Burke Ltd

MITOCHONDRIAL DYSFUNCTION IN NEUROGEGENERATION OF AGING

EARLY HEAD START

BURKE COUNTY BOARD OF EDUCATION EARLY HEAD START IS A COMPREHENSIVE EARLY CHILD DEVELOPMENT AND FAMILY SUPPORT PROGRAM FOR LOW INCOME FAMILIES WITH C

NEURAL PREDICTORS OF HAND THERAPY EFFICACY IN CHILDREN WITH CEREBRAL PALSY

SAFE SCHOOLS/HEALTHY STUDENTS GRANTS

PLASTICITY IN THE AGING OLFACTORY SYSTEM

SYNAPSE ELIMINATION IN THE CENTRAL NERVOUS SYSTEM

PA-25; PA-26

ELECTROPHYSIOLOGICAL EVALUATION OF BRAIN REGIONS VULNERABLE TO ALZHEIMERS DISEASE

BURKE'S CERTIFIED COMMUNITY BEHAVIORAL HEALTH CLINIC IMPROVEMENT AND ADVANCEMENT GRANT - BURKE’S FY 2023 CERTIFIED COMMUNITY BEHAVIORAL HEALTH CLINIC – IMPROVEMENT AND ADVANCEMENT (CCBHC-IA) GRANT PROGRAM ENDEAVORS TO IMPROVE, ENHANCE, AND EXPAND CAPACITY FOR OUR CHRONIC CARE MANAGEMENT (CCM) MODEL WITHIN THE EAST TEXAS REGION. THIS PROJECT SHALL TARGET ADULTS WITH SERIOUS MENTAL ILLNESS (SMI), CHILDREN AND YOUTH WITH SERIOUS EMOTIONAL DISTURBANCE (SED), AND INDIVIDUALS WITH CO-OCCURRING PSYCHIATRIC DIAGNOSES, SUBSTANCE USE DISORDERS (SUD), AND/OR PHYSICAL HEALTH DISORDERS IN OUR TWELVE COUNTIES: ANGELINA, HOUSTON, JASPER, NACOGDOCHES, NEWTON, POLK, SABINE, SAN AUGUSTINE, SAN JACINTO, SHELBY, TRINITY, AND TYLER. THE CCBHC-IA PROGRAM SHALL SERVE 600 INDIVIDUALS EACH YEAR, WITH A CUMULATIVE 2250 UNDUPLICATED INDIVIDUALS SERVED OVER THE PROJECT PERIOD. THROUGH USE OF THESE FUNDS, BURKE SHALL EXPAND CURRENT SERVICE DELIVERY CAPACITY FOR INTEGRATED BEHAVIORAL HEALTH AND PRIMARY CARE, PHYSICAL SCREENING, CARE MANAGEMENT, AND POPULATION HEALTH MONITORING BY ADDING 0.5 FTE MEDICAL DOCTOR, 3.0 FTE NURSES, 5.0 FTE CARE MANAGERS, 1.0 FTE APPLICATIONS PROGRAMMER, AND 0.5 FTE POPULATION HEALTH COORDINATOR. THESE STAFF SHALL REACH THE HISTORICALLY UNDER-SERVED TARGET POPULATION, REDUCE SOCIAL DETERMINANTS OF HEALTH (SDOHS) RELATED TO ECONOMIC INSTABILITY AND ACCESS TO CARE, AND BUILD UPON CURRENT CARE COORDINATION PROJECTS. PROJECT STAFF WILL FOCUS ON MEASURABLE OBJECTIVES, IMPLEMENT PROVISIONS TO IMPROVE POPULATION HEALTH INDICATORS, SUCH AS HIGH BLOOD PRESSURE, BMI, AND UNHEALTHY ALCOHOL USE, REDUCE ACCESS TO PHYSICAL CARE BY 10% AND TRAUMA SYMPTOMOLOGY BY 10% IN THE HISPANIC SUBPOPULATION OF FOCUS, IMPLEMENT NETSMARTTM CAREMANAGER SOFTWARE FOR RISK STRATIFICATION, AND DEVELOP A NEEDS ASSESSMENT AND SUSTAINABILITY PLAN. BURKE SHALL FORMALLY PARTNER WITH BROWN FAMILY HEALTH CENTER, INC. AS ITS DESIGNATED COLLABORATING ORGANIZATION (DCO) FOR THIS PROJECT, TO PROVIDE PRIMARY CARE AND INFECTIOUS DISEASE VACCINATION. THE MEDICAL DOCTOR AND NURSES SHALL WORK TO CONDUCT PHYSICAL HEALTH AND INFECTIOUS DISEASE SCREENINGS, CHRONIC DISEASE PREVENTION, AND REFERRALS. THE CARE MANAGERS SHALL COORDINATE CONSUMERS ENROLLED ON THE MEDICAL DOCTOR’S CASELOAD TO IMPROVE RETENTION, MAINTENANCE, AND DAILY FUNCTIONING POST-PROJECT. THE APPLICATIONS PROGRAMMER AND POPULATION HEALTH COORDINATOR SHALL WORK ON CAREMANAGER IMPLEMENTATION AND EXECUTION, HEALTH INFORMATION TECHNOLOGY (HIT) INTEROPERABILITY, AND ENDEAVOR TO MONITOR HEALTH OUTCOMES AND INEQUITY ACROSS THE PROJECT TIMELINE TO ADDRESS APPROPRIATE INTERVENTIONS WHEN NEEDED.

EARLY HEAD START

THE ROLE OF CD36 IN ISCHEMIC INFLAMMATION AND INJURY

CORTICOSPINAL NEURON DYSFUNCTION AND DEGENERATION IN ALS: TESTING THE ROLE OF CORTICOMOTOR CONNECTIVITY IN MOTOR NEURON DISEASE - ABSTRACT (SUMMARY): IN PATIENTS WITH AMYOTROPHIC LATERAL SCLEROSIS (ALS) AND THE RELATED MOTOR NEURON DISEASE (MND) PRIMARY LATERAL SCLEROSIS (PLS), DEFICITS IN MOTOR CONTROL OCCUR AS A CONSEQUENCE OF THE DEGENERATION OF CORTICOSPINAL NEURONS (CSNS). ALS IS MORE COMMON THAN PLS, AND GENETICALLY MORE COMPLEX, WITH FAMILIAL FORMS ASSOCIATED WITH CAUSAL MUTATIONS IN OVER 30 ALS-RELATED GENES. IN THESE ALS MICE, HOWEVER, DYSFUNCTION AND DEGENERATION OF CSNS HAVE NOT BEEN CAREFULLY EXAMINED, AND DATA IMPLICATING CORTICOSPINAL (CS) CIRCUITS IN THESE MODEL SYSTEMS OF ALS IS SURPRISINGLY LIMITED. ONE REASON FOR THIS MAY BE THE VERY DIFFERENT PATTERN OF CONNECTIVITY BETWEEN CSNS AND SPINAL MNS IN HUMANS VS. MICE. IN HUMANS, CS AXONS LOCATED IN THE VENTRAL AND LATERAL FUNICULI FORM DIRECT CONNECTIONS WITH BOTH MNS (CORTICO-MOTONEURONAL (CM) CONNECTIONS) AND INTERNEURONS. IN CONTRAST, CS AXONS IN MICE ARE LOCATED MAINLY IN THE DORSAL FUNICULUS AND ONLY FORM INDIRECT CONNECTIONS WITH MNS THROUGH PRE-MOTOR INTERNEURONS. THEREFORE, WE WILL USE PLEXINA1 MUTANT MICE WHICH HAVE CM CONNECTIONS TOGETHER WITH ALS MOUSE MODELS TO ANALYZE CS CIRCUITS. OUR CENTRAL HYPOTHESIS IS THAT PROGRESSIVE DEFECTS IN CS CIRCUITRY IN ALS MICE WILL BE EXACERBATED BY THE ESTABLISHMENT OF CM CONNECTIONS. IN AIM 1, WE WILL DETERMINE FORMATION OF CS CIRCUITS IN ALS MOUSE MODELS WITH CM CONNECTIONS. IN AIM 2, WE WILL DETERMINE FUNCTION OF CS CIRCUITS IN ALS MOUSE MODELS WITH CM CONNECTIONS. IN AIM 3, WE WILL EXAMINE SKILLED MOVEMENTS IN ALS MOUSE MODELS WITH CM CONNECTIONS. THESE STUDIES WILL PROVIDE A MODEL SYSTEM TO STUDY MECHANISMS OF CS DEGENERATION IN ALS/PLS, AND TO TEST NOVEL THERAPEUTICS TARGETING UPPER MOTOR NEURON DYSFUNCTION IN THESE DISORDERS.

TRANSCRANIAL DIRECT CURRENT STIMULATION AND ROBOTIC TRAINING IN CHRONIC STROKE

ENGAGING NEURON-INTRINSIC SIGNALING FOR AXON GROWTH AFTER SPINAL CORD INJURY

GRADING VISUAL IMPAIRMENT IN CHILDREN WITH BRAIN INJURY

MODULATION OF CORTICAL NETWORKS, A NEW APPROACH TO SPINAL CORD INJURY REHABILITATION

IMMUNE-MEDIATED MECHANISMS UNDERLYING CONDITIONING-INDUCED STROKE RECOVERY

VASCULAR CONTROL IN DIABETIC RETINOPATHY

EHS FY09 ARRA EXAPNSION

B-RAF DRIVES REGENERATIVE AXON GROWTH IN THE OPTIC NERVE IN VIVO

RETINAL NEURAL PROCESSING DURING RETINAL DEGENERATIVE DISEASES

BURKE'S NATIONAL CHILD TRAUMATIC STRESS INITIATIVE - BURKE’S FY 2023 NATIONAL CHILD TRAUMATIC STRESS INITIATIVE – CATEGORY III: COMMUNITY TREATMENT AND SERVICE (CTS) CENTERS (NCTSI) GRANT PROGRAM ENDEAVORS TO IMPROVE, ENHANCE, AND EXPAND CAPACITY FOR TRAUMA AND GRIEF COUNSELING, CASE MANAGEMENT, AND CARE COORDINATION WITHIN THE EAST TEXAS REGION. THIS PROJECT SHALL TARGET UNDERSERVED AND UNDER-RESOURCED CHILDREN, ADOLESCENTS, AND THEIR FAMILIES WHO HAVE EXPERIENCED TRAUMA AND TRAUMATIC EVENTS IN TWELVE COUNTIES: ANGELINA, HOUSTON, JASPER, NACOGDOCHES, NEWTON, POLK, SABINE, SAN AUGUSTINE, SAN JACINTO, SHELBY, TRINITY, AND TYLER. THE NCTSI PROGRAM SHALL SERVE 120 INDIVIDUALS EACH YEAR, WITH A CUMULATIVE 500 UNDUPLICATED INDIVIDUALS SERVED OVER THE PROJECT PERIOD. THROUGH USE OF THESE FUNDS, BURKE SHALL EXPAND CURRENT SERVICE DELIVERY CAPACITY THROUGH EVIDENCE-BASED, CLINICAL-INFORMED TREATMENT OF COMPLEX TRAUMA, AND ENHANCE CURRENT TRAINING SYSTEMS, POPULATION HEALTH OUTCOMES, AND OTHER QUALITY MANAGEMENT ACTIVITIES TO IMPROVE QUALITY AND EFFECTIVENESS OF TRAUMA-INFORMED CARE BY ADDING 2.0 FTE THERAPISTS, 4.0 FTE MH CLINICIANS, 0.50 FTE TRAINER, AND 0.50 FTE PROJECT EVALUATOR. THESE STAFF SHALL REACH THE HISTORICALLY UNDER-SERVED TARGET POPULATION, REDUCE SPECIFIC SOCIAL DETERMINANTS OF HEALTH (SDOHS) RELATED TO ECONOMIC INSTABILITY AND ACCESS TO CARE, AND BUILD UPON CURRENT THERAPEUTIC CHILDREN AND ADOLESCENT-SPECIFIC PROJECTS. PROJECT STAFF WILL FOCUS ON MEASURABLE OBJECTIVES, INCLUDING INCREASING SCREENING FOR TRAUMA AT CENTRALIZED INTAKE BY 500 PER YEAR, REDUCE TRAUMA SYMPTOMOLOGY FOR CHILDREN WHO SCREEN POSITIVE BY 10%, IMPLEMENT OUTREACH EVENTS WITH COMMUNITY PARTNERS, COORDINATE WITH TEXAS CHRISTIAN UNIVERSITY’S KARYN PRUVIS INSTITUTE OF CHILD DEVELOPMENT, AND TRAIN 50 STAFF MEMBERS ANNUALLY ON TRAUMA-INFORMED INTERVENTIONS. BURKE SHALL FORMALLY PARTNER WITH EIGHT (8) ORGANIZATIONS WITHIN THE COMMUNITY, INCLUDING FOUR (4) SCHOOL DISTRICTS, TWO (2) SHERIFF’S OFFICES, ONE (1) CHILD WELFARE AGENCY, AND ONE (1) UNIVERSITY. THE NCTSI PROGRAM WILL EMPHASIZE REACHING HISTORICALLY UNDERSERVED POPULATIONS THROUGH UTILIZATION OF TELEHEALTH SERVICES FOR SUSTAINED ENGAGEMENT IN SERVICES. THE THERAPISTS WILL PROVIDE TRAUMA AND GRIEF COUNSELING TO THE POPULATION OF FOCUS, AND THE MH CLINICIANS WILL WORK WITH THE THERAPISTS TO LINK CONSUMERS WITH RECOVERY SUPPORT SYSTEMS ACROSS THE CONTINUUM OF CARE AND TO IMPROVE RETENTION, MAINTENANCE, AND DAILY FUNCTIONING POST-PROJECT. THE TRAINER SHALL PROVIDE EDUCATION TO BOTH GRANT-FUNDED STAFF AND BURKE PERSONNEL ON TRAUMA-INFORMED EVIDENCE-BASED PRACTICES. FINALLY, THE PROJECT EVALUATOR WILL ENDEAVOR TO MONITOR HEALTH OUTCOMES AND INEQUITY ACROSS THE PROJECT TIMELINE TO ADDRESS APPROPRIATE INTERVENTIONS WHEN NEEDED.

OPTICAL DISSECTION OF INTRACORTICAL CIRCUITS SUPPORTING MOTOR RECOVERY AFTER SPINAL CORD INJURY

COOPERATIVE AGREEMENT TO SUPPORT NAVIGATORS IN FEDERALLY-FACILITATED AND STATE PARTNERSHIP MARKETPLACES

TRANSCRIPTIONAL REGULATION OVER NEUROGENESIS OF CORTICAL OUTPUT NEURON SEGMENTAL IDENTITY AND DIVERSITY - SUBCEREBRAL PROJECTION NEURONS (SCPN) RESIDE IN THE NEOCORTEX, AND EXTEND AXONS TO SUBCEREBRAL TARGETS IN THE BRAINSTEM AND SPINAL CORD. CORTICOSPINAL NEURONS (CSN), A SUBCLASS OF SCPN, PROJECT TO THE SPINAL CORD AND THEIR AXONS FORM THE CORTICOSPINAL TRACT (CST), A CRITICAL CIRCUIT FOR VOLUNTARY MOTOR CONTROL. IN ADDITION, PROJECTIONS FROM THE NEOCORTEX TO BRAINSTEM TARGETS FUNCTION IN PARALLEL WITH CSN TO EXERT MOTOR CONTROL. DEGENERATION OF THESE PROJECTION NEURONS IN AMYOTROPHIC LATERAL SCLEROSIS (ALS), ALONG WITH DEGENERATION OF SPINAL MOTOR NEURONS, CAUSES SPASTICITY AND PARALYSIS. IN HUMANS, DAMAGE TO THE CST AFTER SPINAL CORD INJURY IS A PRINCIPAL CAUSE OF LOSS OF VOLUNTARY MOTOR CONTROL. FURTHER, INTEGRITY OF CORTICOSPINAL CONNECTIVITY IS CENTRALLY LINKED TO RECOVERY FROM STROKE AND CEREBRAL PALSY. THERE HAVE BEEN MULTIPLE INVESTIGATIONS DETAILING THE ROLE OF CORTICO-BRAINSTEM VS. CORTICOSPINAL PROJECTIONS IN BOTH MOTOR CONTROL, AS WELL AS THEIR DISTINCT CONTRIBUTIONS TO FUNCTIONAL RECOVERY IN THESE DISTINCT CAUSES OF PARALYSIS. HOWEVER, IT REMAINS UNCLEAR WHEN AND HOW THESE DISTINCT PROJECTIONS ARE ESTABLISHED DURING DEVELOPMENT. UNDERSTANDING THE MOLECULAR BASIS OF THIS SPECIFICATION AND DIFFERENTIATION DURING DEVELOPMENT THEREFORE HOLDS SIGNIFICANT PROMISE IN ESTABLISHING APPROACHES THAT ARE TAILORED TO ENHANCING PLASTICITY OF THESE RELATED, YET DISTINCT CIRCUITS. A NECESSARY FIRST STEP TOWARD THIS ULTIMATE GOAL IS TO IDENTIFY THE MOLECULAR MECHANISMS DIRECTING SCPN AXONS TO BRAINSTEM (CORTICO-BRAINSTEM NEURONS) VERSUS SPINAL CORD (CSN). ONGOING WORK IN OUR LAB HAS IDENTIFIED THAT SUCH PROJECTIONS ARE INITIALLY SPECIFIED DURING THE PROCESS OF AXON EXTENSION DURING DEVELOPMENT. WE HAVE IDENTIFIED THAT CORTICO-BRAINSTEM AND CORTICOSPINAL NEURONS EXPRESS DISTINCT GENES AND CAN BE MOLECULARLY DISTINGUISHED IN MICE BY BIRTH. FURTHER OUR DATA SUGGEST THAT THE TRANSCRIPTIONAL REGULATOR SATB2 ACTS, IN PART, TO SPECIFY CORTICO-BRAINSTEM NEURONS. THIS PROPOSAL INVESTIGATES THE HYPOTHESIS THAT TRANSCRIPTIONAL REGULATION CONTROLS THE DEVELOPMENT OF CORTICO-BRAINSTEM VS. CORTICOSPINAL PROJECTIONS BY LATE EMBRYONIC DEVELOPMENT IN MICE. BUILDING ON THIS FOUNDATION, WE WILL FIRST IDENTIFY THE TIME POINT IN DEVELOPMENT WHEN THESE PROJECTIONS ARE SPECIFIED USING KNOCK-IN CRE REPORTER MICE (AIM 1). THIS WILL BE TESTED USING INTRACEREBRAL INJECTIONS OF AAV-REPORTERS AT DISTINCT DEVELOPMENTAL TIMES IN UTERO WITH ADULT ANALYSIS OF AXONAL PROJECTIONS. IN AIM 2, WE WILL INVESTIGATE THE TRANSCRIPTIONAL TARGETS OF SATB2 IN SCPN AT LATER DEVELOPMENTAL TIMES BY PROFILING ALL SCPN IN SATB2 WT AND CONDITIONAL KO MICE, AS WELL AS BY SATB2 OVEREXPRESSION, AT SINGLE CELL RESOLUTION. FINALLY, IN AIM 3 USING SUBPOPULATION-SPECIFIC TRANSGENIC CRE LINES, WE WILL INVESTIGATE SCPN AXON TARGETING IN BOTH SATB2 LOSS- AND GAIN-OF-FUNCTION. IN ADDITION, WE WILL INVESTIGATE WHETHER MISEXPRESSION OF SATB2 TARGET GENES CAN ALTER SCPN TARGETING TO THE BRAINSTEM VS. SPINAL TARGETS. TOGETHER, OUR WORK WILL DISCERN IN-DEPTH, THE MECHANISMS OF WHEN AND HOW TRANSCRIPTIONAL REGULATION CONTROLS SCPN SEGMENTAL “IDENTITY” THEREBY PROVIDING A MECHANISTIC FRAMEWORK FOR SUBSEQUENT IDENTIFICATION OF MOLECULES CONTROLLING SEGMENTALLY APPROPRIATE SCPN CONNECTIVITY WITH SUBCEREBRAL TARGETS.

