWAN UNIVERSITY PROPOSES A NEUROPLASTICITY-BASED SUICIDE PREVENTION PROGRAM THAT WILL ENGAGE 750 MEDICAL STUDENTS IN ACTIVITIES TO BUILD AWARENESS OF SUICIDE IN MEDICINE, PROVIDE TRAINING TO RESPOND TO STUDENTS IN DISTRESS, INCREASE ACCESS TO ONLINE SCREENING WITH COUNSELOR SUPPORT, AND OFFER NEUROSCIENCE-BASED WELLNESS TRAINING, PEER GROUPS, AND A NEURO-STIGMA ELIMINATION PROJECT. ROWAN UNIVERSITY SCHOOL OF OSTEOPATHIC MEDICINE (ROWANSOM) AND COOPER MEDICAL SCHOOL OF ROWAN UNIVERSITY (CMSRU) PROPOSE AN MD/DO HEALTHY MINDS PARTNERSHIP TO BUILD A COMPREHENSIVE SUICIDE PREVENTION PROGRAM THAT WILL ADDRESS HIGH RATES OF STRESS, BURNOUT, DEPRESSION, AND SUICIDAL THOUGHTS AMONG THEIR MEDICAL STUDENTS. ROWAN UNIVERSITY IS ONE OF ONLY TWO ACADEMIC INSTITUTIONS IN THE UNITED STATES THAT CONTAINS BOTH AN ALLOPATHIC AND AN OSTEOPATHIC MEDICAL SCHOOL. ALTHOUGH ROWANSOM OPERATES A MEDICAL STUDENT MENTAL HEALTH CLINIC THAT SERVES STUDENTS FROM BOTH SCHOOLS, THERE IS CURRENTLY NO UNIFIED SUICIDE PREVENTION FRAMEWORK BETWEEN THE MEDICAL SCHOOLS. THIS PROJECT IS A RESPONSE TO THREE SUICIDES INVOLVING STUDENTS ENROLLED AT ROWAN UNIVERSITY’S MAIN CAMPUS IN FALL 2019, A FATAL OVERDOSE IN 2021, AND RESULTS OF INTAKE ASSESSMENTS THAT SHOWED THAT 40% OF MEDICAL STUDENTS SEEKING MENTAL HEALTH SERVICES WERE MODERATELY OR SEVERELY DEPRESSED, AND 15% ENDORSED SUICIDAL THOUGHTS. THE GEOGRAPHIC CATCHMENT AREA WILL BE THREE MEDICAL SCHOOL CAMPUSES LOCATED IN CAMDEN AND GLOUCESTER COUNTIES OF NEW JERSEY. CMSRU IS LOCATED IN THE CITY OF CAMDEN, WHICH FACES ENORMOUS SOCIAL AND ECONOMIC CHALLENGES. ROWANSOM HAS TWO CAMPUSES, ONE IN STRATFORD, NEW JERSEY, WHICH IS A SUBURB IN CAMDEN COUNTY, AND ONE IN SEWELL, GLOUCESTER COUNTY, WHICH WILL BEGIN ADMITTING STUDENTS IN JULY 2022 AND MAKE ROWANSOM THE LARGEST MEDICAL SCHOOL IN NEW JERSEY. THE POPULATION OF FOCUS WILL BE MEDICAL STUDENTS AT CMSRU AND ROWANSOM. THE CURRENT ENROLLMENT AT ROWANSOM IS 850 STUDENTS, INCLUDING 6.7% AFRICAN AMERICAN, 8.2% HISPANIC, 40.5% WHITE, 39.5% ASIAN, AND 5.0% OTHER. FIFTY-ONE PERCENT OF ROWANSOM STUDENTS ARE FEMALE. CMSRU HAS 449 STUDENTS ENROLLED, INCLUDING 4.9% AFRICAN AMERICAN, 3.1% HISPANIC, 45.2% WHITE, 36% ASIAN, AND 8.2% OTHER. FIFTY-THREE PERCENT OF CMSRU STUDENTS ARE FEMALE. THE GOALS OF THIS PROJECT ARE TO 1) ENHANCE THE MENTAL HEALTH AND SUICIDE PREVENTION SERVICES ON THREE MEDICAL SCHOOL CAMPUSES AT ROWAN; 2) IMPLEMENT A NEUROSCIENCE-BASED WELLNESS TRAINING CURRICULUM THAT WILL IMPROVE LIFE SKILLS AND REDUCE SUICIDE RISK; 3) INCREASE STUDENT AND FACULTY READINESS TO IDENTIFY AND RESPOND TO MEDICAL STUDENTS IN DISTRESS; AND 4) INCREASE HELP-SEEKING BEHAVIORS AMONG MEDICAL STUDENTS. INNOVATIVE ACTIVITIES INCLUDE PROVIDING ACCESS TO A VOLUNTARY ONLINE INTERACTIVE SCREENING TOOL WITH WHICH MEDICAL STUDENTS CAN SCREEN THEMSELVES FOR MENTAL HEALTH CONDITIONS AND RECEIVE ONLINE COUNSELING SUPPORT, DEVELOPING A HEALTHY MINDS PARTNERSHIP CHALLENGE WHERE BOTH MEDICAL SCHOOLS WILL COMPETE WITH ONE ANOTHER TO DETERMINE WHICH SCHOOL CAN ENROLL THE HIGHEST NUMBER OF STUDENTS, FACULTY, AND STAFF IN SUICIDE PREVENTION TRAINING, IMPLEMENTING NEUROSCIENCE-BASED TRAINING ACTIVITIES TO PRODUCE BRAIN CHANGES THAT SUPPORT HUMAN FLOURISHING, ENGAGING STUDENTS INTO A PEERS SUPPORT GROUP, DEVELOPING A NEURO-STIGMA ELIMINATION PROJECT, AND IMPLEMENTING A CRISIS ACCESS AND RESPONSE EDUCATIONAL SYSTEM TO EDUCATE STUDENTS ON HOW TO ACCESS THE NATIONAL SUICIDE PREVENTION LIFELINE AND OTHER CRISIS SERVICES. EVALUATION OF THE PROGRAM WILL BE CONDUCTED BY RUTGERS UNIVERSITY.