ROLE OF CD36 IN FUNCTIONAL RECOVERY IN CHRONIC STROKE

USING TRANSCRIPTION FACTORS TO ENHANCE TRANSPLANTED CELL SURVIVAL FOR SCI REPAIR

BURKE CCBHC EXPANSION GRANT

TARGETING STROKE-INDUCED BRAIN SWELLING IN OBESE SUBJECTS: ROLE OF VEGF

IMPACT OF SENSORY IMPAIRMENTS ON MOVEMENT IN CHILDREN WITH CEREBRAL PALSY

IMPACT OF BDNF SNP ON STROKE-INDUCED PLASTICITY AND MOTOR FUNCTION

ALLELIC CHOICE IN RETT SYNDROME

HDAC6: A TARGET FOR REGENERATION FOLLOWING INJURY IN THE NERVOUS SYSTEM

A NOVEL COMBINATORIAL APPROACH TO RESTORE MOTOR FUNCTION AFTER SPINAL CORD INJURY

MOTOR CORTEX ELECTRICAL STIMULATION TO AUGMENT SPONTANEOUS RECOVERY AFTER CHRONIC SUBCORTICAL STROKE

DOMESTIC WATER GRANTS - 09/10 STIMULUS

BENFOTIAMINE IN ALZHEIMER'S DISEASE: A PILOT STUDY

STUDYING THE MOLECULAR REGULATION OF MGE PROJECTION NEURON IDENTITY BY ST18 - PROJECT SUMMARY: THE MEDIAL GANGLIONIC EMINENCE (MGE) IS A NEURAL PROGENITOR DOMAIN LOCATED IN THE VENTRAL TELENCEPHALON. IT IS WELL-STUDIED FOR ITS PRODUCTION OF CORTICAL INTERNEURONS, A BROADLY DISTRIBUTED POPULATION OF LOCALLY-PRO- JECTING NEURONS THAT REGULATE CORTICAL RHYTHMICITY. THE MGE ALSO PRODUCES PROJECTION NEURONS THAT REMAIN IN THE VENTRAL FOREBRAIN, ARRANGED IN DISCRETE NUCLEI THAT PROJECT DISTALLY POINT-TO-POINT. INTERNEURON AND PROJECTION NEURON IDENTITY REPRESENT TWO FUNDAMENTALLY-DISTINCT POPULATIONS, WITH DISTINCT FUNCTIONS. HOW THE MGE DELINEATES BETWEEN THE PRODUCTION OF IN AND PN, HOWEVER, IS NOT UNDERSTOOD. WE HAVE IDENTIFIED A TRANSCRIPTION FACTOR, ST18 THAT APPEARS TO REGULATE THIS FUNDAMENTAL DELINEATION. WE PREVIOUSLY DESCRIBED A MODEL SYSTEM TO STUDY MGE DEVELOPMENT USING INDUCED EXPRESSION OF CANDIDATE FACTORS. WHEN ST18 WAS INTRO- DUCED IN THIS SYSTEM, THE CELLS LARGELY ADOPTED A PROJECTION NEURON IDENTITY. FURTHER, WHEN THESE CELLS WERE TRANSPLANTED IN UTERO, THEY REMAINED IN THE VENTRAL FOREBRAIN AND ADOPTED LONG-RANGE AXONAL PROJECTIONS REMINISCENT OF GLOBUS PALLIDUS PARS EXTERNA (GPE). MOREOVER, IN OUR INITIAL ANALYSIS OF THE ST18 CONDITIONAL LOSS-OF-FUNCTION MUTANT MOUSE, WE OBSERVED A SPE- CIFIC LOSS OF GPE NEURONS, WHILE ALL OTHER MGE LINEAGES APPEAR SPARED. IN THIS PROPOSAL, WE WILL; AIM 1) DETERMINE WHETHER ST18 INDUCED EXPRESSION IS SUFFICIENT TO DIRECT MGE PROGENITORS TOWARDS PROJECTION NEURON IDENTITY (TECHNIQUES: ESC DIRECTED DIFFERENTIATION, NEURONAL CULTURE, IN VITRO MIGRATION ASSAYS, UBM TRANSPLANTATION, RNA-SEQ). AIM 2) TEST WHETHER ST18 IS NECESSARY FOR MGE PROJECTION NEURON FATE BY ANALYSIS OF ST18 CONDITIONAL LOSS-OF-FUNCTION MOUSE (TECHNIQUES: MOUSE GENETICS, VIRAL AND DYE TRACING OF AXON PROJECTIONS, SCRNA- SEQ). AIM 3) MANIPULATE ST18 DURING MGE PROGENITOR DIFFERENTIATION TO PROBE THE TIME WINDOW OF ITS ACTION AND TEST HOW IT REGULATES GENE EXPRESSION IN DIRECTING PROJECTION NEURON IDENTITY (TECHNIQUES: ESC DIRECTED DIFFERENTIATION, IN VITRO MOR- PHOLOGY AND MIGRATION ASSAYS, CHIP-SEQ). IMPORTANTLY, IN THE COURSE OF OUR STUDY, WE WILL PERFORM A MULTI-LEVEL EXPRESSION AND CHIP-SEQ ANALYSIS TO UNCOVER THE GENE NETWORKS AND CELLULAR PATHWAYS UNDER THE REGULATION OF ST18 THAT COLLECTIVELY GOVERN ASPECTS OF PROJECTION NEURON IDENTITY: NEURONAL MIGRATION, POLARITY, PROCESS MORPHOGENESIS. IN SUMMARY, WE ARE UNIQUELY POSITIONED TO ADDRESS HOW MGE PROGENITORS REGULATE PROJECTION NEURON VERSUS INTERNEURON IDENTITY IN THE MGE LINEAGE. THROUGH THESE EFFORTS, WE AIM TO INFORM MORE BROADLY AS TO HOW PROJECTION AND INTERNEURON IDENTITY IS ASSIGNED THROUGHOUT THE NERVOUS SYSTEM.

MOLECULAR REGULATION OVER THE DECLINE IN LONG-DISTANCE CORTICOSPINAL AXON REGENERATIVE ABILITY DURING DEVELOPMENT - CORTICOSPINAL NEURONS (CSN) RESIDE IN THE NEOCORTEX, AND EXTEND AXONS TO SPECIFIC SEGMENTAL TARGETS IN THE SPINAL CORD FORMING THE CORTICOSPINAL TRACT (CST). CSN CRITICALLY CONTROL VOLUNTARY MOVEMENT AND ARE CENTRALLY INVOLVED IN RECOVERY FROM PARALYSIS ORIGINATING FROM MULTIPLE CAUSES, E.G., STROKE, SPINAL CORD INJURY (SCI), CEREBRAL PALSY, ETC.. CSN DEGENERATION IN AMYOTROPHIC LATERAL SCLEROSIS (ALS), ALONG WITH DEGENERATION OF SPINAL MOTOR NEURONS, CAUSES SPASTICITY AND PARALYSIS. RECOVERY IN ALL THESE DISTINCT CAUSES OF PARALYSIS WOULD ULTIMATELY REQUIRE LONG-DISTANCE CST REGENERATION, WHICH REMAINS AN UNATTAINED GOAL IN REGENERATIVE NEUROSCIENCE. PREVIOUS WORK USING NEONATAL LESIONS HAS ESTABLISHED CST REGENERATIVE ABILITY DECLINES FROM DEVELOPMENT INTO ADULTHOOD. WHEN THE CST IS DAMAGED IN EARLY LIFE, THERE IS GREATER PLASTICITY AND REGENERATION AS COMPARED TO SIMILAR LESIONS IN THE ADULT. HOWEVER, DESPITE THIS WORK, WE STILL DO NOT KNOW WHEN LONG-DISTANCE REGENERATIVE ABILITY IS LOST DURING DEVELOPMENT. THIS IS BECAUSE A KEY LIMITATION OF THESE ESTABLISHED NEONATAL LESION MODELS IS THAT THEY MASSIVELY DISRUPT THE SPINAL ENVIRONMENT AND THEREBY INTERFERE WITH THE NORMAL PROCESS OF LONG- DISTANCE CSN AXON EXTENSION DURING DEVELOPMENT. THIS LIMITS THE ABILITY OF THESE LESION PARADIGMS TO ASSESS THE ABILITY OF THE CNS TO SUPPORT LONG-DISTANCE REGENERATION. WE RECENTLY ESTABLISHED A NOVEL MICROSURGICAL APPROACH TO AXOTOMIZE THE CST DURING DEVELOPMENT, WHILE LEAVING THE SPINAL ENVIRONMENT RELATIVELY INTACT. WE IDENTIFIED THAT LONG-DISTANCE CST REGENERATIVE ABILITY IS LOST AT DIFFERENT TIMES AT DISTINCT SPINAL LEVELS– AT POSTNATAL DAY 4 (P4) THE CST CAN FULLY REGENERATE WHEN LESIONED AT THORACIC T11, BUT FAILS TO DO SO WHEN LESIONED AT CERVICAL C2. OUR RESULTS INDICATE THAT THE LOSS OF LONG-DISTANCE CST REGENERATIVE ABILITY CLOSELY PARALLELS THE DEVELOPMENTAL TIMELINE OF NORMAL CST GROWTH INTO THE SPINAL CORD, WHICH SUGGESTS THAT THE NORMAL PROCESS OF DIFFERENTIATION, BOTH IN CSN AND IN THE SPINAL CORD, RESULTS IN THE LOSS OF LONG-DISTANCE REGENERATIVE ABILITY. THIS PROPOSAL INVESTIGATES THE HYPOTHESIS THAT INHIBITION OF DIFFERENTIATION TO PROLONG THE IMMATURE DEVELOPMENTAL STATE WILL EXTEND THE TIME WINDOW WHEN LONG-DISTANCE REGENERATION IS POSSIBLE. SPECIFICALLY, WE WILL MANIPULATE THE FUNCTION OF RE1 SILENCING TRANSCRIPTION FACTOR (REST), A GLOBAL REPRESSOR OF NEURAL DIFFERENTIATION, TO TEST THIS HYPOTHESIS. BUILDING ON THIS FOUNDATION, WE WILL FIRST MANIPULATE REST FUNCTION (BOTH GAIN- AND LOSS-OF FUNCTION) AT DISTINCT SPINAL LEVELS TO INVESTIGATE WHETHER THIS AFFECTS THE ABILITY OF THESE SPINAL SEGMENTS TO SUPPORT LONG-DISTANCE CST REGENERATION (AIM1). WE WILL MANIPULATE REST FUNCTION IN CSN TO SIMILARLY INVESTIGATE LONG-DISTANCE CST REGENERATION (AIM 2). FINALLY, WE WILL USE SINGLE CELL PROFILING TO INVESTIGATE POTENTIALLY DISTINCT MOLECULAR EFFECTS OF MICROLESIONS AT DISTINCT SPINAL LEVELS ON DISTINCT CSN SUBSETS DEPENDING ON THEIR DEVELOPMENTAL STATE (AIM 3). TOGETHER, OUR WORK WILL DISCERN NOVEL MOLECULAR MECHANISMS OF HOW LONG-DISTANCE CST REGENERATION IS REGULATED DURING DEVELOPMENT, THEREBY PROVIDING A MECHANISTIC FRAMEWORK FOR SUBSEQUENT IDENTIFICATION OF MOLECULES THAT CAN BE USED TO EFFECT LONG-DISTANCE CST REGENERATION IN THE ADULT CNS.

PPHF ? 2013 - COOPERATIVE AGREEMENT TO SUPPORT NAVIGATORS IN FEDERALLY-FACILITATED AND STATE PARTNERSHIP EXCHANGES

INJURY AND ADAPTATION IN THE DEVELOPING RAT CORTICOSPINAL AND RUBROSPINAL TRACTS

AXONAL TRANSPORT AND LOCAL TRANSLATION IN NEUROPATHIC PAIN

DOMESTIC WATER GRANTS - 09/10 STIMULUS

THE PURPOSE OF THE COPS HIRING PROGRAM (CHP) PROGRAM IS TO ADVANCE THE PRACTICE OF COMMUNITY POLICING THROUGH THE HIRE OR REHIRE OF ADDITIONAL CAREER LAW ENFORCEMENT OFFICERS. FUNDING UNDER THIS AWARD PROGRAM WILL BE UTILIZED BY LOCAL LAW ENFORCEMENT AGENCIES TO HIRE AND REHIRE CAREER LAW ENFORCEMENT OFFICERS NECESSARY TO INCREASE THE JURISDICTIONS COMMUNITY POLICING CAPACITY TO PREVENT AND DISRUPT CRIME AND VIOLENCE.

CROSS-TALK BETWEEN SARMOPTOSIS AND FERROPTOSIS IN HEMORRHAGIC STROKE - PROJECT SUMMARY/ABSTRACT BRAIN BLEEDING IN THE PARENCHYMA, ALSO KNOWN AS INTRACEREBRAL HEMORRHAGE (ICH), REPRESENTS 10 TO 30 PERCENT OF STROKES IN DISTINCT COUNTRIES AROUND THE WORLD. DESPITE ITS LOWER INCIDENCE THAN ISCHEMIC STROKE, ICH CARRIES HIGHER RATES OF MORTALITY AND MORBIDITY. WORSE OUTCOMES IN HUMANS WITH ICH HAVE BEEN CORRELATED WITH DAMAGE TO AXONS IN AREAS OF THE BRAIN LIKE THE THALAMUS OR THE STRIATUM WHERE BLEEDING FROM SMALL, PENETRATING ARTERIES (OFTEN AFFECTED BY LONGSTANDING HYPERTENSION) TENDS TO OCCUR. RED BLOOD CELLS ACCUMULATE IN ICH AND LYSE AND RELEASE TOXINS. THESE INCLUDE HEMOGLOBIN (HGB) AND ITS BREAKDOWN PRODUCT HEMIN . ADDITIONAL UNDERSTANDING OF HOW HGB/HEMIN LEADS AXONS TO DEGENERATE IN ICH IS A STRATEGY TO FULLY ASSESS AND OVERCOME DISABILITY FROM THIS CONDITION. EXCITINGLY, A MOLECULAR PATH FOR PROGRAMMED AXONAL DESTRUCTION HAS BEEN DEFINED INVOLVING THE PROTEIN, STERILE ALPHA AND TOLL/INTERLEUKIN RECEPTOR-1 (TIR) MOTIF CONTAINING 1 (SARM1). SARM1'S DEGENERATIVE ACTIVITIES IN THE AXON EMERGE FROM ITS NADASE (NAD+ DEGRADING) ACTIVITY IN THE TIR DOMAIN. THIS DOMAIN'S IMPORTANCE IS HIGHLIGHTED BY ITS CONSERVATION FROM PLANTS TO HUMANS. IN PRELIMINARY STUDIES, WE FOUND THAT GERMLINE DELETION OF FULL LENGTH SARM1 PREVENTS AXONAL DAMAGE IN THE STRIATUM FOLLOWING ICH. SINCE PREVIOUS STUDIES FROM OUR LAB HAVE SHOWN THAT HEMIN INDUCES FERROPTOSIS, AN IRON-DEPENDENT, CASPASE-INDEPENDENT FORM OF PROGRAMMED NECROSIS, FOLLOWING EXPERIMENTAL ICH, WE ARE SEEKING TO UNDERSTAND THE CROSS TALK BETWEEN FERROPTOSIS AND SARM1-MEDIATED AXONAL DESTRUCTION (SARMOPTOSIS). OF NOTE, WE FOUND THAT CORTICAL NEURONS CULTURED FROM THE SARM1 KNOCKOUT MICE ARE PARTIALLY RESISTANT TO FERROPTOTIC STIMULI (HEMIN AND ERASTIN). MOREOVER, AGENTS SUCH AS P7C3 THAT AUGMENT THE CELL'S ABILITY TO MAKE NAD+ PROTECT AGAINST FERROPTOSIS; CONVERSELY, FK866, WHICH IMPEDES THE SAME NAD+ SYNTHESIZING PATHWAY MAKES FERROPTOSIS WORSE. FROM THESE PRELIMINARY STUDIES WE PROPOSE THREE AIMS DESIGNED TO UNDERSTAND THE INTERPLAY BETWEEN FERROPTOSIS AND SARMOPTOSIS IN ICH. IN THE FIRST AIM, WE WILL LEVERAGE MICE THAT POSSESS A GERMLINE, HOMOZYGOUS, SINGLE AMINO ACID MUTATION (E642A) IN THE NADASE DOMAIN OF SARM1. LIKE THE SARM1-/- MICE, THE NEURONS OF THESE MICE ARE RESISTANT TO AXOTOMY IN VITRO AND IN VIVO. THEY WILL BE USED TO PROBE WHETHER THE NADASE ACTIVITY IS CRITICAL FOR FERROPTOSIS IN VITRO AND ICH IN VIVO. IN THE SECOND AIM, WE WILL ADDRESS THE HYPOTHESIS THAT HEMIN-INDUCES AXONAL DEGENERATION VIA LOCAL FERROPTOTIC PATHWAYS THAT TRIGGER SARM1 ACTIVATION. WE WILL USE SENSORY NEURONS CULTURED IN CAMPENOT CHAMBERS TO ISOLATE CELL BODIES FROM AXONS AND QUERY THEIR POSSIBLY DISTINCT RESPONSES TO LETHAL CONCENTRATIONS OF HEMIN. WE WILL ALSO PROBE THE ROLE OF FERROPTOSIS AND SARMOPTOSIS IN THE DESTRUCTION OF CORTICOSPINAL TRACT AXONS IN VIVO. IN THE LAST AIM, WE WILL SEEK TO UNDERSTAND WHETHER INTERVENTIONS THAT TARGET FERROPTOSIS BY ACTING IN THE NUCLEUS (CELL BODY) CAN BE COMBINED WITH SARM1 DELETION (THAT ACTS PRIMARILY IN AXONS) IN MICE TO REDUCE DAMAGE AND IMPROVE BEHAVIORAL RECOVERY OVER EACH AGENT TESTED ALONE. AT THE CULMINATION OF THESE STUDIES, NEW MECHANISTIC INFORMATION WILL EMERGE REGARDING AXONAL AND CELL BODY LOSS FOLLOWING ICH WITH CLEAR THERAPEUTIC IMPLICATIONS.

CHP

PUBLIC HOUSING CAPITAL FUND

BURKE SUBSTANCE ABUSE NETWORK COMMUNITY INITIATIVE TO ADDRESS SUBSTANCE USE/MISUSE IN BURKE COUNTY, NC WITH A FOCUS ON ALCOHOL AND NON-MEDICAL USE OF PRESCRIPTION DRUGS

HARBOUR-LEAD PROJECT

AUTOMATED ASSESSMENT OF VISUOMOTOR FUNCTION IN CHILDREN WITH BRAIN INJURY

ADVANCED, MULTI-FUNCTION, INVERTED CONFOCAL MICROSCOPE FOR BNI STRUCTURAL AND FUNCTIONAL IMAGING CORE

NON-INVASIVE STIMULATION FOR IMPROVING MOTOR FUNCTION IN SPINAL CORD INJURY

BURKE SUBSTANCE ABUSE NETWORK COMMUNITY INITIATIVE TO ADDRESS SUBSTANCE USE/MISUSE IN BURKE COUNTY, NC WITH A FOCUS ON ALCOHOL AND NON-MEDICAL USE OF PRESCRIPTION DRUGS

BURKE COUNTY RECOVERY COURT IMPLEMENTATION

ADVANCED MATERIALS FOR SAFE AND EFFECTIVE STIMULATION OF THE RAT CERVICAL SPINAL CORD

COOPERATIVE AGREEMENT TO SUPPORT NAVIGATORS IN FEDERALLY-FACILITATED AND STATE PARTNERSHIP MARKETPLACES

USING NATURAL MOUSE MOVEMENT TO ESTABLISH A DEVELOPMENTAL "BIOMARKER" FOR CORTICOSPINAL DAMAGE - THE CORTICOSPINAL TRACT (CST) IS A CRITICAL CIRCUIT UNDERLYING SKILLED VOLUNTARY MOVEMENTS. DAMAGE TO THIS CIRCUIT DURING DEVELOPMENT CAN CAUSE PERMANENT, LONG-TERM MOVEMENT DISABILITY IN HUMANS. RECOGNIZING AND TREATING SUCH DEVELOPMENTAL CST DAMAGE IS CHALLENGING, LARGELY BECAUSE IMMEDIATELY AFTER THE LESION, THERE ARE LIMITED OR ALMOST NO FUNCTIONAL DEFICITS. HOWEVER, EARLY RECOGNITION AND INTERVENTION WITH THE APPROPRIATE TREATMENT MEASURES IS KEY TO REDUCING LONG-TERM DISABILITY. THE USE OF PRECLINICAL MOUSE MODELS, WHICH HAVE OTHERWISE PROVEN TO BE HIGHLY USEFUL IN FUNCTIONAL INVESTIGATIONS OF NERVOUS SYSTEM DEVELOPMENT, HAS BEEN LIMITED IN THIS REGARD. SINCE THE CST IS KNOWN TO CONTROL SKILLED MOVEMENTS, ESTABLISHED BEHAVIORAL TESTS IN MICE THAT INVESTIGATE CST FUNCTION REQUIRE TRAINING MICE IN SKILLED TASKS. THIS PRECLUDES THEIR APPLICATION IN NEONATAL MICE. FURTHER, MOUSE MODELS USED TO INVESTIGATE DEVELOPMENTAL CST DAMAGE, E.G. NEONATAL HYPOXIA OR SPINAL INJURIES, DO NOT ONLY DAMAGE THE CST; RATHER THEY DISRUPT MULTIPLE NEURAL PATHWAYS. IT THEREFORE REMAINS COMPLETELY UNKNOWN WHETHER THE CST CONTRIBUTES ONLY TO SKILLED MOVEMENTS IN ADULT MICE, OR WHETHER IT ALSO CONTRIBUTES TO THE DEVELOPMENT OF NATURAL, INNATE MOTOR ABILITY DURING DEVELOPMENT, BEGINNING IN NEONATAL MICE. THIS LATTER POSSIBILITY WOULD SUGGEST THAT THERE ARE EARLY, ALBEIT SUBTLE, BEHAVIORAL CORRELATES OF DEVELOPMENTAL CST INJURY IN MICE. WE RECENTLY DEVELOPED A NEW MICROSURGICAL APPROACH TO SPECIFICALLY DISRUPT THE DEVELOPING CST IN NEONATAL MICE. IN ADDITION, WE HAVE ALSO ESTABLISHED THE USE OF MOTION SEQUENCING (MOSEQ), A NEW MACHINE LEARNING AND ARTIFICIAL INTELLIGENCE PLATFORM, TO LONGITUDINALLY INVESTIGATE THE DEVELOPMENT OF NATURAL MOVEMENTS IN NEONATAL MICE. OUR PRELIMINARY RESULTS USING MOSEQ SUGGEST THAT DEVELOPMENTAL DAMAGE TO THE CST RESULTS IN SPECIFIC CHANGES TO MOVEMENT STRUCTURES IN MICE, AS EARLY AS P12; THESE EXTEND INTO MATURITY AT P35. FURTHER, ANALYSIS OF THESE P35 MICE USING CONVENTIONAL METRICS OF LOCOMOTION SUCH AS THE CATWALK, DID NOT IDENTIFY ANY DEFICITS, HIGHLIGHTING THE SENSITIVITY OF MOSEQ IN IDENTIFYING CHANGES IN MOUSE MOVEMENTS. THIS PROPOSAL INVESTIGATES THE HYPOTHESIS THAT THE CST CONTROLS DEVELOPMENT OF NATURAL MOUSE MOVEMENTS, AND NOT ONLY SKILLED MOVEMENTS AT MATURITY. WE WILL USE MOSEQ TO ANALYZE FEZF2 KNOCK OUT (FEZF2 KO) MICE IN WHICH THE CST IS NEVER ESTABLISHED DURING DEVELOPMENT (AIM1), AS WELL AS MICE THAT UNDERGO MICROSURGICAL LESIONS TO DISRUPT SPINAL CONNECTIVITY OF THE CST AT DISTINCT DEVELOPMENTAL TIMES (AIM2). TOGETHER, OUR WORK WILL IDENTIFY NOVEL FUNCTIONAL READOUTS OF DEVELOPMENTAL DAMAGE TO THE CST USING NATURAL MOUSE MOVEMENTS. THIS NEW UNBIASED QUANTITATIVE APPROACH TOWARD INVESTIGATING THE EARLIEST BEHAVIORAL SIGNS OF CORTICOSPINAL DYSFUNCTION IN MICE WHICH WILL HAVE EVENTUAL APPLICATION IN INVESTIGATIONS OF DESCENDING CIRCUITS OF MOTOR CONTROL, AS WELL AS MULTIPLE PRECLINICAL MODELS OF DEVELOPMENTAL DAMAGE SUCH AS NEONATAL HYPOXIA OR SPINAL INJURIES.

LOSS OF A COHORT OF AXONAL MRNAS AS A RESULT OF REDUCED SMN LEVELS.

DISSECTING MOTOR CORTEX MODULATION OF NOCICEPTION DURING CHRONIC PAIN - PROJECT SUMMARY (SEE INSTRUCTIONS): THE HEAVY BURDEN OF CHRONIC PAIN AND THE OPIOID EPIDEMIC HAS PROMPTED AN URGENT, WORLDWIDE SEARCH FOR ALTERNATIVE, NON-ADDICTIVE METHODS OF ANALGESIA. ONE PROMISING ALTERNATIVE IS NON-INVASIVE ELECTRICAL OR MAGNETIC STIMULATION OF THE MOTOR CORTEX (MC). WHILE MC STIMULATION (MCS) HAS REPEATEDLY BEEN FOUND TO REDUCE CHRONIC PAIN IN HUMAN SUBJECTS AND DECREASE NOCICEPTIVE BEHAVIORS IN RODENT MODELS, MAJOR QUESTIONS REMAIN ABOUT ITS MECHANISM OF ACTION AND HOW TO IMPROVE MCS EFFICACY. EVIDENCE SUGGESTS THAT MCS ANTINOCICEPTIVE EFFICACY INCREASES WHEN THE STIMULATION TARGETS THE REGION IN MOTOR CORTEX THAT CORRESPONDS TO THE BODY PART FROM WHICH PAIN ORIGINATES (SOMATOTOPICALLY MATCHING MCS) AND THAT MCS ANALGESIA REQUIRES ENDOGENOUS OPIOID ACTIVITY. HERE, I PROPOSE TO USE A RODENT MODEL OF TRIGEMINAL NEUROPATHIC PAIN TO ELUCIDATE THE UNDERLYING MECHANISM OF MCS ANTINOCICEPTION AND TO DEFINE KEY MCS FEATURES THAT PAIN CLINICIANS CAN USE TO IMPROVE MCS EFFICACY. TO CHARACTERIZE THE MCS MECHANISM, I WILL 1) QUANTIFY THE EFFICACY OF SOMATOTOPICALLY MATCHED MCS (SSMCS), 2) DETERMINE WHAT OPIOID RECEPTOR SUBTYPES ARE REQUIRED FOR MCS ANTINOCICEPTION, AND 3) IDENTIFY HOW ENDOGENOUS OPIOIDS MODULATE AN OPIOID-SENSITIVE MC DESCENDING CIRCUIT TO THE DESCENDING PAIN CONTROL PATHWAYS DURING SSMCS. TO DETERMINE THE EFFICACY OF MCS BETWEEN MATCHED AND OFF-TARGET MCS IN TWO DIFFERENT NERVE CONSTRICTION MODELS, I WILL USE CLASSIC BEHAVIORAL PARADIGMS ALONG WITH CUTTING-EDGE MACHINE LEARNING ALGORITHMS TO ANALYZE MOUSE BEHAVIORAL RESPONSES. I WILL THEN USE COMPLEMENTARY PHARMACOLOGICAL AND GENETIC MOUSE LINES TO DETERMINE HOW SSMCS IS IMPACTED BY DISTINCT OPIOID RECEPTOR TYPES. TO INTERROGATE THE COMBINED IMPACT OF MC SOMATOTOPY AND ENDOGENOUS OPIOID SIGNALING IN MCS ANALGESIA, I WILL FOCUS ON THE DESCENDING PROJECTION FROM MC TO DESCENDING PAIN CONTROL REGIONS, ROSTRAL VENTROMEDIAL MEDULLA (RVM) AND SPINAL TRIGEMINAL NUCLEUS CAUDALIS (SPVC). I WILL DETERMINE THE POSITIONS OF OPIOID RECEPTOR TYPES AND ENDOGENOUS OPIOID PEPTIDES ALONG THIS CIRCUIT AND THEN ASSESS THE IMPACT OF SSMCS WITH AND WITHOUT OPIOID SIGNALING ON NEURAL ACTIVITY IN MC, RVM, AND SPVC USING CUTTING-EDGE HIGH-DENSITY ELECTROPHYSIOLOGICAL TECHNIQUES. ALTOGETHER, THIS PROJECT WILL DETERMINE HOW MCS MODULATES NOCICEPTION THROUGH ENDOGENOUS OPIOID SIGNALING IN SOMATOTOPICALLY ALIGNED CIRCUITS. THE RESULTS WILL PROVIDE THE FIRST REPORT OF NEURAL ACTIVITY DURING AND AFTER SSMCS AT BOTH THE TARGET AND IN AN MC OUTPUT.

THE PURPOSE OF THE COPS OFFICE SCHOOL VIOLENCE PREVENTION PROGRAM (SVPP) IS TO IMPROVE SECURITY AT SCHOOLS AND ON SCHOOL GROUNDS THROUGH SCHOOL SAFETY PROGRAMS AND TECHNOLOGY. FUNDING UNDER THIS AWARD PROGRAM WILL BE USED BY THE SCHOOL JURISDICTION TO IMPLEMENT EVIDENCE-BASED PROGRAMS TO IMPROVE SCHOOL SECURITY.