IMPACT AID PROGRAM TITLE VIII SECTION 8003

PREVENTION OF OPIOID MISUSE AND OVERDOSE IN HIGH RISK WOMEN IN NEW JERSEY

IMPACT AID PROGRAM, TITLE VIII, SECTION 8003

CARES ACT - FIPSE FUNDS

UNKNOWN TITLE

UNKNOWN TITLE

EARMARKS

FY21 SCHOOL VIOLENCE PREVENTION PROGRAM (SVPP)

IMPACT AID PROGRAM, TITLE VIII, SECTION 8003

IMPACT AID PROGRAM, TITLE VIII, SECTION 8003

IMPACT AID PROGRAM, TITLE VIII, SECTION 8003

HEALTH CONSEQUENCES OF DISATER-RELATED DISRUPTIONS IN HOME AND COMMUNITY-BASED SUPPORTS

READINESS AND EMERGENCY MANAGEMENT FOR SCHOOLS

IMPACT AID PROGRAM, TITLE VIII, SECTION 8003

EXPANSION OF OSTEOPATHIC MEDICINE PRACTIONER EDUCATION ON SUBSTANCE USE DISORDERS

IMPACT AID PROGRAM, TITLE VII, SECTION 7003

IMPACT AID PROGRAM, TITLE VIII, SECTION 8003

IMPACT AID PROGRAM, TITLE VIII, SECTION 8003

CAROL M. WHITE PHYSICAL EDUCATION PROGRAM

NUCLEOLAR SIGNALING IN CANCER

ADVANCED TECHNOLOGY FOR ADVANCED MANUFACTURING

INDIAN EDUCATION FORMULA GRANTS TO LEAS

INDIAN EDUCATION FORMULA GRANTS TO LEAS

INDIAN EDUCATION FORMULA GRANTS TO LEAS

INDIAN EDUCATION FORMULA GRANTS TO LEAS

INDIAN EDUCATION FORMULA GRANTS TO LEAS

UNKNOWN TITLE

FORMULA GRANTS TO LEAS

FORMULA GRANTS TO LEAS

UNKNOWN TITLE

IMPACT AID SCHOOL CONSTRUCTION FORMULA GRANTS RECOVERY ACT

INDIAN EDUCATION FORMULA GRANTS TO LEAS

FORMULA GRANTS TO LEAS

UNKNOWN TITLE

FORMULA GRANTS TO LEAS

IMPACT AID PROGRAM TITLE VIII SECTION 8002

FORMULA GRANTS TO LEAS

IMPACT AID PROGRAM, TITLE VII, SECTION 7002

IMPACT AID PROGRAM, TITLE VII, SECTION 7002

IMPACT AID PROGRAM, TITLE VII, SECTION 7002

INDIAN EDUCATION FORMULA GRANTS TO LEAS

IMPACT AID PROGRAM, TITLE VII, SECTION 7002

IMPACT AID PROGRAM, TITLE VII, SECTION 7002

UNKNOWN TITLE

UNKNOWN TITLE

IMPACT AID PROGRAM, TITLE VII, SECTION 7002

FORMULA GRANTS TO LEAS

UNKNOWN TITLE

IMPACT AID PROGRAM TITLE VIII SECTION 8002

IMPACT AID PROGRAM, TITLE VIII, SECTION 8002

IMPACT AID PROGRAM, TITLE VIII, SECTION 8002

FORMULA GRANTS TO LEAS

IMPACT AID PROGRAM TITLE VIII SECTION 8002

IMPACT AID PROGRAM, TITLE VIII, SECTION 8002

FORMULA GRANTS TO LEAS

IMPACT AID PROGRAM, TITLE VIII, SECTION 8002

DRUG FREE COMMUNITIES SUPPORT PROGRAM

UNKNOWN TITLE

PSYCHOSTIMULANT EFFECTS ON COGNITIVE FLEXIBILITY AND RISK-BASED DECISION-MAKING BEHAVIOR FOLLOWING REPETITIVE MILD TRAUMATIC BRAIN INJURY

IMPACT AID PROGRAM, TITLE VIII, SECTION 8002

IMPACT AID PROGRAM TITLE VIII SECTION 8002

GERIATRICS WORKFORCE ENHANCEMENT PROGRAM COVID

AREA HEALTH EDUCATION CENTERS PROGRAM COVID

IMPACT AID PROGRAM, TITLE VIII, SECTION 8002

ENHANCING SCHOOL SAFETY USING A THREAT ASSESSMENT MODEL

INDIAN EDUCATION FORMULA GRANTS TO LEAS

SMALL, RURAL SCHOOL ACHIEVEMENT PROGRAM

INDIAN EDUCATION FORMULA GRANTS TO LEAS

INDIAN EDUCATION FORMULA GRANTS TO LEAS

INDIAN EDUCATION FORMULA GRANTS TO LEAS

INDIAN EDUCATION FORMULA GRANTS TO LEAS

INDIAN EDUCATION FORMULA GRANTS TO LEAS