TRANSCRANIAL DIRECT CURRENT STIMULATION AND MOTOR TRAINING IN CHRONIC STROKE

PURPOSE: THE PUBLIC HOUSING CAPITAL FUND PROGRAM (CFP) WAS CREATED BY AN AMENDMENT TO THE 1937 ACT BY THE QUALITY HOUSING AND WORK RESPONSIBILITY ACT (QHWRA) IN 1998 (ADDING SECTION 9(D) TO THE 1937 ACT MERGING PREVIOUS MODERNIZATION AND DEVELOPMENT PROGRAMS). THE CFP PROVIDES FINANCIAL ASSISTANCE IN THE FORM OF GRANTS TO APPROXIMATELY 2,770 PUBLIC HOUSING AGENCIES (PHAS), SERVING NEARLY ONE MILLION UNITS, IN ALL 50 STATES AND TERRITORIES, TO CARRY OUT CAPITAL AND MANAGEMENT ACTIVITIES INCLUDING THOSE LISTED IN SECTION 9(D)(1) OF THE UNITED STATES HOUSING ACT OF 1937 (1937 ACT). THE MAIN PURPOSE OF THE CFP FORMULA GRANT IS TO FUND PUBLIC HOUSING MODERNIZATION, DEVELOPMENT, MANAGEMENT IMPROVEMENTS, AND THE OTHER ELIGIBLE ACTIVITIES DESCRIBED IN 24 CFR PART 905. ADDITIONAL INFORMATION ON THE PUBLIC HOUSING CAPITAL FUND IS LOCATED ON THE OFFICE OF CAPITAL IMPROVEMENTS WEBSITE: OFFICE OF CAPITAL IMPROVEMENTS | HUD.GOV / U.S. DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT (HUD) ADDITIONAL INFORMATION ON PUBLIC HOUSING FUNDING CAN BE FOUND BY ACCESSING THE WEBSITE BELOW AND REVIEWING THE PUBLIC HOUSING DASHBOARD LINKED UNDER THE “DATA DASHBOARD AND ANALYTICS”. PUBLIC HOUSING | HUD.GOV / U.S. DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT (HUD); ACTIVITIES TO BE PERFORMED: THE PHAS RECEIVE FEDERAL FUNDS FROM THE U.S. DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT (HUD) TO ADMINISTER THE PUBLIC HOUSING FUND. PUBLIC HOUSING CAPITAL FUNDS MAY ONLY BE USED FOR ACTIVITIES THAT ARE DESCRIBED AS ELIGIBLE ACTIVITIES IN 24 CFR 905.200 AND ARE EITHER SPECIFIED IN AN APPROVED 5-YEAR ACTION PLAN OR APPROVED BY HUD FOR EMERGENCY WORK OR WORK NEEDED BECAUSE OF A NON-PRESIDENTIALLY DECLARED NATURAL DISASTER. PUBLIC HOUSING DEVELOPMENT, MODERNIZATION, AND FINANCING ARE THE MAJOR ACTIVITIES TO BE PERFORMED. DEVELOPMENT IS ACTIVITIES AND RELATED COSTS THAT ADD TO (OR SIGNIFICANTLY RECONFIGURE) PUBLIC HOUSING UNITS IN A PHA’S INVENTORY, INCLUDING CONSTRUCTION AND ACQUISITION OF ADDITIONAL PUBLIC HOUSING UNITS, WITH OR WITHOUT REHABILITATION, AND ANY-AND-ALL UNDERTAKINGS NECESSARY FOR PLANNING, DESIGN, FINANCING, LAND ACQUISITION, DEMOLITION, CONSTRUCTION, OR EQUIPMENT OF PUBLIC HOUSING UNITS, AND RELATED BUILDINGS, FACILITIES, AND/OR APPURTENANCES (I.E., NON-DWELLING FACILITIES/SPACES). DEVELOPMENT ALSO INCLUDES ANY MIXED-FINANCE MODERNIZATION, ALL RELEVANT MODERNIZATION USES (OTHER THAN MANAGEMENT IMPROVEMENTS), FINANCING USES, AND DEVELOPMENT OF NON-DWELLING SPACE WHERE SUCH SPACE IS NEEDED TO ADMINISTER, AND IS OF DIRECT BENEFIT TO A PUBLIC HOUSING PROJECT (I.E. HOUSING DEVELOPED, ACQUIRED, OR ASSISTED BY A PHA UNDER THE 1937 ACT, AND THE IMPROVEMENT OF ANY SUCH HOUSING), INCLUDING THE RESIDENTS. FINANCING DEBT AND FINANCING COSTS (E.G., ORIGINATION FEES, INTEREST) INCURRED BY A PHA FOR DEVELOPMENT OR MODERNIZATION OF PUBLIC HOUSING PROJECTS, INCLUDING MIXED-FINANCE DEVELOPMENT, THE CAPITAL FUND FINANCING PROGRAM (CFFP), AND ANY OTHER USE AUTHORIZED UNDER SECTION 30 OF THE 1937 ACT. MODERNIZATION INCLUDES ALL ELIGIBLE ACTIVITIES EXCEPT FOR DEVELOPMENT AND FINANCING. PHYSICAL WORK IS A MAJOR ACTIVITY AND IS WORK THAT IS DONE ON THE PHYSICAL STRUCTURES, SITE, AND GROUNDS OF A PUBLIC HOUSING PROPERTY OR STRUCTURE. MAJOR PHYSICAL ACTIVITIES INCLUDE DEMOLITION, RECONFIGURATION, EMERGENCY ACTIVITIES, ENERGY EFFICIENCY, NON-ROUTINE MAINTENANCE, PLANNED CODE COMPLIANCE, AND VACANCY REDUCTION. THE MEASURABLE OUTCOME OF THIS GRANT IS THAT HUD WILL BE ABLE TO TRACK THE AMOUNT OF DOLLARS SPENT ON IMPROVEMENTS TO THE STRUCTURES, UNITS, COMMON AREAS, UTILITIES, AND OTHER ELIGIBLE ACTIVITIES. ; EXPECTED OUTCOMES: THE EXPECTED OUTCOMES FOR PUBLIC HOUSING CAPITAL FUNDS OF APPROXIMATELY $3.2 BILLION WILL BE PUT INTO THE DEVELOPMENT, MODERNIZATION, AND FINANCING OF NEARLY 1 MILLION PUBLIC HOUSING UNITS ACROSS ALL 50 STATES AND TERRITORIES. THE PUBLIC HOUSING UNITS ARE UPDATED TO BE DECENT, SAFE, SANITARY AND TO COMPLY WITH FEDERAL HOUSING STANDARDS. PHAS CAN ALSO USE A PORTION OF THE CAPITAL FUNDING FOR MANAGEMENT IMPROVEMENTS OR OPERATING ACTIVITIES INCLUDING SAFETY AND SECURITY COSTS.; INTENDED BENEFICIARIES: THE INTENDED BENEFICIARIES FOR PUBLIC HOUSING CAPITAL FUNDS ARE THE LOW-INCOME PUBLIC HOUSING RESIDENTS.; SUBRECIPIENT ACTIVITIES: THE RECIPIENT DOES NOT INTEND TO SUBAWARD FUNDS.

LEA GRANTS

ASSESSING NEURONAL VULNERABILITY IN THE ENTORHINAL CORTEX DUE TO ALZHEIMER'S DISEASE PATHOLOGY - PROJECT SUMMARY UNDERSTANDING HOW SPECIFIC NEURONS IN A SELECTIVELY VULNERABLE REGION SUCH AS THE EC ARE AFFECTED IS CRITICAL TO UNRAVELING THE MECHANISMS UNDERLYING ALZHEIMER'S DISEASE ONSET. WE AND OTHERS HAVE SHOWN THAT NEURONAL HYPEREXCITABILITY IS THE KEY DRIVER THAT MAKES PATHOLOGIES AND SYMPTOMS WORSE AND AMELIORATING HYPEREXCITABILITY SLOWS DOWN AD-RELATED SYMPTOMS. HOWEVER, IT IS UNCLEAR IF THE HYPEREXCITABILITY IS DUE TO AN INCREASED FIRING OF EXCITATORY NEURONS OR DECREASED FIRING OF INHIBITORY NEURONS. WE AIM TO UNDERSTAND THE MECHANISMS UNDERLYING HYPEREXCITABILITY BY STUDYING THE EXCITATORY AND INHIBITORY NEURONS IN THE EC OF APP AND TAU KNOCKIN MICE. TO DETERMINE WHICH TYPE OF NEURONS ARE AFFECTED EARLY IN THE EC, WE WILL INVESTIGATE: AIM 1- EXCITATORY NEURONS IN THE APP KNOCKIN (APPNL-G-F) AND TAU KNOCKIN (HTAU-KI) MICE BY USING INTERSECTIONAL APPROACH WITH OPTOGENETICS AND CHEMOGENETICS IN COMBINATION WITH ELECTROPHYSIOLOGY RECORDINGS AND BEHAVIOR AND AIM 2- INHIBITORY NEURONS (PARVALBUMIN- PV AND SOMATOSTATIN- SST) IN THE APPNL-G-F AND HTAU-KI MICE AS IN AIM 1. USING CRE-SPECIFIC MICE TO TARGET EXCITATORY AND INHIBITORY NEURONS IN COMBINATION WITH OPTO- OR CHEMO- GENETIC APPROACH WE WILL NOT ONLY IDENTIFY SELECTIVELY VULNERABLE NEURON-TYPE, BUT WE WILL ALSO MODULATE THEIR ACTIVITIES TO RESTORE THEIR FUNCTION AND TEST IF BEHAVIORAL DEFICITS ARE REVERSED. WE WILL USE NOVEL COMPUTATIONAL APPROACHES SUCH AS DECODING AND REMAPPING TO DETECT SUBTLE CHANGES IN FIRING ACTIVITY OF EC NEURONS. WE WILL ALSO ASSESS HOW AMYLOID BETA OR TAU AFFECT DIFFERENT NEURON TYPES IN THE EC, AND IF DOWNSTREAM HIPPOCAMPAL NEURONS ARE AFFECTED TOO. THE RESULTS OF THE PROPOSAL WILL DEFINITIVELY ANSWER IF HYPEREXCITABILITY OF NEURONS DUE TO AD PATHOLOGIES IS CAUSED BY DYSFUNCTION OF EXCITATORY OR INHIBITORY NEURONS. THE SENSITIVE COMPUTATIONAL METHODS USED IN THIS PROPOSAL ALLOW FOR MEASUREMENT OF SUBTLE ELECTROPHYSIOLOGICAL CHANGES NOT POSSIBLE PREVIOUSLY AND THEREFORE MIGHT ALLOW FOR DEVELOPMENT OF BETTER DIAGNOSTIC TESTS THAT COULD BE TRANSLATED AND MODIFIED FOR CLINICAL USE IN HUMANS. OVERALL, THE PROPOSAL AIMS TO IDENTIFY WHICH NEURONS IN THE MEC ARE MOST VULNERABLE TO AD PATHOLOGY. THE PROPOSAL IS HIGHLY INNOVATIVE AS IT WILL USE A COMBINATION OF SOPHISTICATED IN VIVO RECORDING IN TWO AD MOUSE MODELS IN COMBINATION WITH OPTOGENETIC AND CHEMOGENETIC TOOLS TO IDENTIFY SPECIFIC CELL TYPE AND MODULATE NEURONAL FIRING TO RESTORE FUNCTION. THE RESULTS FROM THE STUDY WILL BE HIGHLY SIGNIFICANT AS THERAPEUTIC OR CLINICAL APPROACHES TO TARGET VULNERABLE CELL TYPE COULD BE IDENTIFIED. THE PROPOSAL BRINGS TOGETHER DIVERSE FIELDS (ELECTROPHYSIOLOGY, PATHOLOGY AND COMPUTATIONAL NEUROSCIENCE) APPLYING LARGE-SCALE RECORDING TECHNIQUES TO RECORD ENSEMBLE POPULATIONS OF NEURONS TO INTERROGATE WHAT CAUSES HYPEREXCITABILITY IN A VULNERABLE BRAIN REGION THAT IS DYSFUNCTIONAL IN ALZHEIMER’S DISEASE.

FABRICATION AND INSTALLATION OF EXHIBITION INFRASTRUCTURE IN THE NEW BURKE MUSEUM

RURAL HEALTH NETWORK DEVELOPMENT PROGRAM

PERFORM FUNDING SYS

PUBLIC AND INDIAN HOUSING

PURPOSE: THE PUBLIC HOUSING CAPITAL FUND PROGRAM (CFP) WAS CREATED BY AN AMENDMENT TO THE 1937 ACT BY THE QUALITY HOUSING AND WORK RESPONSIBILITY ACT (QHWRA) IN 1998 (ADDING SECTION 9(D) TO THE 1937 ACT MERGING PREVIOUS MODERNIZATION AND DEVELOPMENT PROGRAMS). THE CFP PROVIDES FINANCIAL ASSISTANCE IN THE FORM OF GRANTS TO APPROXIMATELY 2,770 PUBLIC HOUSING AGENCIES (PHAS), SERVING NEARLY ONE MILLION UNITS, IN ALL 50 STATES AND TERRITORIES, TO CARRY OUT CAPITAL AND MANAGEMENT ACTIVITIES INCLUDING THOSE LISTED IN SECTION 9(D)(1) OF THE UNITED STATES HOUSING ACT OF 1937 (1937 ACT). THE MAIN PURPOSE OF THE CFP FORMULA GRANT IS TO FUND PUBLIC HOUSING MODERNIZATION, DEVELOPMENT, MANAGEMENT IMPROVEMENTS, AND THE OTHER ELIGIBLE ACTIVITIES DESCRIBED IN 24 CFR PART 905. ADDITIONAL INFORMATION ON THE PUBLIC HOUSING CAPITAL FUND IS LOCATED ON THE OFFICE OF CAPITAL IMPROVEMENTS WEBSITE: OFFICE OF CAPITAL IMPROVEMENTS | HUD.GOV / U.S. DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT (HUD) ADDITIONAL INFORMATION ON PUBLIC HOUSING FUNDING CAN BE FOUND BY ACCESSING THE WEBSITE BELOW AND REVIEWING THE PUBLIC HOUSING DASHBOARD LINKED UNDER THE “DATA DASHBOARD AND ANALYTICS”. PUBLIC HOUSING | HUD.GOV / U.S. DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT (HUD); ACTIVITIES TO BE PERFORMED: THE PHAS RECEIVE FEDERAL FUNDS FROM THE U.S. DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT (HUD) TO ADMINISTER THE PUBLIC HOUSING FUND. PUBLIC HOUSING CAPITAL FUNDS MAY ONLY BE USED FOR ACTIVITIES THAT ARE DESCRIBED AS ELIGIBLE ACTIVITIES IN 24 CFR 905.200 AND ARE EITHER SPECIFIED IN AN APPROVED 5-YEAR ACTION PLAN OR APPROVED BY HUD FOR EMERGENCY WORK OR WORK NEEDED BECAUSE OF A NON-PRESIDENTIALLY DECLARED NATURAL DISASTER. PUBLIC HOUSING DEVELOPMENT, MODERNIZATION, AND FINANCING ARE THE MAJOR ACTIVITIES TO BE PERFORMED. DEVELOPMENT IS ACTIVITIES AND RELATED COSTS THAT ADD TO (OR SIGNIFICANTLY RECONFIGURE) PUBLIC HOUSING UNITS IN A PHA’S INVENTORY, INCLUDING CONSTRUCTION AND ACQUISITION OF ADDITIONAL PUBLIC HOUSING UNITS, WITH OR WITHOUT REHABILITATION, AND ANY-AND-ALL UNDERTAKINGS NECESSARY FOR PLANNING, DESIGN, FINANCING, LAND ACQUISITION, DEMOLITION, CONSTRUCTION, OR EQUIPMENT OF PUBLIC HOUSING UNITS, AND RELATED BUILDINGS, FACILITIES, AND/OR APPURTENANCES (I.E., NON-DWELLING FACILITIES/SPACES). DEVELOPMENT ALSO INCLUDES ANY MIXED-FINANCE MODERNIZATION, ALL RELEVANT MODERNIZATION USES (OTHER THAN MANAGEMENT IMPROVEMENTS), FINANCING USES, AND DEVELOPMENT OF NON-DWELLING SPACE WHERE SUCH SPACE IS NEEDED TO ADMINISTER, AND IS OF DIRECT BENEFIT TO A PUBLIC HOUSING PROJECT (I.E. HOUSING DEVELOPED, ACQUIRED, OR ASSISTED BY A PHA UNDER THE 1937 ACT, AND THE IMPROVEMENT OF ANY SUCH HOUSING), INCLUDING THE RESIDENTS. FINANCING DEBT AND FINANCING COSTS (E.G., ORIGINATION FEES, INTEREST) INCURRED BY A PHA FOR DEVELOPMENT OR MODERNIZATION OF PUBLIC HOUSING PROJECTS, INCLUDING MIXED-FINANCE DEVELOPMENT, THE CAPITAL FUND FINANCING PROGRAM (CFFP), AND ANY OTHER USE AUTHORIZED UNDER SECTION 30 OF THE 1937 ACT. MODERNIZATION INCLUDES ALL ELIGIBLE ACTIVITIES EXCEPT FOR DEVELOPMENT AND FINANCING. PHYSICAL WORK IS A MAJOR ACTIVITY AND IS WORK THAT IS DONE ON THE PHYSICAL STRUCTURES, SITE, AND GROUNDS OF A PUBLIC HOUSING PROPERTY OR STRUCTURE. MAJOR PHYSICAL ACTIVITIES INCLUDE DEMOLITION, RECONFIGURATION, EMERGENCY ACTIVITIES, ENERGY EFFICIENCY, NON-ROUTINE MAINTENANCE, PLANNED CODE COMPLIANCE, AND VACANCY REDUCTION. THE MEASURABLE OUTCOME OF THIS GRANT IS THAT HUD WILL BE ABLE TO TRACK THE AMOUNT OF DOLLARS SPENT ON IMPROVEMENTS TO THE STRUCTURES, UNITS, COMMON AREAS, UTILITIES, AND OTHER ELIGIBLE ACTIVITIES. ; EXPECTED OUTCOMES: THE EXPECTED OUTCOMES FOR PUBLIC HOUSING CAPITAL FUNDS OF APPROXIMATELY $3.2 BILLION WILL BE PUT INTO THE DEVELOPMENT, MODERNIZATION, AND FINANCING OF NEARLY 1 MILLION PUBLIC HOUSING UNITS ACROSS ALL 50 STATES AND TERRITORIES. THE PUBLIC HOUSING UNITS ARE UPDATED TO BE DECENT, SAFE, SANITARY AND TO COMPLY WITH FEDERAL HOUSING STANDARDS. PHAS CAN ALSO USE A PORTION OF THE CAPITAL FUNDING FOR MANAGEMENT IMPROVEMENTS OR OPERATING ACTIVITIES INCLUDING SAFETY AND SECURITY COSTS.; INTENDED BENEFICIARIES: THE INTENDED BENEFICIARIES FOR PUBLIC HOUSING CAPITAL FUNDS ARE THE LOW-INCOME PUBLIC HOUSING RESIDENTS.; SUBRECIPIENT ACTIVITIES: THE RECIPIENT DOES NOT INTEND TO SUBAWARD FUNDS.

PURPOSE: THE PUBLIC HOUSING CAPITAL FUND PROGRAM (CFP) WAS CREATED BY AN AMENDMENT TO THE 1937 ACT BY THE QUALITY HOUSING AND WORK RESPONSIBILITY ACT (QHWRA) IN 1998 (ADDING SECTION 9(D) TO THE 1937 ACT MERGING PREVIOUS MODERNIZATION AND DEVELOPMENT PROGRAMS). THE CFP PROVIDES FINANCIAL ASSISTANCE IN THE FORM OF GRANTS TO APPROXIMATELY 2,770 PUBLIC HOUSING AGENCIES (PHAS), SERVING NEARLY ONE MILLION UNITS, IN ALL 50 STATES AND TERRITORIES, TO CARRY OUT CAPITAL AND MANAGEMENT ACTIVITIES INCLUDING THOSE LISTED IN SECTION 9(D)(1) OF THE UNITED STATES HOUSING ACT OF 1937 (1937 ACT). THE MAIN PURPOSE OF THE CFP FORMULA GRANT IS TO FUND PUBLIC HOUSING MODERNIZATION, DEVELOPMENT, MANAGEMENT IMPROVEMENTS, AND THE OTHER ELIGIBLE ACTIVITIES DESCRIBED IN 24 CFR PART 905. ADDITIONAL INFORMATION ON THE PUBLIC HOUSING CAPITAL FUND IS LOCATED ON THE OFFICE OF CAPITAL IMPROVEMENTS WEBSITE: OFFICE OF CAPITAL IMPROVEMENTS | HUD.GOV / U.S. DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT (HUD) ADDITIONAL INFORMATION ON PUBLIC HOUSING FUNDING CAN BE FOUND BY ACCESSING THE WEBSITE BELOW AND REVIEWING THE PUBLIC HOUSING DASHBOARD LINKED UNDER THE “DATA DASHBOARD AND ANALYTICS”. PUBLIC HOUSING | HUD.GOV / U.S. DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT (HUD); ACTIVITIES TO BE PERFORMED: THE PHAS RECEIVE FEDERAL FUNDS FROM THE U.S. DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT (HUD) TO ADMINISTER THE PUBLIC HOUSING FUND. PUBLIC HOUSING CAPITAL FUNDS MAY ONLY BE USED FOR ACTIVITIES THAT ARE DESCRIBED AS ELIGIBLE ACTIVITIES IN 24 CFR 905.200 AND ARE EITHER SPECIFIED IN AN APPROVED 5-YEAR ACTION PLAN OR APPROVED BY HUD FOR EMERGENCY WORK OR WORK NEEDED BECAUSE OF A NON-PRESIDENTIALLY DECLARED NATURAL DISASTER. PUBLIC HOUSING DEVELOPMENT, MODERNIZATION, AND FINANCING ARE THE MAJOR ACTIVITIES TO BE PERFORMED. DEVELOPMENT IS ACTIVITIES AND RELATED COSTS THAT ADD TO (OR SIGNIFICANTLY RECONFIGURE) PUBLIC HOUSING UNITS IN A PHA’S INVENTORY, INCLUDING CONSTRUCTION AND ACQUISITION OF ADDITIONAL PUBLIC HOUSING UNITS, WITH OR WITHOUT REHABILITATION, AND ANY-AND-ALL UNDERTAKINGS NECESSARY FOR PLANNING, DESIGN, FINANCING, LAND ACQUISITION, DEMOLITION, CONSTRUCTION, OR EQUIPMENT OF PUBLIC HOUSING UNITS, AND RELATED BUILDINGS, FACILITIES, AND/OR APPURTENANCES (I.E., NON-DWELLING FACILITIES/SPACES). DEVELOPMENT ALSO INCLUDES ANY MIXED-FINANCE MODERNIZATION, ALL RELEVANT MODERNIZATION USES (OTHER THAN MANAGEMENT IMPROVEMENTS), FINANCING USES, AND DEVELOPMENT OF NON-DWELLING SPACE WHERE SUCH SPACE IS NEEDED TO ADMINISTER, AND IS OF DIRECT BENEFIT TO A PUBLIC HOUSING PROJECT (I.E. HOUSING DEVELOPED, ACQUIRED, OR ASSISTED BY A PHA UNDER THE 1937 ACT, AND THE IMPROVEMENT OF ANY SUCH HOUSING), INCLUDING THE RESIDENTS. FINANCING DEBT AND FINANCING COSTS (E.G., ORIGINATION FEES, INTEREST) INCURRED BY A PHA FOR DEVELOPMENT OR MODERNIZATION OF PUBLIC HOUSING PROJECTS, INCLUDING MIXED-FINANCE DEVELOPMENT, THE CAPITAL FUND FINANCING PROGRAM (CFFP), AND ANY OTHER USE AUTHORIZED UNDER SECTION 30 OF THE 1937 ACT. MODERNIZATION INCLUDES ALL ELIGIBLE ACTIVITIES EXCEPT FOR DEVELOPMENT AND FINANCING. PHYSICAL WORK IS A MAJOR ACTIVITY AND IS WORK THAT IS DONE ON THE PHYSICAL STRUCTURES, SITE, AND GROUNDS OF A PUBLIC HOUSING PROPERTY OR STRUCTURE. MAJOR PHYSICAL ACTIVITIES INCLUDE DEMOLITION, RECONFIGURATION, EMERGENCY ACTIVITIES, ENERGY EFFICIENCY, NON-ROUTINE MAINTENANCE, PLANNED CODE COMPLIANCE, AND VACANCY REDUCTION. THE MEASURABLE OUTCOME OF THIS GRANT IS THAT HUD WILL BE ABLE TO TRACK THE AMOUNT OF DOLLARS SPENT ON IMPROVEMENTS TO THE STRUCTURES, UNITS, COMMON AREAS, UTILITIES, AND OTHER ELIGIBLE ACTIVITIES. ; EXPECTED OUTCOMES: THE EXPECTED OUTCOMES FOR PUBLIC HOUSING CAPITAL FUNDS OF APPROXIMATELY $3.2 BILLION WILL BE PUT INTO THE DEVELOPMENT, MODERNIZATION, AND FINANCING OF NEARLY 1 MILLION PUBLIC HOUSING UNITS ACROSS ALL 50 STATES AND TERRITORIES. THE PUBLIC HOUSING UNITS ARE UPDATED TO BE DECENT, SAFE, SANITARY AND TO COMPLY WITH FEDERAL HOUSING STANDARDS. PHAS CAN ALSO USE A PORTION OF THE CAPITAL FUNDING FOR MANAGEMENT IMPROVEMENTS OR OPERATING ACTIVITIES INCLUDING SAFETY AND SECURITY COSTS.; INTENDED BENEFICIARIES: THE INTENDED BENEFICIARIES FOR PUBLIC HOUSING CAPITAL FUNDS ARE THE LOW-INCOME PUBLIC HOUSING RESIDENTS.; SUBRECIPIENT ACTIVITIES: THE RECIPIENT DOES NOT INTEND TO SUBAWARD FUNDS.

PERFORM FUNDING SYS

PUBLIC HOUSING CAPITAL FUND

PUBLIC HOUSING CAPITAL FUND

PUBLIC AND INDIAN HOUSING

PURPOSE: THE PUBLIC HOUSING OPERATING FUND (PH OPFUND) PROVIDES OPERATING SUBSIDIES TO HOUSING AUTHORITIES (HAS) TO ASSIST IN FUNDING THE OPERATING AND MAINTENANCE EXPENSES OF THEIR DWELLINGS, IN ACCORDANCE WITH SECTION 9 OF THE U.S. HOUSING ACT OF 1937, AS AMENDED. THE SUBSIDIES ARE REQUIRED TO HELP MAINTAIN SERVICES AND PROVIDE MINIMUM OPERATING RESERVES. THE PH OPFUND IS A $5 BILLION DOLLAR PROGRAM PROVIDING FUNDING TO APPROXIMATELY 6,000 HAS SERVING 1,590,321 PUBLIC HOUSING RESIDENTS IN 902,436 HOUSEHOLDS (44% ARE ELDERLY AND 35% OF RESIDENTS HAVE CHILDREN). INFORMATION ON THE CURRENT OPERATING FUND GRANT PROCESSING CAN BE FOUND AT HTTPS://WWW.HUD.GOV/PROGRAM_OFFICES/PUBLIC_INDIAN_HOUSING/PROGRAMS/PH/AM/FUNDING.; ACTIVITIES TO BE PERFORMED: OPERATING FUNDS ARE USED TO FUND DAY-TO-DAY OPERATIONAL EXPENSES ASSOCIATED WITH PUBLIC HOUSING AS WELL AS THE ADMINISTRATIVE AND PROGRAM IMPLEMENTATION EXPENSES THAT PUBLIC HOUSING AGENCIES (PHAS) ARE REQUIRED TO UNDERTAKE UNDER THE 1937 HOUSING ACT AND PROGRAM REGULATIONS. SUCH ACTIVITIES INCLUDE MANAGEMENT AND OPERATIONS, ROUTINE AND PREVENTATIVE MAINTENANCE, ANTI-CRIME, ANTI-DRUG AND SECURITY ACTIVITIES, OPERATING COSTS FOR PRIVATELY OWNED PUBLIC HOUSING UNITS WITHIN MIXED-FINANCE PROJECTS, ENERGY COSTS, RESIDENT SUPPORTIVE SERVICES, INSURANCE, DEBT SERVICE AND COSTS ASSOCIATED WITH ADMINISTRATION AND PROGRAM IMPLEMENTATION. TURNKEY III PROJECTS ARE FUNDED FOR UNITS UNDER THE FINAL LEASE PURCHASE AGREEMENT FOR CLOSING OUT THE PROGRAM. TO SUPPORT THESE ACTIVITIES, THERE IS CONTINUED MODERNIZATION OF THE INFORMATION TECHNOLOGY PLATFORMS. PHAS HAVE ACCESS TO WEB-BASED PLATFORMS THAT UTILIZE REAL-TIME DATA TO PROVIDE INSIGHT INTO THEIR PORTFOLIOS. PHAS CAN OBTAIN METRICS ON THEIR FUNDING LEVELS, OCCUPANCY RATES, AND THE NUMBER OF FAMILIES SERVED THROUGH RENTAL ASSISTANCE.; EXPECTED OUTCOMES: AS A RESULT OF THE ACTIVITIES PERFORMED, THIS PROGRAM IS EXPECTED TO ASSIST IN FUNDING THE OPERATING AND MAINTENANCE EXPENSES. THIS MAY INCLUDE INCREASED OCCUPANCY IN PUBLIC HOUSING, DECREASED ENERGY COSTS THROUGH REGULAR MAINTENANCE AND ENERGY PERFORMANCE CONTRACTING AND LEVERAGE FEDERAL RESOURCES. IN ADDITION TO ADDRESSING THE DEPARTMENT’S STRATEGIC GOALS OF: • ADDRESSING THE NEED FOR QUALITY AFFORDABLE RENTAL HOMES BY MAINTAINING OR IMPROVING UPON THE 96% OCCUPANCY RATE OF HABITABLE UNITS; • PROMOTING HOUSING AS A PLATFORM TO IMPROVE QUALITY OF LIFE THROUGH SUPPORTIVE SERVICES, CRIME PREVENTION EFFORTS AND RESIDENT ENGAGEMENT ACTIVITIES; AND • HELPING TO BUILD INCLUSIVE COMMUNITIES FREE FROM DISCRIMINATION BY FACILITATING THE IMPLEMENTATION OF AFFIRMATIVELY FURTHERING FAIR HOUSING MEASURES.; INTENDED BENEFICIARIES: THE OPERATING FUND PROVIDES FOR THE OPERATION AND MAINTENANCE OF LOW-INCOME HOUSING PROJECTS TO PHAS/PROJECTS. IT WAS CREATED TO ASSIST HOUSING AUTHORITIES IN PROVIDING DECENT AND SAFE RENTAL HOUSING FOR ELIGIBLE LOW-INCOME FAMILIES OR INDIVIDUALS, THE ELDERLY, AND PERSONS WITH DISABILITIES. A HA DETERMINES ELIGIBILITY BASED ON 1) ANNUAL GROSS INCOME; 2) A PERSON ON WHO IS ELDERLY, A PERSON WITH A DISABILITY, OR AS A FAMILY; AND 3) U.S. CITIZENSHIP OR ELIGIBLE IMMIGRATION STATUS.; SUBRECIPIENT ACTIVITIES: THE SUBRECIPIENT ACTIVITIES ARE UNKNOWN AT THE TIME OF AWARD.

PUBLIC AND INDIAN HOUSING

PUBLIC AND INDIAN HOUSING

PUBLIC AND INDIAN HOUSING

PUBLIC HOUSING CAPITAL FUND

PUBLIC AND INDIAN HOUSING

PERFORM FUNDING SYS

PUBLIC AND INDIAN HOUSING

PERFORM FUNDING SYS

PUBLIC AND INDIAN HOUSING

PUBLIC AND INDIAN HOUSING

THIS PROPOSED PROJECT SEEKS TO BUILD UPON EXISTING RESTORATION EFFORTS BY SUPPLEMENTING AND EXPANDING UPON EXISTING HABITAT RESTORATION PROJECTS IN THE FISH CREEK WATERSHED. IN ADDITION WE WILL TELL THE STORY OF THE RICH HISTORY AND EXTENSIVE MODERN RESTORATION EFFORTS IN FISH CREEK BY DEVELOPING DESIGN PLANS FOR A RESTORATION TRAIL WHICH WILL INCLUDE A SERIES OF INTERPRETIVE SIGNS AND KIOSKS AND IMPROVEMENTS FOR RECREATIONAL ACCESS TO THE CREEK FROM HEADWATERS TO COASTAL AREAS OF THE WATERSHED. WE WILL LEVERAGE THE ONGOING WORK OF THE LAKE SUPERIOR COLLABORATIVE AND AN EXISTING EPA NINE KEY ELEMENT WATERSHED PLANNING PROJECT FOCUSED ON PORTIONS OF THE FISH CREEK WATERSHED TO EXPAND ON EXISTING HABITAT RESTORATION PROJECTS AND THE EDUCATION AND OUTREACH OUTPUTS FROM THE PLANNING EFFORT. WE WILL RESTORE UP TO 10 ACRES OF WETLAND RIPARIAN AND UPLAND HABITAT THROUGH A COMBINATION OF SUPPLEMENTING AND EXPANDING AN EXISTING NONNATIVE CATTAIL REMOVAL AND NATIVE PLANT SEEDING PROJECT IN THE FISH CREEK SLOUGHS TREE OR SHRUB PLANTINGS TO RESTORE TERRESTRIAL AREAS RECENTLY TREATED FOR INVASIVE SPECIES REMOVAL IN PRENTICE PARK IN THE CITY OF ASHLAND AND TREE AND SHRUB PLANTINGS TO ENHANCE EXISTING BLUFF STABILIZATION AND FISH HABITAT ENHANCEMENT PROJECTS ALONG THE UPPER MAIN STEM OF NORTH FISH CREEK. TO COMPLETE THE DESIGN PLANNING FOR THE FISH CREEK RESTORATION TRAIL WE WILL CREATE A RESTORATION TRAIL WORKING GROUP WITH A CHARGE TO OUTLINE KEY COMPONENTS LOCATIONS AND PARTNERS TO INCLUDE IN A RESTORATION TRAIL CONCEPT OUTLINE. A PARTICULAR AREA OF FOCUS WILL BE UPDATING EXISTING INTERPRETIVE SIGNAGE ALONG THE CITY OF ASHLAND LAKEFRONT TRAIL AND WITHIN PRENTICE PARK. A LANDSCAPE DESIGN FIRM AND A CONSULTANT TO ASSIST WITH CONTENT AND DESIGN FOR INTERPRETIVE SIGNAGE WILL BE HIRED TO WORK WITH THE RESTORATION TRAIL WORKING GROUP TO TAKE THE CONCEPT OUTLINE AND GENERATE AT LEAST THREE CONCEPTUAL DESIGNS FOR LOCATIONS AND COMPONENTS IN THE RESTORATION TRAIL. THE RESTORATION TRAIL WORKING GROUP AND CONSULTANTS WILL ENGAGE IN COMMUNITY OUTREACH AND ENGAGEMENT EVENTS TO GATHER COMMUNITY INPUT ON THE DESIGN PLANS AND IDENTIFY A PREFERRED OPTION. THE OUTCOME FROM THE PROJECT WILL BE APPROXIMATELY 30 PERCENT DESIGN PLANS FOR THE RESTORATION TRAIL WITH ITEMIZED COST ESTIMATES AND A TASK LIST FOR IMPLEMENTING THE FULL DESIGN. THE DESIGN PLANS AND COST ESTIMATE WILL ALLOW PARTNERS TO APPLY FOR FUNDING TO IMPLEMENT THE FULL DESIGN OF THE RESTORATION TRAIL. THIS PROJECT DIRECTLY SUPPORTS MULTIPLE CONSERVATION PRIORITIES AND OBJECTIVES IDENTIFIED BY THE HEADWATERS TO COAST INITIATIVE INCLUDING CONNECTED AND FUNCTIONAL HABITATS HEALTHY HABITATS COMMUNITY ENGAGEMENT AND PREPARE FOR AND ADDRESS CURRENT AND EMERGING THREATS.

THIS ACTION APPROVES AN AWARD IN THE AMOUNT OF $300,000 TO THE COUNTY OF BURKE, NORTH CAROLINA TO CONDUCT COMMUNITY-WIDE ASSESSMENTS AT BROWNFIELDS SITES POTENTIALLY CONTAMINATED WITH HAZARDOUS SUBSTANCES AND/OR PETROLEUM PRODUCTS. THE RECIPIENT MAY ALSO DEVELOP A SITE INVENTORY, PRIORITIZE SITES AND CONDUCT PHASE I AND/OR PHASE II ASSESSMENTS. FUNDS MAY ALSO BE USED FOR PUBLIC OUTREACH, COMMUNITY INVOLVEMENT, AND CLEANUP/REUSE PLANNING ACTIVITIES.

PUBLIC AND INDIAN HOUSING

PERFORM FUNDING SYS

PUBLIC AND INDIAN HOUSING

ROLE OF SPARC IN WOUND HEALING AFTER SPINAL CORD INJURY - PROJECT SUMMARY: SPINAL CORD INJURY (SCI) TRIGGERS A CELLULAR AND EXTRACELLULAR MATRIX (ECM) REMODELING RESPONSE TO CLOSE THE WOUND. THIS RESPONSE IS DRIVEN LARGELY BY ASTROCYTES FORMING A PROTECTIVE BORDER AROUND INFILTRATING CELLS. THIS PROCESS IS INCOMPLETE IN MOST MAMMALS, FAILING TO RESTORE GROWTH PROMOTING SUBSTRATES, AND LEADING TO EXCESSIVE FIBROSIS. FIBROSIS AFTER SCI IS CHARACTERIZED BY THE SECRETION AND DEPOSITION OF ECM COMPONENTS (EG. COLLAGEN) AND THE ESTABLISHMENT OF A CHRONIC SCAR, IN WHICH CONNECTIVE TISSUE REPLACES NORMAL PARENCHYMA. CONSERVED ROLES FOR NON-NEURAL WOUND HEALING MECHANISMS HAVE BEEN IDENTIFIED IN THE ASTROCYTE RESPONSE TO SCI, BUT THE MOLECULAR CUES THAT DRIVE FIBROSIS AFTER SCI ARE NOT FULLY CHARACTERIZED AND REPRESENT A CRITICAL GAP IMPEDING THE DEVELOPMENT OF STRATEGIES TO IMPROVE SPINAL CORD WOUND HEALING. THE LONG-TERM GOAL IS TO DEVELOP THERAPIES THAT PROMOTE WOUND CLOSURE AND LIMIT FIBROTIC PROCESSES THAT LEAD TO PERSISTENT SCARRING. SPARC (SECRETED PROTEIN ACIDIC AND CYSTEINE RICH) IS A CENTRAL MODULATOR OF BOTH WOUND HEALING AND FIBROSIS. THERE IS A ROBUST AND PERSISTENT INCREASE IN SPARC PROTEIN EXPRESSION IN BORDER-ASSOCIATED ASTROCYTES AFTER SCI AND CONSTITUTIVE KNOCKOUT OF SPARC IMPAIRS WOUND HEALING AND IMPACTS BOTH CELLULAR AND EXTRACELLULAR REMODELING. THE OVERALL OBJECTIVE OF THIS PROPOSAL IS TO DETERMINE THE ROLE OF ASTROCYTIC SPARC SIGNALING IN THE STRUCTURAL AND FUNCTIONAL RESPONSES TO SCI. THE CENTRAL HYPOTHESIS IS THAT ASTROCYTIC SPARC HAS AN ACUTE, EARLY, PRO-WOUND HEALING ROLE AND A SUB-ACUTE, LATE, PRO-FIBROTIC ROLE FOLLOWING SCI. THE FOLLOWING TWO SPECIFIC AIMS ARE PROPOSED: 1) CHARACTERIZE THE TEMPORAL ROLES OF ASTROCYTIC SPARC IN WOUND REMODELING FOLLOWING SCI; 2) DETERMINE THE EFFECT OF ACUTE AND SUB-ACUTE SPARC DELETION IN ASTROCYTES ON FUNCTIONAL RECOVERY AFTER SCI. THE APPROACH IN AIM 1 WILL BE TO TARGET ACUTE AND SUB-ACUTE SPARC EXPRESSION IN ASTROCYTES USING TEMPORAL CONTROL IN A CONDITIONAL KNOCKOUT MOUSE MODEL TO EVALUATE CELLULAR RESPONSES AND ECM COMPOSITION FOLLOWING CONTUSIVE SCI. IN AIM 2, THE APPROACH WILL BE TO ASSESS SENSORY AND MOTOR RECOVERY IN CONTROL AND ASTROCYTE-SPECIFIC CONDITIONAL KNOCKOUT MICE WITH ACUTE OR SUB-ACUTE SPARC DELETION FOLLOWING CONTUSIVE SCI. THE PROPOSED RESEARCH IS INNOVATIVE IN THAT IT CENTERS ON DEFINING THE TEMPORAL AND CELL-TYPE SPECIFICITY FOR A KEY DRIVER OF EPITHELIAL WOUND HEALING AND FIBROSIS IN THE CONTEXT OF SCI, CONNECTING THE FIELDS OF NON-NEURAL AND NEURAL WOUND HEALING. THE PROPOSED STUDIES ARE SIGNIFICANT AS AN UNDERSTANDING OF CONSERVED ROLES FOR NON- NEURAL WOUND HEALING PATHWAYS AFTER SCI, MAY LEAD TO THE DEVELOPMENT OF NEW THERAPEUTIC APPROACHES TO ALTER SCI PROGRESSION, ENHANCE REMODELING, AND LIMIT PATHOLOGICAL SCARRING. THE EXPECTATION IS THAT COMPLETION OF THE PROPOSED STUDIES WILL DETERMINE THE ROLE OF ASTROCYTE-DERIVED SPARC IN WOUND HEALING AND FIBROSIS AFTER SCI. THE IDENTIFICATION OF NEW REGULATORS OF WOUND HEALING AND FIBROSIS WILL ALLOW FOR THE SUBSEQUENT DEVELOPMENT OF THERAPIES TO INCREASE REPAIR BY REDUCING THE GROWTH-RESTRICTIVE ENVIRONMENT AFTER SCI.

AN ADVANCED, HIGH-THROUGHPUT IMAGING SYSTEM FOR THE BNI STRUCTURAL AND FUNCTIONAL IMAGING CORE - THIS APPLICATION IS A REQUEST FOR FUNDS TO ACQUIRE A ZEISS AXIOSCAN 7 SLIDE SCANNER FOR THE STRUCTURAL AND FUNCTIONAL IMAGING CORE AT THE BURKE NEUROLOGICAL INSTITUTE. THE INSTITUTE, DEDICATED TO FINDING CURES FOR CHRONIC NEUROLOGICAL DISABILITIES, REQUIRES AN AUTOMATED IMAGE ACQUISITION SYSTEM TO ADVANCE THEIR NIH-FUNDED RESEARCH. THE MANDATE OF THE IMAGING CORE FACILITY IS TO PROVIDE ACCESS TO A RANGE OF LIGHT MICROSCOPY SYSTEMS AND PROVIDE TECHNICAL ASSISTANCE WITH IMAGE ACQUISITION, PROCESSING, AND ANALYSIS TO ALL RESEARCH GROUPS WITHIN BNI. WHILE THE INSTITUTE HAS INVESTED IN HIGH-RESOLUTION CONFOCAL AND MULTIPHOTON MICROSCOPY SYSTEMS, IT LACKS MODERN EPIFLUORESCENCE AND BRIGHTFIELD IMAGING SYSTEMS. EXISTING MICROSCOPY SOLUTIONS ARE AGING AND LACK CRITICAL FEATURES FOUND IN THE PROPOSED AXIOSCAN 7 SYSTEM. THIS SCARCITY OF RESOURCES POSES A SIGNIFICANT BOTTLENECK FOR THE DIVERSE NIH-FUNDED RESEARCH PROGRAMS AT THE INSTITUTE. THE AXIOSCAN 7 SLIDE SCANNER, WITH ITS AUTOMATED FULL SLIDE WORKFLOW, UNLIMITED Z-STACK ACQUISITION PARAMETERS, AND ADVANCED DECONVOLUTION ALGORITHMS, OFFERS AN OPTIMAL COMBINATION OF FEATURES FOR LARGE AREA TISSUE SCANNING AND STITCHING. THE PROPOSED SYSTEM WILL GREATLY EXPEDITE RESEARCH PROGRESS BY REDUCING ACTIVE USER TIME DURING IMAGE ACQUISITION. FURTHERMORE, THE SURROUNDING AREA LACKS OTHER AUTOMATED, HIGH-THROUGHPUT MICROSCOPY RESOURCES ACCESSIBLE TO BNI INVESTIGATORS, MAKING THE AXIOSCAN 7 AN INDISPENSABLE ASSET. ITS IMPLEMENTATION WILL NOT ONLY BENEFIT CURRENTLY NIH-FUNDED RESEARCH PROGRAMS BUT ALSO CATALYZE THE PROGRESS OF STUDIES THAT ARE YET TO REACH THE MATURITY REQUIRED FOR NIH FUNDING. THE PROPOSED RESEARCH PROJECTS UNDERSCORE THE SHARED NEED FOR AN ADVANCED SLIDE SCANNING SYSTEM. IN CONCLUSION, THE EXTENSIVE CAPABILITIES AND EFFICIENT WORKFLOW OF THE AXIOSCAN 7 WILL EXERT A SUSTAINED, POWERFUL INFLUENCE ON THE CONDUCT OF NIH-FUNDED RESEARCH PROGRAMS AT THE BURKE NEUROLOGICAL INSTITUTE AND SUPPORT BREAKTHROUGHS IN THE UNDERSTANDING AND TREATMENT OF CHRONIC NEUROLOGICAL DISABILITIES.

JOBS-PLUS PILOT INITIATIVE

PURPOSE: CONGRESS ANNUALLY APPROPRIATES FUNDS FOR THE CAPITAL FUND, INCLUDING THE EMERGENCY AND NON-PRESIDENTIALLY DECLARED NATURAL DISASTER SET-ASIDE. THE ANNUAL APPROPRIATIONS ACTS ALSO PERMITS HUD TO USE A PORTION OF THE EMERGENCY AND NON-PRESIDENTIALLY DECLARED NATURAL DISASTER SET-ASIDE FOR SAFETY AND SECURITY FUNDING. HUD AWARDS EMERGENCY AND NON-PRESIDENTIALLY DECLARED NATURAL DISASTER GRANTS TO COVER CAPITAL NEEDS ARISING FROM EMERGENCIES AND NON-PRESIDENTIALLY DECLARED NATURAL DISASTERS OCCURRING IN THE SAME FEDERAL FISCAL YEAR (FFY). FOR EXAMPLE, PUBLIC HOUSING AGENCIES (PHAS) WITH CAPITAL NEEDS ARISING FROM A NON-PRESIDENTIALLY DECLARED NATURAL DISASTER IN FFY 2023 MAY ONLY RECEIVE FUNDS FROM THE FFY 2023 EMERGENCY AND NON-PRESIDENTIALLY DECLARED NATURAL DISASTER SET-ASIDE. APPROXIMATELY 2,770 PHAS SERVING NEARLY ONE MILLION UNITS, IN ALL 50 STATES AND TERRITORIES ARE ELIGIBLE TO APPLY. GRANTS ARE AWARDED ON A FIRST-COME, FIRST-SERVED BASIS UNTIL THE EMERGENCY AND NON-PRESIDENTIALLY DECLARED NATURAL DISASTER SET-ASIDE FUNDS ARE EXHAUSTED. INSURANCE COVERAGE, WHICH IS REQUIRED BY THE ANNUAL CONTRIBUTIONS CONTRACT (ACC), SHOULD BE A PHA’S PRIMARY SOURCE OF FUNDING FOR REPAIR OR REPLACEMENT COSTS ASSOCIATED WITH EMERGENCIES AND NON-PRESIDENTIALLY DECLARED NATURAL DISASTERS. PHAS ARE ELIGIBLE FOR PERMANENT RECONSTRUCTION ASSISTANCE THROUGH THE FEDERAL EMERGENCY MANAGEMENT AGENCY (FEMA) FOR PRESIDENTIALLY-DECLARED NATURAL DISASTERS. FEMA MAY ALSO PROVIDE EMERGENCY ASSISTANCE DURING NON-PRESIDENTIALLY DECLARED NATURAL DISASTERS FOR CERTAIN ACTIVITIES SUCH AS DEBRIS REMOVAL, DEMOLITION OF UNSAFE STRUCTURES, AND ANY ACTIONS NECESSARY TO REDUCE AN IMMEDIATE THREAT TO LIFE, PROPERTY, AND PUBLIC HEALTH AND SAFETY. ADDITIONAL INFORMATION ON THE PUBLIC HOUSING CAPITAL FUND IS LOCATED ON THE OFFICE OF CAPITAL IMPROVEMENTS WEBSITE: HTTPS://WWW.HUD.GOV/PROGRAM_OFFICES/PUBLIC_INDIAN_HOUSING/PROGRAMS/PH/CAPFUND ADDITIONAL INFORMATION ON PUBLIC HOUSING FUNDING CAN BE FOUND BY ACCESSING HTTPS://WWW.HUD.GOV/PROGRAM_OFFICES/PUBLIC_INDIAN_HOUSING/PROGRAMS/PH AND REVIEWING THE PUBLIC HOUSING DASHBOARD LINKED UNDER THE “DATA DASHBOARD AND ANALYTICS”.; ACTIVITIES TO BE PERFORMED: THE DEFINITIONS OF “EMERGENCY” AND “NON-PRESIDENTIALLY DECLARED NATURAL DISASTER” ARE USED IN DETERMINING ELIGIBILITY FOR EMERGENCY AND NON-PRESIDENTIALLY DECLARED NATURAL DISASTER GRANTS: • EMERGENCY: AN UNFORESEEN OR UNPREVENTABLE EVENT OR OCCURRENCE THAT POSES AN IMMEDIATE THREAT TO THE HEALTH AND SAFETY OF THE RESIDENTS (INCLUDING FIRE SAFETY) THAT MUST BE CORRECTED WITHIN ONE YEAR OF FUNDING. EXAMPLES OF EMERGENCIES ARE BOILER FAILURE, WATER INTRUSION CAUSING MOLD GROWTH, SEWER LINE FAILURE, AND SEVERE ELECTRICAL PROBLEMS. A PHA MUST BE ABLE TO DEMONSTRATE THAT IT HAS PROPERLY MAINTAINED STRUCTURES AND EQUIPMENT. • NON-PRESIDENTIALLY DECLARED NATURAL DISASTER: AN EXTRAORDINARY EVENT, SUCH AS AN EARTHQUAKE, FLOOD, OR HURRICANE, AFFECTING A PUBLIC HOUSING PROPERTY OWNED AND MANAGED BY A PHA, BUT EXCLUDING AREAS COVERED BY A PRESIDENTIALLY-DECLARED EMERGENCIES AND MAJOR DISASTERS UNDER THE ROBERT T. STAFFORD DISASTER RELIEF AND EMERGENCY ASSISTANCE ACT, AS THOSE AREAS ARE ELIGIBLE FOR FUNDING FROM THE FEDERAL MANAGEMENT AGENCY (FEMA). CAPITAL FUND DISASTER FUNDING CAN ONLY BE USED FOR ACTIVITIES AND COSTS ASSOCIATED WITH THE REPAIR OR REPLACEMENT OF A PUBLIC HOUSING PROJECT ONLY IF THE PUBLIC HOUSING PROJECT WAS DAMAGED BECAUSE OF AN EMERGENCY OR NON-PRESIDENTIALLY DECLARED NATURAL DISASTER. ACTIVITIES TO BE PERFORMED ARE TO PROVIDE EMERGENCY SAFETY MEASURES FOR PUBLIC HOUSING RESIDENTS THAT CAN INCLUDE RELOCATION AND THE COST TO PROVIDE TEMPORARY HOUSING TO PUBLIC HOUSING RESIDENTS UNTIL PUBLIC HOUSING UNITS CAN BE REPAIRED AND CAN BE COVERED BY THE EMERGENCY AND NON-PRESIDENTIALLY DECLARED NATURAL DISASTER GRANT (SUCH AS HOTEL, MOVING VAN RENTAL, TEMPORARY STORAGE, BOXES.). ACTIVITIES TO BE PERFORMED ARE CONTAINED WITHIN THE GRANTEE’S APPROVED GRANT APPLICATION. EXAMPLES OF TYPICAL EMERGENCY SITUATIONS CAN INCLUDE BUT NOT LIMITED TO ELEVATOR FAILURES IN ELDERLY BUILDINGS, WATER PIPE BURST AND FLOODING SITUATIONS, SEWAGE BACKUP AND INTRUSIONS, SEISMIC ACTIVITY DAMAGE, TREE ROOT INTRUSION, FAILED HVAC SYSTEM, AND BOILER LOSS DURING COLD WEATHER. ; EXPECTED OUTCOMES: THE EXPECTED OUTCOMES FOR PUBLIC HOUSING EMERGENCY/DISASTER CAPITAL FUNDS ARE THE IMMEDIATE SAFETY MEASURES FOR PUBLIC HOUSING RESIDENTS AND THE REMEDIATION OF THE SPECIFIC APPROVED EMERGENCY/DISASTER PROJECT. THE MEASURABLE OUTCOME OF THIS GRANT IS THAT HUD WILL BE ABLE TO TRACK THE AMOUNT OF DOLLARS SPENT ON EMERGENCY AND DISASTER ELIGIBLE ACTIVITIES BASED ON THE APPROVED GRANT APPLICATION. ; INTENDED BENEFICIARIES: THE INTENDED BENEFICIARIES FOR PUBLIC HOUSING EMERGENCY/DISASTER CAPITAL FUNDS ARE LOW-INCOME PUBLIC HOUSING RESIDENTS.; SUBRECIPIENT ACTIVITIES: THE RECIPIENT DOES NOT INTEND TO SUBAWARD FUNDS.

CONVERTING LOCALLY COLLECTED WASTE PLASTICS INTO A CLEAN, DURABLE GROWTH MEDIUM FOR ORCHIDS

PUBLIC HOUSING CAPITAL FUND

READINESS AND EMERGENCY MANAGEMENT FOR SCHOOLS

PUBLIC HOUSING CAPITAL FUND

PURPOSE: THE PUBLIC HOUSING OPERATING FUND (PH OPFUND) PROVIDES OPERATING SUBSIDIES TO HOUSING AUTHORITIES (HAS) TO ASSIST IN FUNDING THE OPERATING AND MAINTENANCE EXPENSES OF THEIR DWELLINGS, IN ACCORDANCE WITH SECTION 9 OF THE U.S. HOUSING ACT OF 1937, AS AMENDED. THE SUBSIDIES ARE REQUIRED TO HELP MAINTAIN SERVICES AND PROVIDE MINIMUM OPERATING RESERVES. THE PH OPFUND IS A $5 BILLION DOLLAR PROGRAM PROVIDING FUNDING TO APPROXIMATELY 6,000 HAS SERVING 1,590,321 PUBLIC HOUSING RESIDENTS IN 902,436 HOUSEHOLDS (44% ARE ELDERLY AND 35% OF RESIDENTS HAVE CHILDREN). INFORMATION ON THE CURRENT OPERATING FUND GRANT PROCESSING CAN BE FOUND AT HTTPS://WWW.HUD.GOV/PROGRAM_OFFICES/PUBLIC_INDIAN_HOUSING/PROGRAMS/PH/AM/FUNDING.; ACTIVITIES TO BE PERFORMED: OPERATING FUNDS ARE USED TO FUND DAY-TO-DAY OPERATIONAL EXPENSES ASSOCIATED WITH PUBLIC HOUSING AS WELL AS THE ADMINISTRATIVE AND PROGRAM IMPLEMENTATION EXPENSES THAT PUBLIC HOUSING AGENCIES (PHAS) ARE REQUIRED TO UNDERTAKE UNDER THE 1937 HOUSING ACT AND PROGRAM REGULATIONS. SUCH ACTIVITIES INCLUDE MANAGEMENT AND OPERATIONS, ROUTINE AND PREVENTATIVE MAINTENANCE, ANTI-CRIME, ANTI-DRUG AND SECURITY ACTIVITIES, OPERATING COSTS FOR PRIVATELY OWNED PUBLIC HOUSING UNITS WITHIN MIXED-FINANCE PROJECTS, ENERGY COSTS, RESIDENT SUPPORTIVE SERVICES, INSURANCE, DEBT SERVICE AND COSTS ASSOCIATED WITH ADMINISTRATION AND PROGRAM IMPLEMENTATION. TURNKEY III PROJECTS ARE FUNDED FOR UNITS UNDER THE FINAL LEASE PURCHASE AGREEMENT FOR CLOSING OUT THE PROGRAM. TO SUPPORT THESE ACTIVITIES, THERE IS CONTINUED MODERNIZATION OF THE INFORMATION TECHNOLOGY PLATFORMS. PHAS HAVE ACCESS TO WEB-BASED PLATFORMS THAT UTILIZE REAL-TIME DATA TO PROVIDE INSIGHT INTO THEIR PORTFOLIOS. PHAS CAN OBTAIN METRICS ON THEIR FUNDING LEVELS, OCCUPANCY RATES, AND THE NUMBER OF FAMILIES SERVED THROUGH RENTAL ASSISTANCE.; EXPECTED OUTCOMES: AS A RESULT OF THE ACTIVITIES PERFORMED, THIS PROGRAM IS EXPECTED TO ASSIST IN FUNDING THE OPERATING AND MAINTENANCE EXPENSES. THIS MAY INCLUDE INCREASED OCCUPANCY IN PUBLIC HOUSING, DECREASED ENERGY COSTS THROUGH REGULAR MAINTENANCE AND ENERGY PERFORMANCE CONTRACTING AND LEVERAGE FEDERAL RESOURCES. IN ADDITION TO ADDRESSING THE DEPARTMENT’S STRATEGIC GOALS OF: • ADDRESSING THE NEED FOR QUALITY AFFORDABLE RENTAL HOMES BY MAINTAINING OR IMPROVING UPON THE 96% OCCUPANCY RATE OF HABITABLE UNITS; • PROMOTING HOUSING AS A PLATFORM TO IMPROVE QUALITY OF LIFE THROUGH SUPPORTIVE SERVICES, CRIME PREVENTION EFFORTS AND RESIDENT ENGAGEMENT ACTIVITIES; AND • HELPING TO BUILD INCLUSIVE COMMUNITIES FREE FROM DISCRIMINATION BY FACILITATING THE IMPLEMENTATION OF AFFIRMATIVELY FURTHERING FAIR HOUSING MEASURES.; INTENDED BENEFICIARIES: THE OPERATING FUND PROVIDES FOR THE OPERATION AND MAINTENANCE OF LOW-INCOME HOUSING PROJECTS TO PHAS/PROJECTS. IT WAS CREATED TO ASSIST HOUSING AUTHORITIES IN PROVIDING DECENT AND SAFE RENTAL HOUSING FOR ELIGIBLE LOW-INCOME FAMILIES OR INDIVIDUALS, THE ELDERLY, AND PERSONS WITH DISABILITIES. A HA DETERMINES ELIGIBILITY BASED ON 1) ANNUAL GROSS INCOME; 2) A PERSON ON WHO IS ELDERLY, A PERSON WITH A DISABILITY, OR AS A FAMILY; AND 3) U.S. CITIZENSHIP OR ELIGIBLE IMMIGRATION STATUS.; SUBRECIPIENT ACTIVITIES: THE SUBRECIPIENT ACTIVITIES ARE UNKNOWN AT THE TIME OF AWARD.

PUBLIC HOUSING CAPITAL FUND

CAPITAL AND MANAGEMENT ACTIVITIES (FORMULA)

"BRAVE" PROGRAM - BURKE REHABILTIAITON ADAPTIVE SPORTS FOR VETERANS PROGRAM DESIGNATED FUNDING: ADAPTIVE SPORTS GENERAL

PUBLIC HOUSING CAPITAL FUND

NORTH FISH CREEK IS A TRIBUTARY TO CHEQUAMEGON BAY OF LAKE SUPERIOR. THE STREAM HAS IMPORTANT COLD-WATER HABITAT POTENTIAL FOR NATIVE BROOK TROUT THAT IS LIMITED BY EXCESS SEDIMENTATION. THE PHASE 3 KEYSTONE BLUFF PROJECT WILL BE THE FIFTH BLUFF STABILIZATION AND FISH HABITAT ENHANCEMENT PROJECT ON PUBLIC PROPERTY ALONG NORTH FISH CREEK SINCE 2018 AND IS PART OF A LONG-TERM EFFORT TO REDUCE CURRENT RATES OF EROSION AND EXCESS SEDIMENTATION BY HALF. COASTAL PROGRAM FUNDS WILL BE USED TO SUPPORT PROJECT CONSTRUCTION AND MANAGEMENT. CONSTRUCTION ACTIVITIES INCLUDE RE-ROUTING THE STREAM CHANNEL THROUGH THE FLOODPLAIN ABOUT 150 FEET FROM THE BLUFF. LARGE WOOD AND ROCK STRUCTURES WILL BE INSTALLED TO PROTECT THE NEW STREAMBANKS AND BED. THE WOOD STRUCTURES WILL BE DESIGNED TO REPLICATE NATURAL WOOD FORMATIONS THAT ALSO PROVIDE CRITICAL AQUATIC HABITAT. WHEN COMPLETED, THE PROJECT WILL RESTORE AND ENHANCE 0.1 MILES OF STREAM CHANNEL, RESTORE AND ENHANCE 0.1 ACRES OF RIVERINE WETLAND, AND PREVENT AN ESTIMATED 2,800 ANNUAL TONS OF SEDIMENT FROM ENTERING THE STREAM. THE PROJECT WILL BENEFIT BROOK TROUT AND OTHER AQUATIC LIFE IN NORTH FISH CREEK AND RECREATIONAL USERS OF THIS CLASS 1 TROUT STREAM ON PUBLIC PROPERTY.

TRANSCRANIAL DIRECT CURRENT STIMULATION AND ROBOTIC TRAINING IN ADULTS WITH CEREBRAL PALSY

IMPACT OF MOTOR CONNECTIVITY ON EFFICACY OF HAND THERAPY IN CONGENITAL HEMIPLEGIA

RURAL HOSPITAL PROVIDER ASSISTANCE PROGRAM - GENERAL OPERATING

THE KNOB SUPINATION TASK: A SENSITIVE TEST OF CORTICOSPINAL FUNCTION IN THE RAT

A SKILLED, AUTOMATED BEHAVIORAL TASK TO ASSESS MOTOR NETWORK FUNCTION DURING IN VIVO IMAGING IN MICE.

REAP RENEWABLE ENERGY SYSTEM (RES) GRANT UNRESTRICTED AMOUNT

ASSISTANCE TO FIREFIGHTERS GRANTS

CAPITAL FUND PROGRAM

CAPITAL FUND PROGRAM

CAPITAL FUND PROGRAM

STRATEGIC PLAN - IMCP

READINESS AND EMERGENCY MANAGEMENT FOR SCHOOLS

WOVEN IN WOOL: THE REBIRTH OF TRADITIONAL COAST SALISH REGALIA [WOVEN IN WOOL: THE REBIRTH OF TRADITIONAL COAST SALISH REGALIA IS A FIRST-OF-ITS-KIND EXHIBIT THAT WILL EMBRACE THE POWER OF WOOL REGALIA IN ALL FORMS. THE BURKE MUSEUM?S SIX-MONTH EXHIBITION WILL FEATURE TRADITIONAL WOOL REGALIA COMMON TO THE 19TH-CENTURY COAST SALISH PEOPLES OF THE PACIFIC NORTHWEST?AN ARTFORM THAT EMERGED FROM OVER A THOUSAND YEARS OF ARTISTIC TRADITION AND BECAME NEARLY EXTINCT UNTIL THE LATE 20TH CENTURY. IN BOTH CONTENT AND CURATORIAL METHODOLOGY, THIS EXHIBITION WILL RESPECT AND EMPOWER THE VOICES, KNOWLEDGE, AND PRACTICES OF SALISH WEAVERS.]

MUSEUMS FOR AMERICA

CAPITAL FUND PROGRAM

AMERICAN RESCUE PLAN

ASSISTANCE TO FIREFIGHTERS GRANT

MEETING STUDENTS WHERE THEY ARE: EXPANDED APPROACHES TO CULTURAL EDUCATION AT THE BURKE MUSEUM [IN RESPONSE TO COVID, IN SUPPORT OF ITS EDUCATIONAL MISSION, AND IN ALIGNMENT WITH THE INSTITUTIONAL GOAL OF DECOLONIZATION, THE BURKE MUSEUM SEEKS NEH FUNDING TO SUPPORT THE RESTORATION OF EDUCATION STAFFING FOR THE 2021-22 ACADEMIC YEAR, IN ORDER TO MEET THE ANTICIPATED INCREASE IN DEMAND FOR VIRTUAL AND ONSITE CULTURAL EDUCATION SERVICES, THROUGH FIELD TRIPS, SELF-GUIDED YOUTH & FAMILY PROGRAMS, THE BURKEMOBILE, AND BURKE BOXES. AS PART OF A RECENT REVAMP OF ALL EDUCATION PROGRAMS, THE BURKE WORKED WITH ITS TRIBAL PARTNERS TO CREATE A SLATE OF OFFERINGS THAT RETURNS VOICE AND AUTHORITY TO NATIVE COMMUNITIES, WHILE ALSO BREATHING NEW LIFE AND EXCITEMENT INTO OBJECT-BASED LEARNING AT THE BURKE. AN NEH GRANT WILL SIGNIFICANTLY STRENGTHEN OUR ABILITY TO GET THESE WAYS OF SEEING AND UNDERSTANDING NATIVE AMERICAN HISTORY, ART, AND CULTURE TO MORE WA STUDENTS, USING A RANGE OF DELIVERY METHODS THAT WORK WITH AND AROUND LIMITATIONS IMPOSED BY COVID-19.] [PURPOSE: DEVELOP K-12 EDUCATIONAL MATERIALS AND PROGRAMMING ON INDIGENOUS KNOWLEDGE AND PERSPECTIVES.  ACTIVITIES TO BE PERFORMED: SCHOLARS, EDUCATORS, AND LOCAL COMMUNITY MEMBERS WILL COLLABORATE ON THE CREATION OF VIRTUAL AND ONSITE EDUCATIONAL PROGRAMMING, A MOBILE EDUCATION SERVICE, AND MAIL-OUT RESOURCE BOXES ON INDIGENOUS HISTORY IN ALIGNMENT WITH K-12 CURRICULUM AND STATE STANDARDS.  EXPECTED OUTCOMES: IMPROVED TEACHING AND LEARNING ABOUT NATIVE AMERICAN HISTORY, ART, AND CULTURE ACROSS THE STATE OF WASHINGTON.  INTENDED BENEFICIARIES: INDIGENOUS COMMUNITY MEMBERS AND VISITORS TO THE MUSEUM.  SUBRECIPIENT ACTIVITIES:??THE RECIPIENT DOES NOT INTEND TO SUBAWARD FUNDS.? ]

PUBLIC HOUSING CAPITAL FUND

REAP RENEWABLE ENERGY SYSTEM (RES) GRANT UNRESTRICTED AMOUNT

ASSISTANCE TO FIREFIGHTERS GRANT

CAPITAL FUND PROGRAM

PUBLIC HOUSING CAPITAL FUND

CAPITAL FUND PROGRAM

CAPITAL FUND PROGRAM

ASSISTANCE TO FIREFIGHTERS GRANT

SUSTAINING ENGAGEMENT WITH THE BURKE MUSEUM'S NATIVE AMERICAN COLLECTIONS IN THE TIME OF COVID-19

ASSISTANCE TO FIREFIGHTERS GRANTS

COMMUNITY INFRASTRUCTURE

RBDG RURAL BUSINESS COOP RURAL ENTERPRISE GRANT

PERFORM FUNDING SYS

LAW ENFORCEMENT EQUIPMENT AND SUPPLIES

PURPOSE: THE FAMILY SELF-SUFFICIENCY PROGRAM (FSS) IS GOVERNED BY SECTION 23 OF THE 1937 HOUSING ACT. THE PURPOSE OF FSS AWARDS IS TO FUND THE SALARIES AND FRINGE FOR FSS COORDINATORS, WHO ARE RESPONSIBLE FOR IMPLEMENTING FSS FOR RESIDENTS OF PUBLIC HOUSING, HOUSING CHOICE VOUCHERS (HCV), AND PROJECT-BASED RENTAL ASSISTANCE (PBRA) PROPERTIES. FSS FUNDS APPROXIMATELY 900 PROGRAMS RUN BY PUBLIC HOUSING AUTHORITIES (PHAS) AND PBRA PROPERTY OWNERS ACROSS THE COUNTRY. RENEWAL APPLICANTS ARE PRIORITIZED BY STATUTE.; ACTIVITIES TO BE PERFORMED: FUNDS UNDER FSS GRANTS ARE USED SOLELY TO FUND SALARY AND FRINGE BENEFITS FOR FSS COORDINATORS. RESPONSIBILITIES OF FSS COORDINATORS UNDER THE AWARD INCLUDE, BUT ARE NOT LIMITED TO, ENROLLING, AND MAINTAINING FAMILIES IN THE PROGRAM, WORKING WITH FAMILIES TO PREPARE INDIVIDUAL TRAINING AND SERVICES PLANS (ITSPS), AND BUILDING PARTNERSHIPS WITH EMPLOYERS AND SERVICE PROVIDERS IN THE COMMUNITY. THE REQUIRED NUMBER OF PARTICIPATING FAMILIES THAT A GRANTEE MUST MAINTAIN IS OUTLINED IN THE FUNDING NOTICE AND IS BASED ON THE NUMBER OF COORDINATORS FOR WHICH A GRANTEE RECEIVES FUNDING. GENERALLY, THE GRANTEE MUST HIRE/RETAIN ONE FULL-TIME EQUIVALENT (FTE) FSS PROGRAM COORDINATOR FOR EACH FUNDED POSITION. FOR THE FIRST FULL-TIME FSS COORDINATOR POSITION FUNDED BY HUD, PROGRAMS MUST SERVE AT LEAST 25 PARTICIPATING FAMILIES IN FSS DURING THE PERIOD OF PERFORMANCE (THE CALENDAR YEAR FOLLOWING THE APPROPRIATION), AND THIS NUMBER INCREASES BY 50 FAMILIES FOR EACH ADDITIONAL FUNDED COORDINATOR AFTER THE FIRST.; EXPECTED OUTCOMES: GRANTEES WILL HIRE/RETAIN THE NUMBER OF COORDINATORS FOR WHICH THEY ARE FUNDED. THESE COORDINATORS WILL SERVE AT LEAST THE MINIMUM NUMBER OF PARTICIPANTS REQUIRED BY THE GRANT. OVER THE 5-7 YEARS OF THEIR CONTRACT OF PARTICIPATION, PARTICIPANTS WILL BECOME EMPLOYED OR IMPROVE THEIR EMPLOYMENT AND WAGES AND MEET OTHER INDIVIDUAL GOALS.; INTENDED BENEFICIARIES: THE INTENDED BENEFICIARIES OF FSS ARE FAMILIES CURRENTLY RESIDING IN PUBLIC HOUSING (SECTION 9), FAMILIES CURRENTLY PARTICIPATING IN THE HOUSING CHOICE VOUCHER (SECTION 8) PROGRAM, AND THOSE LIVING IN PROJECT-BASED RENTAL ASSISTANCE (PBRA) PROPERTIES. ; SUBRECIPIENT ACTIVITIES: THE RECIPIENT DOES NOT INTEND TO SUBAWARD FUNDS.

ELUCIDATING THE MECHANISMS OF NEUROPROTECTION OF HISTONE DEACETYLASE INHIBITION IN ISCHEMIC STROKE

CORTICOSPINAL NEURON PLASTICITY IN RESPONSE TO DEVELOPMENTAL SPINAL CORD AXOTOMY. - PROJECT ABSTRACT SPINAL CORD INJURY (SCI) IS A DEVASTATING CONDITION WITH LIMITED TREATMENT OPTIONS DUE TO THE RESTRICTED REGENERATIVE CAPACITY OF THE MATURE CENTRAL NERVOUS SYSTEM. DUE TO THE HETEROGENEITY OF SCI ALONG THE NEURAXIS, IT IMPAIRS VARIOUS CORTICOSPINAL CIRCUITRY THAT AFFECTS MOTOR CONTROL. THE CORTICOSPINAL TRACT (CST), ESSENTIAL FOR VOLUNTARY MOVEMENT, CONSISTS OF DIVERSE CORTICOSPINAL NEURON (CSN) SUBTYPES, YET THE ESTABLISHMENT OF THESE DISTINCT SPINAL-PROJECTING NEURONS REMAINS UNCLEAR. RECENT FINDINGS FROM THE SAHNI LAB HAVE IDENTIFIED EARLY MOLECULAR DIFFERENCES IN CSN SUBPOPULATIONS PROJECTING TO THE CERVICAL OR THORACOLUMBAR SPINAL CORD FROM CORTICAL REGIONS OUTSIDE THE CLASSICAL MOTOR CORTEX; HOWEVER, THIS MOLECULAR MAP IS INCOMPLETE. ADDITIONALLY, THE LAB HAS DEVELOPED A NOVEL MICROLESION TECHNIQUE TO AXOTOMIZE THE DEVELOPING CST, ENABLING THE STUDY OF CSN PLASTICITY AND CIRCUIT FORMATION DURING DEVELOPMENT. UNDERSTANDING HOW CSN PROJECTION TYPES AND THEIR POSTSYNAPTIC SPINAL CONNECTIVITY ARE ESTABLISHED IS CRUCIAL FOR DEVELOPING STRATEGIES FOR FUNCTIONAL RECOVERY AFTER SCI. THIS PROPOSAL AIMS TO INVESTIGATE THE MOLECULAR MECHANISMS UNDERLYING CSN DEVELOPMENT AND THEIR LONG-RANGE CORTICOSPINAL CIRCUITS. MY CENTRAL HYPOTHESIS IS THAT INTRINSIC REGULATORS GOVERN SEGMENTALLY DISTINCT CSN IDENTITIES AND SPINAL SYNAPTIC CIRCUITRY. SPECIFIC AIM 1 WILL IDENTIFY MOLECULAR REGULATORS OF CSN DIVERSITY AND DETERMINE WHETHER SEGMENTAL SPINAL TARGETING IS GOVERNED BY INTRINSIC MECHANISMS OR TARGET- DERIVED RETROGRADE SIGNALING. SPECIFIC AIM 2 WILL EXPLORE HOW ROUTE OF AXON EXTENSION INFLUENCES SYNAPTIC SPECIFICITY AND THEIR IMPLICATIONS FOR THE EVOLUTION OF CORTICAL-MOTONEURAL CONNECTIONS AND DEXTERITY. TO ACHIEVE THESE AIMS, WE WILL UTILIZE MOUSE MODELS AND EMPLOY TECHNIQUES SUCH AS VIRAL TRACING AND MICROLESION APPROACHES. THE FINDINGS WILL ENHANCE OUR UNDERSTANDING OF NEURODEVELOPMENTAL PROCESSES AND MAY LEAD TO NEW THERAPEUTIC STRATEGIES FOR SCI RECOVERY. WITH THE SUPPORT OF THIS F32 FELLOWSHIP, I WILL STRENGTHEN MY EXPERTISE IN VIRAL TRACING, MOLECULAR PROFILING, AND BIOINFORMATICS, PREPARING ME TO LEAD AN INDEPENDENT RESEARCH PROGRAM FOCUSED ON LEVERAGING DEVELOPMENTAL MECHANISMS TO REPAIR THE INJURED NERVOUS SYSTEM.

PUBLIC AND INDIAN HOUSING

15.945: COOPERATIVE RESEARCH AND TRAINING PROGRAMS OF THE NATIONAL PARK SYSTEM CESU NETWORKPROGRAMMING SUPPORTS A COMPREHENSIVE NETWORK OF COOPERATIVE STUDY UNITS TO FACILITATE COOPERATIVE RESEARCH, TECHNICAL ASSISTANCE, EDUCATION, AND TRAINING TO INFORM SCIENCE-BASED MANAGEMENT OF THE NATIONAL PARK SYSTEM. THE STUDY UNITS FACILITATE MULTI-DISCIPLINARY SCIENCE AND INTEGRATED INFORMATION PRODUCTS ACROSS MULTIPLE GEOGRAPHIC REGIONS OF THE NATIONAL PARK SYSTEM.THROUGH THIS AGREEMENT THE NATIONAL PARK SERVICE WILL OBTAIN FIELD ASSISTANCE FROM THE BURKE CENTER, A NONPROFIT ORGANIZATION THAT FOCUSES ON FRESHWATER ISSUES IN NORTHERN WISCONSIN. SPECIFICALLY, BURKE CENTER STAFF WILL COLLECT WATER SAMPLES FROM LAKE SUPERIOR AND DRAGONFLY SAMPLES (AN EFFICIENT SUBSTITUTE FOR FISH) FROM FOUR NATIONAL PARKS IN THE WESTERN GREAT LAKES REGION. THE WORK WILL HELP PARK MANAGERS UNDERSTAND IF THE WATERS ARE SAFE FOR RECREATION AND FISH ARE SAFE TO EAT. BENEFICIARIES INCLUDE STUDENTS GAINING HANDS-ON EXPERIENCE, THE SCIENTIFIC COMMUNITY EXPANDING KNOWLEDGE ABOUT THE SPECIES, AND THE PUBLIC ACCESSING STUDY FINDINGS.

PUBLIC AND INDIAN HOUSING

RBDG RURAL BUSINESS COOP BUSINESS ENTERPRISE REAP ZONES GRANT

PURPOSE: THE FAMILY SELF-SUFFICIENCY PROGRAM (FSS) IS GOVERNED BY SECTION 23 OF THE 1937 HOUSING ACT. THE PURPOSE OF FSS AWARDS IS TO FUND THE SALARIES AND FRINGE FOR FSS COORDINATORS, WHO ARE RESPONSIBLE FOR IMPLEMENTING FSS FOR RESIDENTS OF PUBLIC HOUSING, HOUSING CHOICE VOUCHERS (HCV), AND PROJECT-BASED RENTAL ASSISTANCE (PBRA) PROPERTIES. FSS FUNDS APPROXIMATELY 900 PROGRAMS RUN BY PUBLIC HOUSING AUTHORITIES (PHAS) AND PBRA PROPERTY OWNERS ACROSS THE COUNTRY. RENEWAL APPLICANTS ARE PRIORITIZED BY STATUTE.; ACTIVITIES TO BE PERFORMED: FUNDS UNDER FSS GRANTS ARE USED SOLELY TO FUND SALARY AND FRINGE BENEFITS FOR FSS COORDINATORS. RESPONSIBILITIES OF FSS COORDINATORS UNDER THE AWARD INCLUDE, BUT ARE NOT LIMITED TO, ENROLLING, AND MAINTAINING FAMILIES IN THE PROGRAM, WORKING WITH FAMILIES TO PREPARE INDIVIDUAL TRAINING AND SERVICES PLANS (ITSPS), AND BUILDING PARTNERSHIPS WITH EMPLOYERS AND SERVICE PROVIDERS IN THE COMMUNITY. THE REQUIRED NUMBER OF PARTICIPATING FAMILIES THAT A GRANTEE MUST MAINTAIN IS OUTLINED IN THE FUNDING NOTICE AND IS BASED ON THE NUMBER OF COORDINATORS FOR WHICH A GRANTEE RECEIVES FUNDING. GENERALLY, THE GRANTEE MUST HIRE/RETAIN ONE FULL-TIME EQUIVALENT (FTE) FSS PROGRAM COORDINATOR FOR EACH FUNDED POSITION. FOR THE FIRST FULL-TIME FSS COORDINATOR POSITION FUNDED BY HUD, PROGRAMS MUST SERVE AT LEAST 25 PARTICIPATING FAMILIES IN FSS DURING THE PERIOD OF PERFORMANCE (THE CALENDAR YEAR FOLLOWING THE APPROPRIATION), AND THIS NUMBER INCREASES BY 50 FAMILIES FOR EACH ADDITIONAL FUNDED COORDINATOR AFTER THE FIRST.; EXPECTED OUTCOMES: GRANTEES WILL HIRE/RETAIN THE NUMBER OF COORDINATORS FOR WHICH THEY ARE FUNDED. THESE COORDINATORS WILL SERVE AT LEAST THE MINIMUM NUMBER OF PARTICIPANTS REQUIRED BY THE GRANT. OVER THE 5-7 YEARS OF THEIR CONTRACT OF PARTICIPATION, PARTICIPANTS WILL BECOME EMPLOYED OR IMPROVE THEIR EMPLOYMENT AND WAGES AND MEET OTHER INDIVIDUAL GOALS.; INTENDED BENEFICIARIES: THE INTENDED BENEFICIARIES OF FSS ARE FAMILIES CURRENTLY RESIDING IN PUBLIC HOUSING (SECTION 9), FAMILIES CURRENTLY PARTICIPATING IN THE HOUSING CHOICE VOUCHER (SECTION 8) PROGRAM, AND THOSE LIVING IN PROJECT-BASED RENTAL ASSISTANCE (PBRA) PROPERTIES. ; SUBRECIPIENT ACTIVITIES: THE RECIPIENT DOES NOT INTEND TO SUBAWARD FUNDS.

FAMILY SELF-SUFFICIENCY PROGRAM

PURPOSE: THE PUBLIC HOUSING OPERATING FUND (PH OPFUND) PROVIDES OPERATING SUBSIDIES TO HOUSING AUTHORITIES (HAS) TO ASSIST IN FUNDING THE OPERATING AND MAINTENANCE EXPENSES OF THEIR DWELLINGS, IN ACCORDANCE WITH SECTION 9 OF THE U.S. HOUSING ACT OF 1937, AS AMENDED. THE SUBSIDIES ARE REQUIRED TO HELP MAINTAIN SERVICES AND PROVIDE MINIMUM OPERATING RESERVES. THE PH OPFUND IS A $5 BILLION DOLLAR PROGRAM PROVIDING FUNDING TO APPROXIMATELY 6,000 HAS SERVING 1,590,321 PUBLIC HOUSING RESIDENTS IN 902,436 HOUSEHOLDS (44% ARE ELDERLY AND 35% OF RESIDENTS HAVE CHILDREN). INFORMATION ON THE CURRENT OPERATING FUND GRANT PROCESSING CAN BE FOUND AT HTTPS://WWW.HUD.GOV/PROGRAM_OFFICES/PUBLIC_INDIAN_HOUSING/PROGRAMS/PH/AM/FUNDING.; ACTIVITIES TO BE PERFORMED: OPERATING FUNDS ARE USED TO FUND DAY-TO-DAY OPERATIONAL EXPENSES ASSOCIATED WITH PUBLIC HOUSING AS WELL AS THE ADMINISTRATIVE AND PROGRAM IMPLEMENTATION EXPENSES THAT PUBLIC HOUSING AGENCIES (PHAS) ARE REQUIRED TO UNDERTAKE UNDER THE 1937 HOUSING ACT AND PROGRAM REGULATIONS. SUCH ACTIVITIES INCLUDE MANAGEMENT AND OPERATIONS, ROUTINE AND PREVENTATIVE MAINTENANCE, ANTI-CRIME, ANTI-DRUG AND SECURITY ACTIVITIES, OPERATING COSTS FOR PRIVATELY OWNED PUBLIC HOUSING UNITS WITHIN MIXED-FINANCE PROJECTS, ENERGY COSTS, RESIDENT SUPPORTIVE SERVICES, INSURANCE, DEBT SERVICE AND COSTS ASSOCIATED WITH ADMINISTRATION AND PROGRAM IMPLEMENTATION. TURNKEY III PROJECTS ARE FUNDED FOR UNITS UNDER THE FINAL LEASE PURCHASE AGREEMENT FOR CLOSING OUT THE PROGRAM. TO SUPPORT THESE ACTIVITIES, THERE IS CONTINUED MODERNIZATION OF THE INFORMATION TECHNOLOGY PLATFORMS. PHAS HAVE ACCESS TO WEB-BASED PLATFORMS THAT UTILIZE REAL-TIME DATA TO PROVIDE INSIGHT INTO THEIR PORTFOLIOS. PHAS CAN OBTAIN METRICS ON THEIR FUNDING LEVELS, OCCUPANCY RATES, AND THE NUMBER OF FAMILIES SERVED THROUGH RENTAL ASSISTANCE.; EXPECTED OUTCOMES: AS A RESULT OF THE ACTIVITIES PERFORMED, THIS PROGRAM IS EXPECTED TO ASSIST IN FUNDING THE OPERATING AND MAINTENANCE EXPENSES. THIS MAY INCLUDE INCREASED OCCUPANCY IN PUBLIC HOUSING, DECREASED ENERGY COSTS THROUGH REGULAR MAINTENANCE AND ENERGY PERFORMANCE CONTRACTING AND LEVERAGE FEDERAL RESOURCES. IN ADDITION TO ADDRESSING THE DEPARTMENT’S STRATEGIC GOALS OF: • ADDRESSING THE NEED FOR QUALITY AFFORDABLE RENTAL HOMES BY MAINTAINING OR IMPROVING UPON THE 96% OCCUPANCY RATE OF HABITABLE UNITS; • PROMOTING HOUSING AS A PLATFORM TO IMPROVE QUALITY OF LIFE THROUGH SUPPORTIVE SERVICES, CRIME PREVENTION EFFORTS AND RESIDENT ENGAGEMENT ACTIVITIES; AND • HELPING TO BUILD INCLUSIVE COMMUNITIES FREE FROM DISCRIMINATION BY FACILITATING THE IMPLEMENTATION OF AFFIRMATIVELY FURTHERING FAIR HOUSING MEASURES.; INTENDED BENEFICIARIES: THE OPERATING FUND PROVIDES FOR THE OPERATION AND MAINTENANCE OF LOW-INCOME HOUSING PROJECTS TO PHAS/PROJECTS. IT WAS CREATED TO ASSIST HOUSING AUTHORITIES IN PROVIDING DECENT AND SAFE RENTAL HOUSING FOR ELIGIBLE LOW-INCOME FAMILIES OR INDIVIDUALS, THE ELDERLY, AND PERSONS WITH DISABILITIES. A HA DETERMINES ELIGIBILITY BASED ON 1) ANNUAL GROSS INCOME; 2) A PERSON ON WHO IS ELDERLY, A PERSON WITH A DISABILITY, OR AS A FAMILY; AND 3) U.S. CITIZENSHIP OR ELIGIBLE IMMIGRATION STATUS.; SUBRECIPIENT ACTIVITIES: THE SUBRECIPIENT ACTIVITIES ARE UNKNOWN AT THE TIME OF AWARD.

RURAL BUSINESS ENTERPRISE GRANTS - EZ/EC

PURPOSE: THE FAMILY SELF-SUFFICIENCY PROGRAM (FSS) IS GOVERNED BY SECTION 306 OF THE ECONOMIC GROWTH, REGULATORY RELIEF, AND CONSUMER PROTECTION ACT (PUBLIC LAW NO: 115-174). THE PURPOSE OF FSS AWARDS IS TO FUND THE SALARIES AND FRINGE FOR FSS COORDINATORS, WHO ARE RESPONSIBLE FOR IMPLEMENTING FSS FOR RESIDENTS OF PUBLIC HOUSING, HOUSING CHOICE VOUCHERS (HCV), AND PROJECT-BASED RENTAL ASSISTANCE (PBRA) PROPERTIES. FSS FUNDS MORE THAN 800 PROGRAMS RUN BY PUBLIC HOUSING AUTHORITIES (PHAS) AND PBRA PROPERTY OWNERS ACROSS THE COUNTRY. RENEWAL APPLICANTS ARE PRIORITIZED BY STATUTE, AND NEW AWARD SELECTION IS MADE BY LOTTERY.; ACTIVITIES TO BE PERFORMED: FUNDS UNDER FSS GRANTS ARE USED SOLELY TO FUND SALARY AND FRINGE BENEFITS FOR FSS COORDINATORS. RESPONSIBILITIES OF FSS COORDINATORS UNDER THE AWARD INCLUDE, BUT ARE NOT LIMITED TO, ENROLLING, AND MAINTAINING THE MINIMUM NUMBER OF FAMILIES IN THE PROGRAM, WORKING WITH FAMILIES TO PREPARE INDIVIDUAL TRAINING AND SERVICES PLANS (ITSPS), AND BUILDING PARTNERSHIPS WITH EMPLOYERS AND SERVICE PROVIDERS IN THE COMMUNITY. THE REQUIRED NUMBER OF PARTICIPATING FAMILIES THAT A PROGRAM MUST MAINTAIN IS OUTLINED IN THE NOTICE OF FUNDING OPPORTUNITY (NOFO) AND IS BASED ON THE NUMBER OF COORDINATORS FOR WHICH A GRANTEE RECEIVES FUNDING. GENERALLY, THE GRANTEE MUST HIRE/RETAIN ONE FULL-TIME EQUIVALENT (FTE) FSS PROGRAM COORDINATOR FOR EACH FUNDED POSITION. FOR THE FIRST FULL-TIME FSS COORDINATOR POSITION FUNDED BY HUD, PROGRAMS MUST SERVE AT LEAST 25 PARTICIPATING FAMILIES IN FSS DURING THE PERIOD OF PERFORMANCE (THE CALENDAR YEAR FOLLOWING THE APPROPRIATION), AND THIS NUMBER INCREASES BY 50 FAMILIES FOR EACH ADDITIONAL FUNDED COORDINATOR AFTER THE FIRST.; EXPECTED OUTCOMES: GRANTEES WILL HIRE/RETAIN THE NUMBER OF COORDINATORS FOR WHICH THEY ARE FUNDED. THESE COORDINATORS WILL SERVE AT LEAST THE MINIMUM NUMBER OF PARTICIPANTS REQUIRED BY THE GRANT. OVER THE 5-7 YEARS OF THEIR CONTRACT OF PARTICIPATION, PARTICIPANTS WILL BECOME EMPLOYED OR IMPROVE THEIR EMPLOYMENT AND WAGES AND MEET OTHER INDIVIDUAL GOALS.; INTENDED BENEFICIARIES: THE INTENDED BENEFICIARIES OF FSS ARE FAMILIES CURRENTLY RESIDING IN PUBLIC HOUSING (SECTION 9), FAMILIES CURRENTLY PARTICIPATING IN THE HOUSING CHOICE VOUCHER (SECTION 8) PROGRAM, AND THOSE LIVING IN PROJECT-BASED RENTAL ASSISTANCE (PBRA) PROPERTIES.; SUBRECIPIENT ACTIVITIES: THE RECIPIENT DOES NOT INTEND TO SUBAWARD FUNDS.

PUBLIC AND INDIAN HOUSING